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Spatial segregation of transport and signalling functions between human endothelial caveolae and lipid raft proteomes

...electrophoresis preceding identification of proteins by MS was used to compare the relative abundance of proteins in DRMs (detergent-resistant membranes) isolated from HUVEC (human umbilical-vein endothelial cells), and caveolae immunopurified from DRM fractions...

Authors: Sprenger RR, Fontijn RD, van Marle J, Pannekoek H, Horrevoets AJ

Human cytomegalovirus UL130 protein promotes endothelial cell infection through a producer cell modification of the virion

...To provide UL130 in trans to viral infections, we constructed human embryonic lung fibroblast (HELF) and human umbilical vein endothelial cell (HUVEC) derivative cell lines that express UL130 via a retroviral vector. When the UL130-negative virus was grown...

Authors: Patrone M, Secchi M, Fiorina L, Ierardi M, Milanesi Ga and Gallina A

LAT1 is a central transporter of essential amino acids in human umbilical vein endothelial cells

...endothelial cells. Here we show that L-type amino acid transporter 1 (LAT1) is a major transporter of essential amino acids in human umbilical vein endothelial cells (HUVECs). Growing HUVECs express a certain level of LAT1. A LAT1-specific inhibitor suppressed...

Authors: Hayashi K, Jutabha P, Kamai T, Endou H, Anzai N

Impact of serum high mobility group box 1 and soluble receptor for advanced glycation end-products on subclinical atherosclerosis in patients with granulomatosis with polyangiitis

...thickness (IMT). In vitro the effect of atorvastatin on the production of HMGB1 by lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVEC) was assessed. Serum HMGB1 and sRAGE levels did not differ between patients and controls...

Authors: Souza AW, de Leeuw K, van Timmeren MM, Limburg PC, Stegeman CA, Bijl M, Westra J, Kallenberg CG

Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis.

...cells (HUVEC).METHODS:Primary RASFC and HUVEC were cultured with the oxidative stress inducer 4-hydroxy-2-nonenal (4-HNE), and extracellular acidification rate, oxygen consumption rate, mitochondrial function and pro-angiogenic/pro-inflammatory...

Authors: Balogh E1, Veale DJ2, McGarry T3, Orr C2, Szekanecz Z1, Ng CT4,5, Fearon U3, Biniecka M6

The superoxide-producing NAD(P)H oxidase Nox4 in the nucleus of human vascular endothelial cells

...sensing and cellular senescence. Although the oxidase is also abundant in vascular endothelial cells, its role remains to be elucidated. Here we show that Nox4 preferentially localizes to the nucleus of human umbilical vein endothelial cells (HUVECs...

Authors: Kuroda J, Nakagawa K, Yamasaki T, Nakamura K, Takeya R, Kuribayashi F, Imajoh-Ohmi S, Igarashi K,...

Exosomal shuttling of miR-126 in endothelial cells modulates adhesive and migratory abilities of chronic myelogenous leukemia cells

...to be over-overexpressed in exosomes compared with producing parental cells. Transfection of LAMA84 with Cy3-labelled miR-126 and co-culture of leukemia cells with endothelial cells (EC) confirmed that miR-126 is shuttled into HUVECs. The treatment of HUVECs with LAMA84...

Authors: Taverna S, Amodeo V, Saieva L, Russo A, Giallombardo M, De Leo G, Alessandro R

Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by 1 integrins

...umbilical vein endothelial cells (HUVEC), cells that lack CD36. Moreover, we identified beta(1) integrins as a critical receptor in TSR-mediated inhibition of migration in HUVEC. Using pharmacological inhibitors of downstream VEGF receptor effectors, we...

Authors: Short SM, Derrien A, Narsimhan RP, Lawler J, Ingber DE and Zetter BR

An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells.

...model including human osteoblasts (OBs), osteoclasts (OCs), and endothelial cells (HUVEC). While OBs are essential for bone deposition and OCs for bone resorption, the vasculature is necessary to provide growth factors, nutrients, and oxygen...

Authors: Pagani S1, Torricelli P1, Veronesi F1, Salamanna F2, Cepollaro S1,3, Fini M1

Specific Inhibition of Nuclear Factor-B-Dependent Inflammatory Responses by Cell Type-Specific Mechanisms upon A2A Adenosine Receptor Gene Transfer

...expression suppressed multiple inflammatory responses in both human umbilical vein endothelial cells (HUVECs) and rat C6 glioma cells in vitro. In particular, the induction of the adhesion molecule E-selectin by either tumor necrosis factor alpha (TNFalpha...

Authors: Sands WA, Martin AF, Strong EW and Palmer TM

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