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514 results sorted by
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Mycoplasmas belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "Mycoplasma" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct Mycoplasma...
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The rats are Sprague-Dawley and the mice are CD1 or C57.
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A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).
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No, the neurons do not proliferate.
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No. SDS has a negative influence on Adenylate Kinase (AK) activity. SDS is a cationic detergent, it is a very powerful protein denaturing agent, you will be aware of its use in SDS-PAGE for those reasons. ToxiLight™ works by measuring the activity of...
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The cells are cryopreserved at passage 0.
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A
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For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...
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There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...
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P
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We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...
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Yes. The cells do expand in culture.
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