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Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

An advanced intestine-on-chip model recreating epithelial 3D organotypic villus-like and crypt-like structures has been developed. The immunocompetent model includes Human Umbilical Vein Endothelial Cells (HUVEC), Caco-2 intestinal epithelial cells...

Authors: Adrian Feile , Valentin D Wegner , Martin Raasch , Alexander S Mosig

Role of Epac1, an Exchange Factor for Rap GTPases, in Endothelial Microtubule Dynamics and Barrier Function

...cell-cell interactions or Rap GTPase activation and was attributed to microtubule growth as assessed by time-lapse microscopy of HUVEC expressing fluorophore-linked microtubule plus-end marker end-binding protein 3. An intact microtubule network was required...

Authors: Sehrawat S, Cullere X, Patel S, Italiano J Jr, Mayadas TN

TEM4 is a junctional Rho GEF required for cell-cell adhesion, monolayer integrity and barrier function

...of cell-cell contact. The junctional localization of TEM4 was independent of actin binding. Depletion of endogenous TEM4 by shRNAs impaired Madin-Darby canine kidney (MDCK) and human umbilical vein endothelial cell (HUVEC) cell junctions, disrupted MDCK acini...

Authors: Ngok SP, Geyer R, Kourtidis A, Mitin N, Feathers R, Der C, Anastasiadis PZ

AILIM/ICOS signaling induces T-cell migration/polarization of memory/effector T-cells

...but also in fibroblast and endothelial cells in various organs. Here, we demonstrate that activated human T-cells migrate beneath TNF-alpha-treated HUVEC and display morphological polarization via AILIM/ICOS signaling. AILIM/ICOS stimulation, in the absence...

Authors: Okamoto N, Nukada Y, Tezuka K, Ohashi K, Mizuno K and Tsuji T

Lymphatic reprogramming of micro vascular endothelial cells by CEA-related Cell Adhesion Molecule-1 via interaction with VEGFR-3 and Prox1

...in FACS analyses while such effects are not observed in CEACAM1 overexpressing HUVECs. This effect of CEACAM1 is ceased when HDMECs are transfected with CEACAM1/y-- missing the tyrosine residues in its cytoplasmic domain. CEACAM1 overexpression in HDMECs...

Authors: Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E,...

Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay

...have used immunofluorescent staining to evaluate the expression of E-selectin on human microvascular endothelial cells from the skin (HDMEC) and human umbilical vein endothelial cells (HUVEC) exposed to various concentrations of LPS. In addition...

Authors: Unger RE, Peters K, Sartoris A, Freese C, Kirkpatrick CJ

Identification of an angiogenic factor that when mutated causes susceptibility to Klippel-Trenaunay syndrome

Angiogenic factors are critical to the initiation of angiogenesis and maintenance of the vascular network. Here we use human genetics as an approach to identify an angiogenic factor, VG5Q, and further define two genetic defects of VG5Q in patients...

Authors: Tian XL, Kadaba R, You SA, Liu M, Timur AA, Yang L, Chen Q, Szafranski P, Rao S, Wu L, Housman DE,...

Neuron-derived orphan receptor-1 (NOR-1) is induced by thrombin and mediates vascular endothelial cell growth

...of NOR-1 in thrombin-induced endothelial cell growth. RESULTS: Thrombin induced an early and transient up-regulation of NOR-1 in human umbilical vein endothelial cells (HUVEC). NOR-1 up-regulation by thrombin is dependent on multiple pathways, including...

Authors: Martorell L, MartÜnez-GonzÜ¡lez J, Crespo J, Calvayrac O, Badimon L

Angiogenic functionalisation of titanium surfaces using nano-anchored VEGF - an in vitro study

...of human umbilical vein endothelial cells (HUVECs) was significantly increased on the surfaces with specifically bound VEGF compared to the control surfaces and SAE Ti surfaces without VEGF. Moreover, the released conjugated VEGF exhibited biological...

Authors: Schliephake H, Strecker N, Förster A, Schwenzer B, Reichert J, Scharnweber D.

Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4

...carcinoma (Caco-2), but not in primary cultured normal cells, such as human brain microvascular endothelial cells (HBMECs); human umbilical vein endothelial cells (HUVECs) and human normal hepatocytes (hHeps™), with one exception (human cardiac fibloblasts...

Authors: Chida J, Araki H, Maeda Y

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