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How many mycoplasma species exist? Which are the usual suspects in cell culture?

More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale,...

Do we know the severity of the asthma or the genotype of the cystic fibrosis donors?

Generally, information on the severity of asthma is not available. For our diseased cells from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...

Do I need a special media or protocol for culturing the Clonetics™ and Poietics™ Diseased Cells?

No, all of our diseased cells are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart cells.

What are the benefits of using agarose for protein gel electrophoresis?

Protein electrophoresis in agarose gels is an alternative approach to using polyacrylamide gels and provides several benefits: - Separate high molecular weight proteins (>600 kDa)- Easy to prepare and handle- Efficient recovery of proteins-...

Can the ProSieve™ Molecular Weight Markers be used on native gels?

No, they are suitable for use on denaturing gels only.

Can an alternate to the nitrocellulose be used as an extracellular matrix when culturing rat cardiac myocytes?

Lonza’s rat cardiac myocytes can also be cultured on fibronectin or laminin. Nitrocellulose was selected as the recommended substrate for its convenience and ease of use.

I am using your ProSieve™ Color Protein Markers and the molecular weights (MW) of my protein of interest is not what I expected when compared to the ProSieve™ Markers. Why is this?

The proteins in our ProSieve™ Color Markers are made of proteins that are individually prestained using colored dyes.The level of dye bound can affect the migration of the protein and this can vary slightly lot to lot. Therefore, it is important to...

How long can Lonzas rat cardiac myocytes survive in culture?

Lonza has observed these cells up to 59 days in culture and even at that time, the cells displayed beating in syncytium. It is, however, possible that unseen physiological changes may occur to the cells during this time. As these cells are a...

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay should I use matched reagents, lysate/CSE?

You need to use matched reagents in order to comply with FDA requirements for endotoxin testing. Each Lonza LAL lot is tested for functionality using United States Reference Standard EC-7. We then match this LAL lot to a lot of our Control...

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