RAW 264.7

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Macrophage cell line established from a tumor induced by Abelson murine leukemia virus in male adult BALB/c mouse.

Cell Type:
Macrophage
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V D-032 2e6 62-68% 67-81% Plasmid (general) 2 µg 100 µl I/II/2b
SF 96-DS-136 4e5 51-69% 82-90% Plasmid (general) 0.4 µg 20 µl 96-well
SF

DS-136

 4e5 51-69% 82-90% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (60)

Gene Regulatory Network Inference of Immunoresponsive Gene 1 (IRG1) Identifies Interferon Regulatory Factor 1 (IRF1) as Its Transcriptional Regulator in Mammalian Macrophages.
Categories:
Transfection 
Authors:
Tallam A, Perumal TM, Antony PM, Jäger C, Fritz JV, Vallar L, Balling R, Del Sol A, Michelucci A. 
In:
PLoS ONE (2016) 11(2): 1-28 
FOXO1 mediates RANKL-induced osteoclast formation and activity.
Categories:
Primary Cells and Media 
Authors:
Wang Y, Dong G, Jeon HH, Elazizi M, La LB, Hameedaldeen A, Xiao E, Tian C, Alsadun S, Choi Y, Graves DT. 
In:
J Immunol (2015) 194(6): 2878-87 
Mitf induction by RANKL is critical for osteoclastogenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lu SY, Li M, Lin YL. 
In:
Mol Biol Cell (2010) 21(10): 1763-71 
Impaired micro-RNA pathways diminish osteoclast differentiation and function
Categories:
Bioassay  
Authors:
Sugatani T, Hruska KA. 
In:
J Biol Chem (2009) 284(7): 4667-78 
Regulation of bone formation by osteoclasts involves Wnt/BMP signaling and the chemokine sphingosine-1-phosphate
Categories:
Primary Cells and Media 
Authors:
Pederson L, Ruan M, Westendorf JJ, Khosla S, Oursler MJ. 
In:
Proc Natl Acad Sci USA (2008) 105(52): 20764-9 
The Role of Phospholipase D (PLD) and Grb2 in Chemotaxis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Sumoylation of peroxisome proliferator-activated receptor gamma by apoptotic cells prevents lipopolysaccharide-induced NCoR removal from kappaB binding sites mediating transrepression of proinflammatory cytokines
Categories:
Transfection 
Authors:
Jennewein C, Kuhn AM, Schmidt MV, Meilladec-Jullig V, von Knethen A, Gonzalez FJ, Bruene B 
In:
J Immunol (2008) 181(8): 5646-52 
IFN-gamma-Induced TNF-alpha expression is regulated by interferon regulatory factors 1 and 8 in mouse macrophages
Categories:
Transfection 
Authors:
Vila-del Sol V, Punzon C, Fresno M 
In:
J Immunol (2008) 181(7): 4461-70 
Promoter analysis reveals critical roles for SMAD-3 and ATF-2 in expression of IL-23 p19 in macrophages
Categories:
Transfection 
Authors:
Al-Salleeh F, Petro TM 
In:
J Immunol (2008) 181(7): 4523-33 
MyD88-independent activation of a novel actin-Cdc42/Rac pathway is required for Toll-like receptor-stimulated phagocytosis
Categories:
Transfection 
Authors:
Kong L, Ge BX 
In:
Cell Res (2008) 18(7): 745-55 
Tumor-induced suppressor of cytokine signaling 3 inhibits toll-like receptor 3 signaling in dendritic cells via binding to tyrosine kinase 2
Categories:
Transfection 
Authors:
Zeng B, Li H, Liu Y, Zhang Z, Zhang Y, Yang R 
In:
Cancer Res (2008) 68(13): 5397-404 
The macrophage-inducible C-type lectin, mincle, is an essential component of the innate immune response to Candida albicans
Categories:
Transfection 
Authors:
Wells CA, Salvage-Jones JA, Li X, Hitchens K, Butcher S, Murray RZ, Beckhouse AG, Lo YL, Manzanero S, Cobbold C, Schroder K, Ma B, Orr S, Stewart L, Lebus D, Sobieszczuk P, Hume DA, Stow J, Blanchard H, Ashman RB 
In:
J Immunol (2008) 180(11): 7404-13 
Ameobal pathogen mimivirus infects macrophages through phagocytosis
Categories:
Transfection 
Authors:
Ghigo E, Kartenbeck J, Lien P, Pelkmans L, Capo C, Mege JL, Raoult D 
In:
PLoS Pathog (2008) 4(6): e1000087 
Phosphatase SHP-1 promotes TLR- and RIG-I-activated production of type I interferon by inhibiting the kinase IRAK1
Categories:
Transfection 
Authors:
An H, Hou J, Zhou J, Zhao W, Xu H, Zheng Y, Yu Y, Liu S, Cao X 
In:
Nat Immunol (2008) 9(5): 542-50 
Phosphorylation of LXR alpha Selectively Regulates Target Gene Expression in Macrophages
Categories:
Transfection 
Authors:
Torra IP, Ismaili N, Feig JE, Xu CF, Cavasotto C, Pancratov R, Rogatsky I, Neubert TA, Fisher EA, Garabedian MJ 
In:
Mol Cell Biol (2008) 28(8): 2626-36 
Prostaglandin E Receptor type 4-Associated Protein (EPRAP) interacts directly with NF-kappa B1 and attenuates macrophage activation
Categories:
Transfection 
Authors:
Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P 
In:
J Biol Chem (2008) 283(15): 9692-703 
A novel CCCH-Zinc finger protein family regulates proinflammatory activation of macrophages
Categories:
Transfection 
Authors:
Liang J, Wang J, Azfer A, Song W, Tromp G, Kolattukudy PE, Fu M 
In:
J Biol Chem (2008) 283(10): 6337-46 
Kindlin-3 is essential for integrin activation and platelet aggregation
Categories:
Transfection 
Authors:
Moser M, Nieswandt B, Ussar S, Pozgajova M, Faessler R 
In:
Nat Med (2008) 14(3): 325-30 
Downregulation of Monocyte Chemoattractant Protein-1 Involving Short Interfering RNA Attenuates Hapten-induced Contact Hypersensitivity
Categories:
Transfection 
Authors:
Ishimoto T, Takei Y, Yuzawa Y, Hanai K, Nagahara S, Tarumi Y, Matsuo S, Kadomatsu K 
In:
Mol Ther (2008) 16(2): 387-95 
Apoptotic cell-derived sphingosine-1-phosphate promotes HuR-dependent cyclooxygenase-2 mRNA stabilization and protein expression
Categories:
Transfection 
Authors:
Johann AM, Weigert A, Eberhardt W, Kuhn AM, Barra V, von Knethen A, Pfeilschifter JM, Brüne B 
In:
J Immunol (2008) 180(2): 1239-48 
Toll-like receptor 9-stimulated monocyte chemoattractant protein-1 is mediated via JNK-cytosolic phospholipase A2-ROS signaling
Categories:
Transfection 
Authors:
Lee JG, Lee SH, Park DW, Lee SH, Yoon HS, Chin BR, Kim JH, Kim JR, Baek SH 
In:
Cell Signal (2008) 20(1): 105-11 
Mutation of Y179 on phospholipase D2 (PLD2) upregulates DNA synthesis in a PI3K-and Akt-dependent manner
Categories:
Transfection 
Authors:
Di Fulvio M, Frondorf K, Gomez-Cambronero J 
In:
Cell Signal (2008) 20(1): 176-85 
PECAM-1 Ligation Negatively Regulates TLR4 Signaling in Macrophages
Categories:
Transfection 
Authors:
Rui Y, Liu X, Li N, Jiang Y, Chen G, Cao X, Wang J 
In:
J Immunol (2007) 179(11): 7344-51 
Mechanism of Inducible Nitric Oxide Synthase Exclusion from Mycobacterial Phagosomes
Categories:
Transfection 
Authors:
Davis AS, Vergne I, Master SS, Kyei GB, Chua J, Deretic V 
In:
PLoS Pathog (2007) 3(12): e186 
Regulation of P2X4 receptors by lysosomal targeting, glycan protection and exocytosis
Categories:
Transfection 
Authors:
Qureshi OS, Paramasivam A, Yu JC, Murrell-Lagnado RD 
In:
J Cell Sci (2007) 120(Pt 21): 3838-49 
Apoptotic cells induce arginase II in macrophages, thereby attenuating NO production
Categories:
Transfection 
Authors:
Johann AM, Barra V, Kuhn AM, Weigert A, von Knethen A, Brune B 
In:
FASEB J (2007) 21(11): 2704-12 
Mitochondrial proteins Bnip3 and Bnip3L are involved in anthrax lethal toxin-induced macrophage cell death
Categories:
Transfection 
Authors:
Ha SD, Ng D, Lamothe J, Valvano MA, Han J, Kim SO 
In:
J Biol Chem (2007) 282(36): 26275-83 
Quantitative analysis of membrane remodeling at the phagocytic cup
Categories:
Transfection 
Authors:
Lee WL, Mason D, Schreiber AD, Grinstein S 
In:
Mol Biol Cell (2007) 18(8): 2883-92 
The localization and activity of sphingosine kinase 1 are coordinately-regulated with actin cytoskeletal dynamics in macrophages
Categories:
Transfection 
Authors:
Kusner DJ, Thompson CR, Melrose NA, Pitson SM, Obeid LM, Iyer SS 
In:
J Biol Chem (2007) 282(32): 23147-62 
Regulation of Toll-like receptor-mediated inflammatory response by complement in vivo
Categories:
Transfection 
Authors:
Zhang X, Kimura Y, Fang C, Zhou L, Sfyroera G, Lambris JD, Wetsel RA, Miwa T, Song WC 
In:
Blood (2007) 110(1): 228-36 
Introns regulate the rate of unstable mRNA decay
Categories:
Transfection 
Authors:
Zhao C, Hamilton T 
In:
J Biol Chem (2007) 282(28): 20230-7 
Delta-like 4 induces notch signaling in macrophages: implications for inflammation
Categories:
Transfection 
Authors:
Fung E, Tang SM, Canner JP, Morishige K, Arboleda-Velasquez JF, Cardoso AA, Carlesso N, Aster JC, Aikawa M 
In:
Circulation (2007) 115(23): 2948-56 
Role of CrkII in FCgamma receptor-mediated phagocytosis
Categories:
Transfection 
Authors:
Lee WL, Cosio G, Ireton K, Grinstein S 
In:
J Biol Chem (2007) 282(15): 11135-11143 
An Essential Role for Talin during M2-mediated Phagocytosis
Categories:
Transfection 
Authors:
Lim J, Wiedemann A, Tzircotis G, Monkley SJ, Critchley DR, Caron E 
In:
Mol Biol Cell (2007) 18(3): 976-85 
Full-length p40(phox) structure suggests a basis for regulation mechanism of its membrane binding
Categories:
Transfection 
Authors:
Honbou K, Minakami R, Yuzawa S, Takeya R, Suzuki NN, Kamakura S, Sumimoto H, Inagaki F 
In:
EMBO J (2007) 26(4): 1176-86 
Leishmania promastigotes activate PI3K/Akt signalling to confer host cell resistance to apoptosis
Categories:
Transfection 
Authors:
Ruhland A, Leal N, Kima PE 
In:
Cell Microbiol (2007) 9(1): 84-96 
Role of the Toll-like Receptor 4/NF-B Pathway in Saturated Fatty Acid-induced Inflammatory Changes in the Interaction Between Adipocytes and Macrophages
Categories:
Transfection 
Authors:
Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, Kamei Y, Ogawa Y 
In:
Arterioscler Thromb Vasc Biol (2007) 27(1): 84-91 
LAMP proteins are required for fusion of lysosomes with phagosomes
Categories:
Transfection 
Authors:
Huynh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S 
In:
EMBO J (2007) 26(2): 313-24 
Arrestin-2 and G protein-coupled receptor kinase 5 interact with NFkappa B1 p105 and negatively regulate lipopolysaccharide-stimulated ERK1/2 activation in macrophages
Categories:
Transfection 
Authors:
Parameswaran N, Pao CS, Leonhard KS, Kang DS, Kratz M, Ley SC, Benovic JL 
In:
J Biol Chem (2006) 281(45): 34159-70 
Akt/Protein kinase B modulates macrophage inflammatory response to francisella infection and confers a survival advantage in mice
Categories:
Transfection 
Authors:
Rajaram MV, Ganesan LP, Parsa KV, Butchar JP, Gunn JS, Tridandapani S 
In:
J Immunol (2006) 177(9): 6317-24 
Rab14 is critical for maintenance of Mycobacterium tuberculosis phagosome maturation arrest
Categories:
Transfection 
Authors:
Kyei GB, Vergne I, Chua J, Roberts E, Harris J, Junutula JR, Deretic V 
In:
EMBO J (2006) 25(22): 5250-5259 
MITF Interactions with 14-3-3 Modulate Differentiation of Committed Myeloid Precursors
Categories:
Transfection 
Authors:
Bronisz A, Sharma SM, Hu R, Godlewski J, Tzivion G, Mansky KC, Ostrowski MC 
In:
Mol Biol Cell (2006) 17(9): 3897-3906 
CD40 induces macrophage anti-Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes
Categories:
Transfection 
Authors:
Andrade RM, Wessendarp M, Gubbels MJ, Striepen B, Subauste CS 
In:
J Clin Invest (2006) 116(9): 2366-77 
Higher order Rab programming in phagolysosome biogenesis
Categories:
Transfection 
Authors:
Roberts EA, Chua J, Kyei GB, Deretic V 
In:
J Cell Biol (2006) 174(7): 923-9 
Signaling-dependent immobilization of acylated proteins in the inner monolayer of the plasma membrane
Categories:
Transfection 
Authors:
Corbett-Nelson EF, Mason D, Marshall JG, Collette Y, Grinstein S 
In:
J Cell Biol (2006) 174(2): 255-65 
Identification of the cAMP-Responsive Enhancer of the Murine ABCA1 Gene. Requirement for CREB1 and STAT3/4 Elements
Categories:
Transfection 
Authors:
Le Goff W, Zheng P, Brubaker G and Smith JD 
In:
Arterioscler Thromb Vasc Biol (2006) 26(3): 527-33 
Posttranslational Regulation of Tristetraprolin Subcellular Localization and Protein Stability by p38 Mitogen-Activated Protein Kinase and Extracellular Signal-Regulated Kinase Pathways
Categories:
Transfection 
Authors:
Brook M, Tchen CR, Santalucia T, McIlrath J, Arthur JS, Saklatvala J, Clark AR 
In:
Mol Cell Biol (2006) 26(6): 2408-18 
Identification of Spliced Gammaherpesvirus 68 LANA and v-Cyclin Transcripts and Analysis of Their Expression In Vivo during Latent Infection
Categories:
Transfection 
Authors:
Allen RD 3rd, Dickerson S and Speck SH 
In:
J Virol (2006) 80(4): 2055-2062 
The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1
Categories:
Transfection 
Authors:
Ai J, Maturu A, Johnson W, Wang Y, Marsh CB and Tridandapani S 
In:
Blood (2006) 107(2): 813-820 
The GRIP1:IRF3 interaction as a target for glucocorticoid receptor-mediated immunosuppression
Categories:
Transfection 
Authors:
Reily MM, Pantoja C, Hu X, Chinenov Y and Rogatsky I 
In:
EMBO J (2006) 25(1): 108-117 
Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in LPS-stimulated macrophages
Categories:
Transfection 
Authors:
Qi HY and Shelhamer JH 
In:
J Biol Chem (2005) 280(47): 38969-38975 
Interferon regulatory factor 4 negatively regulates the production of proinflammatory cytokines by macrophages in response to LPS
Categories:
Transfection 
Authors:
Honma K, Udono H, Kohno T, Yamamoto K, Ogawa A, Takemori T, Kumatori A, Suzuki S, Matsuyama T and Yui K 
In:
Proc Natl Acad Sci USA (2005) 102(44): 16001-16006 
Negative regulation of Toll-like-receptor signaling by IRF-4
Categories:
Transfection 
Authors:
Negishi H, Ohba Y, Yanai H, Takaoka A, Honma K, Yui K, Matsuyama T, Taniguchi T and Honda K 
In:
Proc Natl Acad Sci USA (2005) 102(44): 15989-15994 
Macrophage internalization of fungal beta-glucans is not necessary for initiation of related inflammatory responses
Categories:
Transfection 
Authors:
McCann F, Carmona E, Puri V, Pagano RE and Limper AH 
In:
Infect Immun (2005) 73(10): 6340-6349 
Coronin-1 Function Is Required for Phagosome Formation
Categories:
Transfection 
Authors:
Yan M, Collins RF, Grinstein S and Trimble WS 
In:
Mol Cell Biol (2005) 16(7): 3077-3087 
ERK Activation Following Macrophage FcR Ligation Leads to Chromatin Modifications at the IL-10 Locus
Categories:
Transfection 
Authors:
Lucas M, Zhang X, Prasanna V and Mosser DM 
In:
J Immunol (2005) 175(1): 469-477 
Gangliosides inhibit flagellin signaling in the absence of an effect on flagellin binding to toll-like receptor 5
Categories:
Transfection 
Authors:
West AP, Dancho BA and Mizel SB 
In:
J Biol Chem (2005) 280(10): 9482-9488 
The Nuclear IB Protein IBNS Selectively Inhibits Lipopolysaccharide-Induced IL-6 Production in Macrophages of the Colonic Lamina Propria
Categories:
Transfection 
Authors:
Hirotani T, Lee PY, Kuwata H, Yamamoto M, Matsumoto M, Kawase I, Akira S and Takeda K 
In:
J Immunol (2005) 174(6): 3650-3657 
The serine/threonine kinase Akt promotes Fc gamma receptor-mediated phagocytosis in murine macrophages through the activation of p70S6 kinase
Categories:
Transfection 
Authors:
Ganesan LP, Wei G, Pengal RA, Moldovan L, Moldovan N, Ostrowski MC and Tridandapani S 
In:
J Biol Chem (2004) 279(52): 54416-54425 
Autophagy Is a Defense Mechanism Inhibiting BCG and Mycobacterium tuberculosis Survival in Infected Macrophages
Categories:
Transfection 
Authors:
Gutierrez MG, Master SS, Singh SB, Taylor GA, Colombo MI and Deretic V 
In:
Cell (2004) 119(6): 753-766 
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