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What are the advantages of the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection assays over the rabbit test?

Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...

How should I properly heat my Lonza QCL-1000™ Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked...

How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...

Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?

Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...

How should I dispose of the FlashGel® Cassettes?

Dispose of the cassettes the same way you dispose of ethidium bromide stained gels. Follow state and local guidelines for disposal of these materials.

Can I cast my own gels to run on the FlashGel® Dock?

No. These cassettes are unique and proprietary. In order to use this system a special buffer, stain, and cassette are required.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

For cultivation of Smooth Muscle Cells - Can the insulin be left out of the SmGM™-2 Media?

Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...

Are the human mesenchymal stem cells (PT-2501) more prone to become differentiated down one lineage over the other?

MSCs are prone to differentiate down an adipogenic lineage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...

What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem cells?

Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...

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