Hepatocyte, mouse


Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Toxicology
Cell Characteristics:
Suspension

Recommended Media

HCM is  a serum-free medium for maintenance of cultures prior to and during experimental procedures.
It is the preferred medium when working with cryopreserved human and animal hepatocytes.

HMM is a serum-free medium that may be used to maintain fresh plated human and animal hepatocytes in culture during testing.

Hepatic media for the plating of fresh and cryopreserved, human and animal, primary hepatocytes. The medium consists of a basal Medium with a supplement.

MP100: Hepatocyte Plating Medium w/Supplement (MP100-1 + MP100-2), 100ml
MP250: : Hepatocyte Plating Medium w/Supplement (MP250-1 + MP250-2), 250 ml

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Hepatocyte, mouse/rat T-028 3e5 54% ±8 80% ±7 Plasmid (general) 4 µg 100 µl I/II/2b

Citations (17)

Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media 
Authors:
Lulu Zhang , Xunting Wang , Xuelian Yang , Yijia Chi , Yihang Chu , Yi Zhang , Yufan Gong , Fei Wang , Qian Zhao , Dongxin Zhao  
In:
Open Biology (2024) 13: 9 
Categories:
 
Authors:
Efren Garcia-Maldonado , Andrew D Huber , Sergio C Chai , Stanley Nithianantham , Yongtao Li , Jing Wu , Shyaron Poudel , Darcie J Miller , Jayaraman Seetharaman , Taosheng Chen  
In:
Nature (2024) 15: 4054 
Categories:
Transfection 
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de  
In:
CRISPR J (2022) :  
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Cell Lines and Primary Cancer Cells 
Authors:
Shin-Wei C hen, Misao Himeno, Yuta Koui, Masaya Sugiyama, Hironori Nishitsuji, Masashi Mizokami, Kunitada Shimotohno, Atsushi Miyajima & Taketomo Kido 
In:
Scientific Reports (2020) 10: 14349 
Categories:
 
Authors:
Ding C1, Li Y2, Guo F3, Jiang Y3, Ying W3, Li D3, Yang D3, Xia X3, Liu W3, Zhao Y3, He Y4, Li X3, Sun W3, Liu Q3, Song L3, Zhen B3, Zhang P3, Qian X5, Qin J6, He F7. 
In:
Mol Cell Proteomics (2016) 15(10): 3190-3202 
Categories:
Primary Cells and Media 
Authors:
Khetani SR1, Berger DR2, Ballinger KR3, Davidson MD2, Lin C2, Ware BR2 
In:
J Lab Automation (2015) 20(3): 216-250 
Categories:
 
Authors:
Einolf HJ1, Chen L2, Fahmi OA3, Gibson CR4, Obach RS3, Shebley M5, Silva J6, Sinz MW7, Unadkat JD8, Zhang L9, Zhao P9 
In:
Other (2014) 95(2): 179-188 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jenkins Y, Sun TQ, Markovtsov V, Foretz M, Li W, Nguyen H, Li Yi, Pan A, Uy G, Gross L, Baltgalvis K, Yung SL, Gururaja T, Kinoshita T, Owyang A, Smith IJ, McCaughey K, White K, Godinez G, Alcantara R 
In:
PLoS ONE (2013) 8(12): ePub 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takayama K1, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, Hayakawa T, Kawabata K, Mizuguchi H 
In:
Stem Cell Reports (2013) 1(4): 322-35 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gao L, Utsumi T, Tashiro K, Liu B, Zhang D, Swenson ES, Iwakiri Y. 
In:
Hepatology (2013) 57(5): 1992-2003 
Categories:
 
Authors:
Prueksaritanont T1, Tang C. 
In:
Other (2012) 14(3): 410-419 
Categories:
 
Authors:
Martinez SM1, Bradford BU, Soldatow VY, Kosyk O, Sandot A, Witek R, Kaiser R, Stewart T, Amaral K, Freeman K, Black C, LeCluyse EL, Ferguson SS, Rusyn I 
In:
Toxicol Appl Pharmacol (2010) 249(3): 208-216 
Categories:
Transfection 
Authors:
Müller-Hartmann H, Faust N, Kazinski M, Kretzschmar T 
In:
Exp Opin Drug Discov (2007) 2(11): 1453-1465 
Categories:
 
Authors:
Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O. 
In:
Cytotechnology (2006) 51(2): 51-56 
Categories:
 
Authors:
Crespi CL1, Chang TK, Waxman DJ. 
In:
Methods Mol Biol (2006) 320: 115-119 
Categories:
 
Authors:
Tang C1, Lin JH, Lu AY 
In:
Drug Metabolism and Disposition (2005) 33(5): 603-613 
Categories:
Transfection 
Authors:
Chen NK, Sivalingam J, Tan SY, Kon OL 
In:
Gene Ther (2005) 12(8): 655-667 
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