A Cell-type-resolved Liver Proteome

Ding C1, Li Y2, Guo F3, Jiang Y3, Ying W3, Li D3, Yang D3, Xia X3, Liu W3, Zhao Y3, He Y4, Li X3, Sun W3, Liu Q3, Song L3, Zhen B3, Zhang P3, Qian X5, Qin J6, He F7.
Source: Mol Cell Proteomics
Publication Date: (2016)
Issue: 15(10): 3190-3202
Cells used in publication:
Hepatocyte, mouse
Species: mouse
Tissue Origin: liver
Hepatocyte, rat
Species: rat
Tissue Origin: liver
Hepatocyte, human
Species: human
Tissue Origin: liver
Hepatocyte, Cynomolgus
Species: monkey
Tissue Origin: liver
Hepatocyte, Rhesus
Species: monkey
Tissue Origin: liver
Hepatocyte, canine
Species: canine
Tissue Origin:
Parenchymatous organs consist of multiple cell types, primarily defined as parenchymal cells (PCs) and nonparenchymal cells (NPCs). The cellular characteristics of these organs are not well understood. Proteomic studies facilitate the resolution of the molecular details of different cell types in organs. These studies have significantly extended our knowledge about organogenesis and organ cellular composition. Here, we present an atlas of the cell-type-resolved liver proteome. In-depth proteomics identified 6000 to 8000 gene products (GPs) for each cell type and a total of 10,075 GPs for four cell types. This data set revealed features of the cellular composition of the liver: (1) hepatocytes (PCs) express the least GPs, have a unique but highly homogenous proteome pattern, and execute fundamental liver functions; (2) the division of labor among PCs and NPCs follows a model in which PCs make the main components of pathways, but NPCs trigger the pathways; and (3) crosstalk among NPCs and PCs maintains the PC phenotype. This study presents the liver proteome at cell resolution, serving as a research model for dissecting the cell type constitution and organ features at the molecular level.