Hepatocyte, human

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Human hepatocytes (either freshly isolated or cryopreserved)

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
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Research Area:
Cancer Research/Cell Biology
Toxicology
Cell Characteristics:
Adherent

Recommended Media

Hepatocyte Culture Medium

HCM is  a serum-free medium for maintenance of cultures prior to and during experimental procedures.
It is the preferred medium when working with cryopreserved human and animal hepatocytes.

Hepatocyte Maintenance Medium

HMM is a serum-free medium that may be used to maintain fresh plated human and animal hepatocytes in culture during testing.

Hepatocyte Plating Medium (MP)

Hepatic media for the plating of fresh and cryopreserved, human and animal, primary hepatocytes. The medium consists of a basal Medium with a supplement.

MP100: Hepatocyte Plating Medium w/Supplement (MP100-1 + MP100-2), 100ml
MP250: : Hepatocyte Plating Medium w/Supplement (MP250-1 + MP250-2), 250 ml

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-EX-147 1e5 54% 69% Plasmid (general) 1 µg 20 µl 96-well
P3 96-EX-147 1e5 54% 59% Plasmid (general) 1 µg 20 µl 96-well
P3 EX-147 5e5 68% ±11 53% ±5 Plasmid (general) 5 µg 100 µl 4D X-Unit
P3 DS-150 5e5 84% ±5 77% ±4 mRNA 5 µg 100 µl 4D X-Unit

Citations (83)

Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform
Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media 
Authors:
Christine Yee , Yik Lung Chan , Robert Utama , Marie Besnier , Martin Engel , Lisa Belfiore  
In:
STAR Protoc (2024) 5: 103234 
Uncovering the mechanism of troglitazone-mediated idiosyncratic drug-induced liver injury with individual-centric models
Categories:
Primary Cells and Media 
Authors:
Salomé Roux , Sara Cherradi , Hong Tuan Duong  
In:
Arch Toxicol (2024) 10: 1007 
Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes
Categories:
Primary Cells and Media 
Authors:
Yang Yuan , Vedant V Bodke , Christine Lin , Shang Gao , Jalees Rehman , Jisu Li , Salman R Khetani  
In:
Hepatology (2024) 8: e0506 
Induction of steatosis in primary human hepatocytes recapitulates key pathophysiological aspects of metabolic dysfunction-associated steatotic liver disease
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Yun Kwon , Pascal Gottmann , Surui Wang , Joel Tissink , Karsten Motzler , Revathi Sekar , Wiebke Albrecht , Cristina Cadenas , Jan G Hengstler , Annette Schürmann , Anja Zeigerer  
In:
J Clin Transl Hepatol (2024) 24: 02347 
Modeling the Effects of Protracted Cosmic Radiation in a Human Organ-on-Chip Platform
Categories:
3D Cell Culture  
Authors:
Daniel Naveed Tavakol , Trevor R Nash , Youngbin Kim  Pamela L Graney , Martin Liberman , Sharon Fleischer , Roberta I Lock , Aaron O'Donnell , Leah Andrews , Derek Ning , Keith Yeager , Andrew Harken , Naresh Deoli , Sally A Amundson , Guy Garty , Kam W Leong , David J Brenner , Gordana Vunjak-Novakovic  
In:
(2024) :  
Mapping of mitogen and metabolic sensitivity in organoids defines requirements for human hepatocyte growth
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Delilah Hendriks , Benedetta Artegiani , Thanasis Margaritis , Iris Zoutendijk , Susana Chuva de Sousa Lopes , Hans Clevers 
In:
Nature (2024) 15: 4034 
Efficient expansion and CRISPR-Cas9-mediated gene correction of patient-derived hepatocytes for treatment of inherited liver diseases
Categories:
Transfection 
Authors:
Kun Zhang , Ping Wan , Liren Wang , Zhen Wang , Fangzhi Tan , Jie Li , Xiaolong Ma , Jin Cen , Xiang Yuan , Yang Liu , Zhen Sun , Xi Cheng, Yuanhua Liu, Xuhao Liu, Jiazhi Hu , Guisheng Zhong, Dali Li , Qiang Xia , Lijian Hui  
In:
Cell Stem Cell (2024) 24: 00177-2 
Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research
Categories:
3D Cell Culture , Serum-free and Speciality Media 
Authors:
Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi 
In:
Scientific Reports (2024) 14: 1 
Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research
Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media 
Authors:
Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi 
In:
Scientific Reports (2024) 14: 1 
The role of sinusoidal endothelial cells and TIMP1 in the regulation of fibrosis in a novel human liver 3D NASH model
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
van Riet, Sander; Julien, Anais; Atanasov, Andrea Nordling, Åsa; Ingelman-Sundberg, Magnus 
In:
Hepatology (2024) 8: e0374. 
In vitro characterization of 3D culture-based differentiation of human liver stem cells
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Marta Tapparo , Gabriele Saccu , Chiara Pasquino , Valentina Fonsato , Claudio Medana , Valentina Schiavo , Enrica Mecarelli , Monica Maccagno , Lorenzo Silengo , Stefania Bruno , Giovanni Camussi , Maria Beatriz Herrera Sanchez  
In:
Frontiers in Pharmacology (2024) 12: 1352013 
Mechanisms of 5-HT receptor antagonists in the regulation of fibrosis in a 3D human liver spheroid model
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Sara Redenšek Trampuž, Sander van Riet, Åsa Nordling & Magnus Ingelman-Sundberg  
In:
Scientific Reports (2024) 14: 1396 
Chemical manipulation of an activation/inhibition switch in the nuclear receptor PXR
Categories:
 
Authors:
Efren Garcia-Maldonado , Andrew D Huber , Sergio C Chai , Stanley Nithianantham , Yongtao Li , Jing Wu , Shyaron Poudel , Darcie J Miller , Jayaraman Seetharaman , Taosheng Chen  
In:
Nature (2024) 15: 4054 
Establishment of a cell culture platform for human liver organoids and its application for lipid metabolism research
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Ayane Kuboyama-Sasaki , Yu Takahashi , Chen Xia , Kahori Hiro , Tsuyoshi Kobayashi , Hideki Ohdan , Makoto Shimizu , Yoshio Yamauchi , Hiroshi Kiyono , Ryuichiro Sato  
In:
Biotechnology Journal (2024) 19: 1 
Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers
Categories:
Primary Cells and Media 
Authors:
Xiao Liu , Kevin Lam , Huayi Zhao , Sadatsugu Sakane , Hyun Young Kim , Alvaro Eguileor , Karin Diggle , Shuai Wu , Raquel Carvalho Gontijo Weber , Pejman Soroosh , Mojgan Hosseini , Kristin Mekeel , David A Brenner , Tatiana Kisseleva  
In:
STAR Protoc (2023) 4: 3 
Elucidation of the liver pathophysiology of COVID-19 patients using liver-on-a-chips
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Sayaka Deguchi , Kaori Kosugi , Rina Hashimoto, Ayaka Sakamoto , Masaki Yamamoto, Rafal P Krol, Peter Gee , Ryosuke Negoro , Takeshi Noda , Takuya Yamamoto , Yu-Suke Torisawa , Miki Nagao , Kazuo Takayama  
In:
Proc Natl Acad Sci USA (2023) 2: 3 
COVID-19-related hyperglycemia is associated with infection of hepatocytes and stimulation of gluconeogenesis
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Ester A Barreto, Amanda S Cruz , Flavio P Veras, Ronaldo Martins , Rafaella S Bernardelli , Isadora M Paiva, Thais M Lima , Youvika Singh , Raphael C Guimarães , Samara Damasceno , Nayara Pereira , João Manoel Alves , Tiago T Gonçalves , Julia Forato , Stéfanie P Muraro, Gabriela F Souza , Sabrina Setembre Batah , José L Proenca-Modena , Marcelo A Mori , Fernando Q Cunha , Paulo Louzada-Junior, Thiago M Cunha , Helder I Nakaya , Alexandre Fabro , Renê D R de Oliveira , Eurico Arruda , Rosângela Réa , Álvaro Réa Neto , Miguel M Fernandes da Silva, Luiz Osório Leiria  
In:
Proc Natl Acad Sci USA (2023) 120: 21 
Liver three-dimensional cellular models for high-throughput chemical testing
Categories:
3D Cell Culture  
Authors:
Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia  
In:
Cell Rep (2023) 3: 100432 
Electroporation-mediated Delivery of Cas9Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes
Categories:
Transfection 
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de  
In:
CRISPR J (2022) :  
Application of in- vitro-cultured primary hepatocytes to evaluate species translatability and AAV transduction mechanisms of action
Categories:
Primary Cells and Media 
Authors:
Su Liu , Lisa Razon , Olivia Ritchie , Choong-Ryoul Sihn , Britta Handyside , Geoffrey Berguig , Jill Woloszynek , Lening Zhang , Paul Batty , David Lillicrap , Vishal Agrawal , Christa Cortesio , Kahsay Gebretsadik , Hassibullah Akeefe , Peter Colosi , Benjamin Kim , Stuart Bunting , Sylvia Fong  
In:
Mol Ther Methods Clin Dev. (2022) 26: 61-71 
Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Benoit Cox, Patrick Barton , Reiner Class, Hannah Coxhead, Claude Delatour , Eric Gillent , Jamie Henshall  , Emre M. Isin, Lloyd King , Jean-Pierre Valentin 
In:
Biomaterials (2022) 7: 100054 
CYP450 drug inducibility in NAFLD via an in vitro hepatic model:Understanding drug-drug interactions in the fatty liver
Categories:
Bioassay  
Authors:
Camilo Rey-Bedon , Peony Banik , Aslihan Gokaltun, O Hofheinz , Martin L Yarmush , M Korkut Uygun , O Berk Usta  
In:
(2022) 146: 112377 
A 3D Human Liver Model of Nonalcoholic Steatohepatitis
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Marion Duriez , Agnes Jacquet , Lucile Hoet , Sandrine Roche , Marie-Dominique Bock , Corinne Rocher , Gilles Haussy , Xavier Vigé , Zsolt Bocskei , Tamara Slavnic , Valérie Martin , Jean-Claude Guillemot , Michel Didier , Aimo Kannt , Cécile Orsini , Vincent Mikol , Anne-Céline Le Fèvre  
In:
J Clin Transl Hepatol (2020) 8(4): 359-370 
Single-cell metabolic profiling reveals subgroups of primary human hepatocytes showing heterogeneous responses to drug challenge
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
E. Sanchez-Quant, M. L. Richter, M. Colomé-Tatché , C.P. Martinez-Jimenez 
In:
BioResearch Open Access (2020) 0: 0 
Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti 
In:
Cell Rep (2020) 1 (4): 100060 
Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance
Categories:
Primary Cells and Media 
Authors:
Toba Y, Deguchi S, Mimura N, Sakamoto A, Harada K, Hirata K, Takayama K, Mizuguchi H. 
In:
PLoS ONE (2020) 15: e0229654  
Functionality of Primary Hepatic Non-Parenchymal Cells in a 3D Spheroid Model and Contribution to Acetaminophen Hepatotoxicity
Categories:
3D Cell Culture , Bioassay  
Authors:
Catherine C Bell , Bhavik Chouhan , Linda C Andersson , Håkan Andersson , James W Dear , Dominic P Williams , Magnus Söderberg  
In:
Arch Toxicol (2020) 94: 1251 
Generation of hepatobiliary organoids from human induced pluripotent stem cells
Categories:
 
Authors:
Fenfang Wu , Di Wu , Yong Ren , Yuhua Huang , Bo Feng , Nan Zhao , Taotao Zhang , Xiaoni Chen , Shangwu Chen , Anlong Xu  
In:
J Clin Transl Hepatol (2019) 70: 1145 
A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum.
Categories:
Primary Cells and Media 
Authors:
Roth A, Maher SP, Conway AJ, Ubalee R, Chaumeau V, Andolina C, Kaba SA, Vantaux A, Bakowski MA, Thomson-Luque R, Adapa SR, Singh N, Barnes SJ, Cooper CA, Rouillier M, McNamara CW, Mikolajczak SA, Sather N, Witkowski B, Campo B, Kappe SHI, Lanar DE, Nosten F, Davidson S, Jiang RHY, Kyle DE, Adams JH. 
In:
Nature (2018) 9: 1 
Environmental Toxin Screening Using Human-Derived 3D Bioengineered Liver and Cardiac Organoids
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Forsythe SD, Devarasetty M, Shupe T, Bishop C, Atala A, Soker S, Skardal A 
In:
Frontiers in Public Health (2018) 6 (103):  
A Novel In Vitro Liver Cell Culture Flow System Allowing Long-Term Metabolism and Hepatotoxicity Studies
Categories:
Primary Cells and Media, 3D Cell Culture , Laboratory Instrumentation  
Authors:
Buesch S, Schroeder S, Bunger M, D'Souza T, Stosic M 
In:
Appl In Vitro Toxicol (2018) :  
Simulation of human plasma concentration-time profiles of the partial glucokinase activator PF-04937319 and its disproportionate N-demethylated metabolite using humanized chimeric mice and semi-physiological pharmacokinetic modeling
Categories:
Primary Cells and Media 
Authors:
Kamimura H1,2, Ito S1, Chijiwa H3, Okuzono T3, Ishiguro T3, Yamamoto Y3, Nishinoaki S3, Ninomiya SI1, Mitsui M4, Kalgutkar AS5, Yamazaki H4, Suemizu H2 
In:
Other (2017) 47(5): 382-393 
HCV Induces Telomerase Reverse Transcriptase, Increases Its Catalytic Activity, and Promotes Caspase Degradation in Infected Human Hepatocytes.
Categories:
Primary Cells and Media 
Authors:
Zhu Z, Tran H, Mathahs MM, Moninger TO, Schmidt WN 
In:
PLoS ONE (2017) 12(1): doi: 10.1371/journal.pone.0166853 
Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
Categories:
Primary Cells and Media 
Authors:
Feaver RE1, Cole BK1, Lawson MJ1, Hoang SA1, Marukian S1, Blackman BR2, Figler RA1, Sanyal AJ3, Wamhoff BR1, Dash A1 
In:
Other (2016) 1(20): e90954 
Higher Serum Alanine Transaminase Levels in Male Urokinase-Type Plasminogen Activator-Transgenic Mice Are Associated With Improved Engraftment of Hepatocytes but not Liver Sinusoidal Endothelial Cells.
Categories:
Primary Cells and Media 
Authors:
Fomin ME, Beyer AI, Publicover J, Lu K, Bakkour S, Simmons G, Muench MO 
In:
Cell Medicine (2016) 9(3): 117-125 
High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.
Categories:
 
Authors:
Shan J1, Logan DJ2, Root DE3, Carpenter AE3, Bhatia SN4 
In:
J Biomol Screen (2016) 21(9): 410-419 
A Cell-type-resolved Liver Proteome
Categories:
 
Authors:
Ding C1, Li Y2, Guo F3, Jiang Y3, Ying W3, Li D3, Yang D3, Xia X3, Liu W3, Zhao Y3, He Y4, Li X3, Sun W3, Liu Q3, Song L3, Zhen B3, Zhang P3, Qian X5, Qin J6, He F7. 
In:
Mol Cell Proteomics (2016) 15(10): 3190-3202 
Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes.
Categories:
Primary Cells and Media 
Authors:
Davidson MD1,2, Ballinger KR3, Khetani SR1,2,3 
In:
Scientific Reports (2016) 6: 28178 
Long-term maintenance of a microfluidic 3D human liver sinusoid
Categories:
Primary Cells and Media 
Authors:
Prodanov L1, Jindal R1, Bale SS1, Hegde M1, McCarty WJ1, Golberg I1, Bhushan A1, Yarmush ML2,3, Usta OB4 
In:
Biotechnol Bioeng (2016) 113(1): 241-6 
Downregulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine: Implication in OATP-Mediated Drug-Drug Interactions
Categories:
Primary Cells and Media 
Authors:
Alam K, Pahwa S, Wang X, Zhang P, Ding K, Abuznait AH, Li L, Yue W 
In:
Mol Pharmacol (2016) 13(3): 839-51 
Long-term culture and expansion of primary human hepatocytes
Categories:
 
Authors:
Levy G, Bomze D, Heinz S, Ramachandran SD, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y. 
In:
Nat Biotechnol (2015) 33(12): 1264-1271 
P2X3 purinergic receptor overexpression is associated with poor recurrence-free survival in hepatocellular carcinoma patients.
Categories:
Primary Cells and Media 
Authors:
Maynard JP1,2, Lee JS3, Sohn BH3, Yu X4, Lopez-Terrada D5, Finegold MJ2,5, Goss JA2,6, Thevananther S1,2 
In:
OncoTarget (2015) 6(38): 41162-79 
Acetaldehyde accelerates HCV-induced impairment of innate immunity by suppressing methylation reactions in liver cells
Categories:
Primary Cells and Media 
Authors:
Murali Ganesan,1,2 Jinjin Zhang,3 Tatiana Bronich,3 Larisa I. Poluektova,2,4 Terrence M. Donohue, Jr.,1,2 Dean J. Tuma,1,2 Kusum K. Kharbanda,1,2 and Natalia A. Osna1,2 
In:
Am J Physiol Gastrointest Liver Physiol (2015) 309: G566-G577 
Hormone and Drug-Mediated Modulation of Glucose Metabolism in a Microscale Model of the Human Liver.
Categories:
Primary Cells and Media 
Authors:
Davidson MD1, Lehrer M2, Khetani SR1,3 
In:
Tissue Eng (2015) 21(7): 716-725 
Microengineered liver tissues for drug testing
Categories:
Primary Cells and Media 
Authors:
Khetani SR1, Berger DR2, Ballinger KR3, Davidson MD2, Lin C2, Ware BR2 
In:
J Lab Automation (2015) 20(3): 216-250 
Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model.
Categories:
Primary Cells and Media 
Authors:
Bissig-Choisat B1, Wang L2, Legras X1, Saha PK3, Chen L1, Bell P2, Pankowicz FP4, Hill MC5, Barzi M1, Kettlun Leyton C1, Leung HC6, Kruse RL7, Himes RW8, Goss JA9, Wilson JM2, Chan L3, Lagor WR10, Bissig KD11 
In:
Nat Commun. (2015) 6(7339: 1038 
Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro
Categories:
Primary Cells and Media 
Authors:
Dumoulin PC, Trop SA, Ma J, Zhang H, Sherman MA, Levitskaya J 
In:
PLoS ONE (2015) 10(6): 0129623 
Safety assessment of potential food ingredients in canine hepatocytes
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang LW, Koci J, Jeffery B2, Riviere JE, Monteiro-Riviere NA 
In:
Food Chem Toxicol (2015) 78: 105-15 
Differential activation of human constitutive androstane receptor and its SV23 and SV24 splice variants by rilpivirine and etravirine
Categories:
 
Authors:
Sharma D1, Lau AJ, Sherman MA, Chang TK. 
In:
Br J Pharmacol (2015) 172(5): 1263-1276 
3D Cultivation Techniques for Primary Human Hepatocytes.
Categories:
 
Authors:
Bachmann A1, Moll M2, Gottwald E3, Nies C4, Zantl R5, Wagner H6, Burkhardt B7, Sánchez JJ8, Ladurner R9, Thasler W10, Damm G11, Nussler AK12. 
In:
Other (2015) 4(1): 64-83 
Spontaneous dormancy of metastatic breast cancer cells in an all human liver microphysiologic system
Categories:
 
Authors:
Wheeler SE1, Clark AM1, Taylor DP2, Young CL3, Pillai VC4, Stolz DB5, Venkataramanan R6, Lauffenburger D3, Griffith L3, Wells A7. 
In:
Br J Cancer (2014) 111(12): 2342-2350 
MicroRNA-mediated multi-tissue detargeting of oncolytic measles virus.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Baertsch M1, Leber MF, Bossow S, Singh M, Engeland CE, Albert J, Grossardt C, Jäger D, von Kalle C, Ungerechts G. 
In:
Cancer Gene Ther (2014) 21(9): 373-80 
Hepatocytes buried in the cirrhotic livers of patients with biliary atresia proliferate and function in the livers of urokinase-type plasminogen activator-NOG mice.
Categories:
Primary Cells and Media 
Authors:
Suemizu H, Nakamura K, Kawai K, Higuchi Y, Kasahara M, Fujimoto J, Tanoue A, Nakamura M. 
In:
Liver Trans (2014) 20(9): 1127-37 
Integrated Analysis of Rifampicin-induced MicroRNA and Gene Expression Changes in Human Hepatocytes
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takahashi K, Tatsumi N, Fukami T, Yokoi T, Nakajima M. 
In:
Drug Metab Pharmacokinet (2014) 29(4): 333-40 
Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chida J, Araki H, Maeda Y 
In:
Cancer Cell (2014) 14: 56 
Aneuploidy is permissive for hepatocyte-like cell differentiation from human induced pluripotent stem cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Noto FK, Determan MR, Cai J, Cayo MA, Mallanna SK, Duncan SA 
In:
BMC Res Notes (2014) 7: 437 
The mitogen-activated protein (MAP) kinases p38 and extracellular signal-regulated kinase (ERK) are involved in hepatocyte-mediated phenotypic switching in prostate cancer cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ma B, Wells A. 
In:
J Biol Chem (2014) 289(16): 11153-61 
Statins impair glucose uptake in human cells.
Categories:
Primary Cells and Media 
Authors:
Nowis D, Malenda A, Furs K, Oleszczak B, Sadowski R, Chlebowska J, Firczuk M, Bujnicki JM, Staruch AD, Zagozdzon R, Glodkowska-Mrowka E, Szablewski L, Golab J 
In:
Other (2014) 2(1): ePub 
Evaluation of the use of imaging parameters for the detection of compound-induced hepatotoxicity in 384-well cultures of HepG2 cells and cryopreserved primary human hepatocytes
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Garside H, Marcoe KF, Chesnut-Speelman J, Foster AJ, Muthas D, Kenna JG, Warrior U, Bowes J, Baumgartner J 
In:
Toxicol In Vitro (2014) ;28(2): 171-81 
Hepcidin induction by pathogens and pathogen-derived molecules is strongly dependent on interleukin-6
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rodriguez R1, Jung CL, Gabayan V, Deng JC, Ganz T, Nemeth E, Bulut Y. 
In:
Infect Immun (2014) 82(2): 745-52 
Evaluation of various static and dynamic modeling methods to predict clinical CYP3A induction using in vitro CYP3A4 mRNA induction data
Categories:
 
Authors:
Einolf HJ1, Chen L2, Fahmi OA3, Gibson CR4, Obach RS3, Shebley M5, Silva J6, Sinz MW7, Unadkat JD8, Zhang L9, Zhao P9 
In:
Other (2014) 95(2): 179-188 
Elaidate, an 18-carbon trans-monoenoic fatty acid, inhibits ß-oxidation in human peripheral blood macrophages
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zacherl JR, Mihalik SJ, Chace DH, Christensen TC, Robinson LJ, Blair HC. 
In:
J Cell Biol (2014) 115(1): 62-70 
Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.
Categories:
 
Authors:
Godoy P1, Hewitt NJ, Albrecht U, Andersen ME, Ansari N, Bhattacharya S, Bode JG, Bolleyn J, Borner C, Böttger J, Braeuning A, Budinsky RA, Burkhardt B, Cameron NR, Camussi G, Cho CS, Choi YJ, Craig Rowlands J, Dahmen U, Damm G, Dirsch O, Donato MT, Dong J, Dooley S, Drasdo D, Eakins R, Ferreira KS, Fonsato V, Fraczek J, Gebhardt R, Gibson A, Glanemann M, Goldring CE, Gómez-Lechón MJ, Groothuis GM, Gustavsson L, Guyot C, Hallifax D, Hammad S, Hayward A, Häussinger D, Hellerbrand C, Hewitt P, Hoehme S, Holzhütter HG, Houston JB, Hrach J, Ito K, Jaeschke H, Keitel V, Kelm JM, Kevin Park B, Kordes C, Kullak-Ublick GA, LeCluyse EL, Lu P, Luebke-Wheeler J, Lutz A, Maltman DJ, Matz-Soja M, McMullen P, Merfort I, Messner S, Meyer C, Mwinyi J, Naisbitt DJ, Nussler AK, Olinga P, Pampaloni F, Pi J, Pluta L, Przyborski SA, Ramachandran A, Rogiers V, Rowe C, Schelcher C, Schmich K, Schwarz M, Singh B, Stelzer EH, Stieger B, Stöber R, Sugiyama Y, Tetta C, Thasler WE, Vanhaecke T, Vinken M, Weiss TS, Widera A, Woods CG, Xu JJ, Yarborough KM, Hengstler JG. 
In:
Other (2013) 87(8): 1315-1530 
Agonism of human pregnane X receptor by rilpivirine and etravirine: comparison with first generation non-nucleoside reverse transcriptase inhibitors.
Categories:
Primary Cells and Media 
Authors:
Sharma D1, Lau AJ, Sherman MA, Chang TK 
In:
Biochem Pharmacol (2013) 85(11): 1700-11 
ADME of biologics-what have we learned from small molecules?
Categories:
 
Authors:
Prueksaritanont T1, Tang C. 
In:
Other (2012) 14(3): 410-419 
Adenoviral vector driven by a minimal Rad51 promoter is selective for p53-deficient tumor cells.
Categories:
Primary Cells and Media 
Authors:
Fong V, Osterbur M, Capella C, Kim YE, Hine C, Gorbunova V, Seluanov A, Dewhurst S 
In:
PLoS ONE (2011) 6(12): e28714 
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors.
Categories:
Transfection 
Authors:
Motoyama H, Ogawa S, Kubo A, Miwa S, Nakayama J, Tagawa Y, Miyagawa S. 
In:
Biochem Biophys Res Commun (2009) 385 (1): 123-8 
Culture of bovine hepatocytes: a non-perfusion technique for cell isolation
Categories:
 
Authors:
Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O. 
In:
Cytotechnology (2006) 51(2): 51-56 
CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection.
Categories:
 
Authors:
Crespi CL1, Chang TK, Waxman DJ. 
In:
Methods Mol Biol (2006) 320: 115-119 
Metabolism-based drug-drug interactions: what determines individual variability in cytochrome P450 induction?
Categories:
 
Authors:
Tang C1, Lin JH, Lu AY 
In:
Drug Metabolism and Disposition (2005) 33(5): 603-613 
Human hepatocyte depletion in the presence of HIV-1 infection in dual reconstituted humanized mice
Categories:
Primary Cells and Media 
Authors:
Dagur RS1, Wang W2, Cheng Y2, Makarov E2, Ganesan M3,4, Suemizu H5, Gebhart CL6, Gorantla S2, Osna N3,4, Poluektova LY1. 
In:
Open Biology () 2:  
Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro
Categories:
Primary Cells and Media 
Authors:
Dumoulin PC1, Trop SA1, Ma J1, Zhang H1, Sherman MA2, Levitskaya J1 
In:
PLoS ONE () :  
Elevated mitochondrial activity distinguishes fibrogenic hepatic stellate cells and sensitizes for selective inhibition by mitotropic doxorubicin
Categories:
 
Authors:
Gajendiran P1, Vega LI1, Itoh K2, Sesaki H2, Vakili MR3, Lavasanifar A3, Hong K1, Mezey E4, Ganapathy-Kanniappan S1. 
In:
J Cell Mol Med () 4: 2210-2219 
Prolonged cenicriviroc therapy reduces hepatic fibrosis despite steatohepatitis in a diet-induced mouse model of nonalcoholic steatohepatitis.
Categories:
Primary Cells and Media 
Authors:
Kruger AJ1, Fuchs BC2, Masia R3, Holmes JA1,4, Salloum S1, Sojoodi M2, Ferreira DS5, Rutledge SM6, Caravan P5, Alatrakchi N1, Vig P7, Lefebvre E7, Chung RT1. 
In:
Other () 5: 529-545 
FGF23 and Fetuin-A Interaction in the Liver and in the Circulation.
Categories:
Primary Cells and Media 
Authors:
Mattinzoli D1, Ikehata M1, Tsugawa K1, Alfieri CM1,2, Dongiovanni P3, Trombetta E4, Valenti L5,6, Puliti A7,8, Lazzari L9, Messa P1,2,6. 
In:
Other () 6: 586-598 
Prediction and Quantification of Hepatic Transporter-Mediated Uptake of Pitavastatin Utilizing a Combination of the Relative Activity Factor Approach and Mechanistic Modeling.
Categories:
Primary Cells and Media 
Authors:
Mitra P1, Weinheimer S2, Michalewicz M2, Taub ME2. 
In:
Drug Metabolism and Disposition () 7: 953-963 
Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX.
Categories:
Primary Cells and Media 
Authors:
Ramaswamy S1, Tonnu N1, Menon T2, Lewis BM1, Green KT3, Wampler D4, Monahan PE5, Verma IM6. 
In:
Cell Rep () 5: 1565-1580 
In vitro assessment of nutraceutical compounds and novel nutraceutical formulations in a liver-steatosis-based model.
Categories:
Primary Cells and Media 
Authors:
Stellavato A1, Pirozzi AVA1, de Novellis F1, Scognamiglio I1, Vassallo V1, Giori AM2, De Rosa M1, Schiraldi C3. 
In:
Lipids in Health and Disease () :  
Microphysiological Systems to Assess Nonclinical Toxicity.
Categories:
Primary Cells and Media 
Authors:
Van Ness KP1, Chang SY2, Weber EJ1, Zumpano D3, Eaton DL2, Kelly EJ1 
In:
Curr Protoc Immunol () 14: 1-14 
Human pluripotent stem cell derived HLC transcriptome data enables molecular dissection of hepatogenesis.
Categories:
Primary Cells and Media 
Authors:
Wruck W1, Adjaye J1 
In:
() 5: 180035 
Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro
Categories:
Primary Cells and Media 
Authors:
Dumoulin PC, Trop SA,  Ma J, Zhang H, Sherman MA, Levitskaya J 
In:
PLoS ONE () 10: 6 
Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies
Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media 
Authors:
Emi Sano, Sayaka Deguchi , Naoki Matsuoka , Masahiro Tsuda, Mengyang Wang , Kaori Kosugi  Chihiro Mori , Keisuke Yagi , Aya Wada , Shinsuke Yamasaki , Tsuyoshi Kawai , Masahide Yodogawa, Hiroyuki Mizuguchi , Norihito Nakazawa , Fumiyoshi Yamashita , Yu-Suke Torisawa , Kazuo Takayama  
In:
ACS Nano () 6: 24859 
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