Hepatocyte, mouse

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Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Toxicology
Cell Characteristics:
Suspension

Recommended Media

Hepatocyte Culture Medium

HCM is  a serum-free medium for maintenance of cultures prior to and during experimental procedures.
It is the preferred medium when working with cryopreserved human and animal hepatocytes.

Hepatocyte Maintenance Medium

HMM is a serum-free medium that may be used to maintain fresh plated human and animal hepatocytes in culture during testing.

Hepatocyte Plating Medium (MP)

Hepatic media for the plating of fresh and cryopreserved, human and animal, primary hepatocytes. The medium consists of a basal Medium with a supplement.

MP100: Hepatocyte Plating Medium w/Supplement (MP100-1 + MP100-2), 100ml
MP250: : Hepatocyte Plating Medium w/Supplement (MP250-1 + MP250-2), 250 ml

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Hepatocyte, mouse/rat T-028 3e5 54% ±8 80% ±7 Plasmid (general) 4 µg 100 µl I/II/2b

Citations (15)

Electroporation-mediated Delivery of Cas9Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes
Categories:
Transfection 
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de  
In:
CRISPR J (2022) :  
Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Cell Lines and Primary Cancer Cells 
Authors:
Shin-Wei C hen, Misao Himeno, Yuta Koui, Masaya Sugiyama, Hironori Nishitsuji, Masashi Mizokami, Kunitada Shimotohno, Atsushi Miyajima & Taketomo Kido 
In:
Scientific Reports (2020) 10: 14349 
A Cell-type-resolved Liver Proteome
Categories:
 
Authors:
Ding C1, Li Y2, Guo F3, Jiang Y3, Ying W3, Li D3, Yang D3, Xia X3, Liu W3, Zhao Y3, He Y4, Li X3, Sun W3, Liu Q3, Song L3, Zhen B3, Zhang P3, Qian X5, Qin J6, He F7. 
In:
Mol Cell Proteomics (2016) 15(10): 3190-3202 
Microengineered liver tissues for drug testing
Categories:
Primary Cells and Media 
Authors:
Khetani SR1, Berger DR2, Ballinger KR3, Davidson MD2, Lin C2, Ware BR2 
In:
J Lab Automation (2015) 20(3): 216-250 
Evaluation of various static and dynamic modeling methods to predict clinical CYP3A induction using in vitro CYP3A4 mRNA induction data
Categories:
 
Authors:
Einolf HJ1, Chen L2, Fahmi OA3, Gibson CR4, Obach RS3, Shebley M5, Silva J6, Sinz MW7, Unadkat JD8, Zhang L9, Zhao P9 
In:
Other (2014) 95(2): 179-188 
AMPK Activation through Mitochondrial Regulation Results in Increased Substrate Oxidation and Improved Metabolic Parameters in Models of Diabetes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jenkins Y, Sun TQ, Markovtsov V, Foretz M, Li W, Nguyen H, Li Yi, Pan A, Uy G, Gross L, Baltgalvis K, Yung SL, Gururaja T, Kinoshita T, Owyang A, Smith IJ, McCaughey K, White K, Godinez G, Alcantara R 
In:
PLoS ONE (2013) 8(12): ePub 
Long-Term Self-Renewal of Human ES/iPS-Derived Hepatoblast-like Cells on Human Laminin 111-Coated Dishes
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takayama K1, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, Hayakawa T, Kawabata K, Mizuguchi H 
In:
Stem Cell Reports (2013) 1(4): 322-35 
Reticulon 4B (Nogo-B) facilitates hepatocyte proliferation and liver regeneration in mice.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gao L, Utsumi T, Tashiro K, Liu B, Zhang D, Swenson ES, Iwakiri Y. 
In:
Hepatology (2013) 57(5): 1992-2003 
ADME of biologics-what have we learned from small molecules?
Categories:
 
Authors:
Prueksaritanont T1, Tang C. 
In:
Other (2012) 14(3): 410-419 
Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity
Categories:
 
Authors:
Martinez SM1, Bradford BU, Soldatow VY, Kosyk O, Sandot A, Witek R, Kaiser R, Stewart T, Amaral K, Freeman K, Black C, LeCluyse EL, Ferguson SS, Rusyn I 
In:
Toxicol Appl Pharmacol (2010) 249(3): 208-216 
High-throughput transfection and engineering of primary cells and cultured cell lines - an invaluable tool for research as well as drug development
Categories:
Transfection 
Authors:
Müller-Hartmann H, Faust N, Kazinski M, Kretzschmar T 
In:
Exp Opin Drug Discov (2007) 2(11): 1453-1465 
Culture of bovine hepatocytes: a non-perfusion technique for cell isolation
Categories:
 
Authors:
Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O. 
In:
Cytotechnology (2006) 51(2): 51-56 
CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection.
Categories:
 
Authors:
Crespi CL1, Chang TK, Waxman DJ. 
In:
Methods Mol Biol (2006) 320: 115-119 
Metabolism-based drug-drug interactions: what determines individual variability in cytochrome P450 induction?
Categories:
 
Authors:
Tang C1, Lin JH, Lu AY 
In:
Drug Metabolism and Disposition (2005) 33(5): 603-613 
Plasmid-electroporated primary hepatocytes acquire quasi-physiological secretion of human insulin and restore euglycemia in diabetic mice
Categories:
Transfection 
Authors:
Chen NK, Sivalingam J, Tan SY, Kon OL 
In:
Gene Ther (2005) 12(8): 655-667 
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