Search Results

Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells

...activities.METHODS:The effects of plocabulin on microtubule network and dynamics were studied on HUVEC endothelial cells. We have also studied its effects on capillary tube structures formation or destabilization in three-dimensional collagen matrices. In vivo...

Authors: Galmarini CM1, Martin M2, Bouchet BP2, Guillen-Navarro MJ3, Martínez-Diez M3, Martinez-Leal JF3,...

Engineering a 3D microfluidic culture platform for tumor-treating field application.

The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as...

Authors: Pavesi A, Adriani G, Tay A, Warkiani ME, Yeap WH5, Wong SC5, Kamm RD

SST Peer Reviewed

Shear stress-induced endothelial cell polarization in mediated by Rho and Rac but not Cdc42 or PI 3-kinase

Shear stress induces endothelial polarization and migration in the direction of flow accompanied by extensive remodeling of the actin cytoskeleton. The GTPases RhoA, Rac1, and Cdc42 are known to regulate cell shape changes through effects on the...

Authors: Wojciak-Stothard B and Ridley AJ

COSMC is overexpressed in proliferating infantile hemangioma and enhances endothelial cell growth via VEGFR2

...endothelial cells (HUVECs). Conversely, knockdown of COSMC with siRNA inhibited endothelial cell growth. Mechanistic investigation showed that O-glycans were present on VEGFR2 and these structures were modulated by COSMC. Furthermore, VEGFR2 degradation...

Authors: Lee J Jr, Chen CH, Chen YH, Huang MJ, Huang J, Hung JS, Chen MT, Huang MC

Rap1 and Rap2 antagonistically control endothelial barrier resistance

...measurements of HUVEC monolayers and subsequent determination of the barrier resistance, which is a measure for the ease with which ions can pass cell junctions. In line with its well-established effect on cell-cell junctions, depletion of Rap1 decreases...

Authors: Pannekoek WJ, Linnemann JR, Brouwer PM, Bos JL, Rehmann H

Dopamine D2 receptor agonists inhibit lung cancer progression by reducing angiogenesis and tumor infiltrating myeloid derived suppressor cells

We sought to determine whether Dopamine D2 Receptor (D2R) agonists inhibit lung tumor progression and identify subpopulations of lung cancer patients that benefit most from D2R agonist therapy. We demonstrate D2R agonists abrogate lung tumor...

Authors: Hoeppner LH, Wang Y, Sharma A, Javeed N, Van Keulen VP, Wang E, Yang P, Roden AC, Peikert T, Molina...

SST Peer Reviewed

Melanoma cells homing to the brain: an in vitro model

We developed an in vitro contact through-feet blood brain barrier (BBB) model built using type IV collagen, rat astrocytes, and human umbilical vein endothelial cells (HUVECs) cocultured through Transwell porous polycarbonate membrane. The contact...

Authors: Rizzo A, Vasco C, Girgenti V, Fugnanesi V, Calatozzolo C2, Canazza A, Salmaggi A, Rivoltini L,...

In vitro reconstruction of branched tubular structures from lung epithelial cells in high cell concentration gradient environment

We have succeeded in developing hollow branching structure in vitro commonly observed in lung airway using primary lung airway epithelial cells. Cell concentration gradient is the key factor that determines production of the branching cellular...

Authors: Hagiwara M, Peng F, Ho CM

SST Peer Reviewed

Mammalian lysine histone demethylase KDM2A regulates E2F1-mediated gene transcription in breast cancer cells

...interfering RNAs demonstrated increased invasion and migration of breast cancer cells by suppressing a subset of matrix metalloproteinases (MMP-2, -9, -14 and -15), as seen by real-time PCR. HUVEC cells showed increased angiogenic tubule formation ability...

Authors: Rizwani W, Schaal C, Kunigal S, Coppola D, Chellappan S

Nuclear DNA sensor IFI16 as circulating protein in autoimmune diseases is a signal of damage that impairs endothelial cells through high-affinity membrane binding

...recombinant IFI16 binds these sites on HUVEC with dissociation constant of 2.7 nM, radioiodinated and unlabeled IFI16 compete for binding sites, with inhibition constant (Ki) of 14.43 nM and half maximal inhibitory concentration (IC50) of 67.88 nM; these data...

Authors: Gugliesi F, Bawadekar M, De Andrea M, Dell\'Oste V, Caneparo V, Tincani A, Gariglio M, Landolfo S.

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Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells

Engineering a 3D microfluidic culture platform for tumor-treating field application.

Shear stress-induced endothelial cell polarization in mediated by Rho and Rac but not Cdc42 or PI 3-kinase

COSMC is overexpressed in proliferating infantile hemangioma and enhances endothelial cell growth via VEGFR2

Rap1 and Rap2 antagonistically control endothelial barrier resistance

Dopamine D2 receptor agonists inhibit lung cancer progression by reducing angiogenesis and tumor infiltrating myeloid derived suppressor cells

Melanoma cells homing to the brain: an in vitro model

In vitro reconstruction of branched tubular structures from lung epithelial cells in high cell concentration gradient environment

Mammalian lysine histone demethylase KDM2A regulates E2F1-mediated gene transcription in breast cancer cells

Nuclear DNA sensor IFI16 as circulating protein in autoimmune diseases is a signal of damage that impairs endothelial cells through high-affinity membrane binding