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Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...

How does Lonza test for quality of the diseased airway cells?

Diseased Bronchial Epithelial Cells are characterized by morphology through serial passage and some are tested for differentiation using B-ALI™ Differentiation protocols, these are tested for TEER levels, ciliogenesis, and mucin production. Diseased...

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as for normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2...

What age are the mice and rats that your Clonetics™ Mouse and Rat Astrocytes are isolated from?

They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.

What donor characteristics are available for the diseased airway cells?

Most Clonetics™ Primary Cells have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support - if this...

Do I need to use a gelatin, fibronectin, collagen, or Matrigel™ coating when culturing my Clonetics™ Cells?

Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...

Your instructions for culturing of Clonetics™ Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead of...

What species of rat are the Clonetics™ Rat Astrocytes isolated from?

The rats are Sprague-Dawley.

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

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