RAW 264.7

Macrophage cell line established from a tumor induced by Abelson murine leukemia virus in male adult BALB/c mouse.

Cell Type:
Macrophage
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V D-032 2e6 62-68% 67-81% Plasmid (general) 2 µg 100 µl I/II/2b
SF 96-DS-136 4e5 51-69% 82-90% Plasmid (general) 0.4 µg 20 µl Shuttle
SF

DS-136

4e5 51-69% 82-90% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (60)

Categories:
Transfection 
Authors:
Tallam A, Perumal TM, Antony PM, Jäger C, Fritz JV, Vallar L, Balling R, Del Sol A, Michelucci A. 
In:
PLoS ONE (2016) 11(2): 1-28 
Categories:
Primary Cells and Media 
Authors:
Wang Y, Dong G, Jeon HH, Elazizi M, La LB, Hameedaldeen A, Xiao E, Tian C, Alsadun S, Choi Y, Graves DT. 
In:
J Immunol (2015) 194(6): 2878-87 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lu SY, Li M, Lin YL. 
In:
Mol Biol Cell (2010) 21(10): 1763-71 
Categories:
Bioassay  
Authors:
Sugatani T, Hruska KA. 
In:
J Biol Chem (2009) 284(7): 4667-78 
Categories:
Primary Cells and Media 
Authors:
Pederson L, Ruan M, Westendorf JJ, Khosla S, Oursler MJ. 
In:
Proc Natl Acad Sci USA (2008) 105(52): 20764-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Categories:
Transfection 
Authors:
Jennewein C, Kuhn AM, Schmidt MV, Meilladec-Jullig V, von Knethen A, Gonzalez FJ, Bruene B 
In:
J Immunol (2008) 181(8): 5646-52 
Categories:
Transfection 
Authors:
Vila-del Sol V, Punzon C, Fresno M 
In:
J Immunol (2008) 181(7): 4461-70 
Categories:
Transfection 
Authors:
Al-Salleeh F, Petro TM 
In:
J Immunol (2008) 181(7): 4523-33 
Categories:
Transfection 
Authors:
Kong L, Ge BX 
In:
Cell Res (2008) 18(7): 745-55 
Categories:
Transfection 
Authors:
Zeng B, Li H, Liu Y, Zhang Z, Zhang Y, Yang R 
In:
Cancer Res (2008) 68(13): 5397-404 
Categories:
Transfection 
Authors:
Wells CA, Salvage-Jones JA, Li X, Hitchens K, Butcher S, Murray RZ, Beckhouse AG, Lo YL, Manzanero S, Cobbold C, Schroder K, Ma B, Orr S, Stewart L, Lebus D, Sobieszczuk P, Hume DA, Stow J, Blanchard H, Ashman RB 
In:
J Immunol (2008) 180(11): 7404-13 
Categories:
Transfection 
Authors:
Ghigo E, Kartenbeck J, Lien P, Pelkmans L, Capo C, Mege JL, Raoult D 
In:
PLoS Pathog (2008) 4(6): e1000087 
Categories:
Transfection 
Authors:
An H, Hou J, Zhou J, Zhao W, Xu H, Zheng Y, Yu Y, Liu S, Cao X 
In:
Nat Immunol (2008) 9(5): 542-50 
Categories:
Transfection 
Authors:
Torra IP, Ismaili N, Feig JE, Xu CF, Cavasotto C, Pancratov R, Rogatsky I, Neubert TA, Fisher EA, Garabedian MJ 
In:
Mol Cell Biol (2008) 28(8): 2626-36 
Categories:
Transfection 
Authors:
Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P 
In:
J Biol Chem (2008) 283(15): 9692-703 
Categories:
Transfection 
Authors:
Liang J, Wang J, Azfer A, Song W, Tromp G, Kolattukudy PE, Fu M 
In:
J Biol Chem (2008) 283(10): 6337-46 
Categories:
Transfection 
Authors:
Moser M, Nieswandt B, Ussar S, Pozgajova M, Faessler R 
In:
Nat Med (2008) 14(3): 325-30 
Categories:
Transfection 
Authors:
Ishimoto T, Takei Y, Yuzawa Y, Hanai K, Nagahara S, Tarumi Y, Matsuo S, Kadomatsu K 
In:
Mol Ther (2008) 16(2): 387-95 
Categories:
Transfection 
Authors:
Johann AM, Weigert A, Eberhardt W, Kuhn AM, Barra V, von Knethen A, Pfeilschifter JM, Brüne B 
In:
J Immunol (2008) 180(2): 1239-48 
Categories:
Transfection 
Authors:
Lee JG, Lee SH, Park DW, Lee SH, Yoon HS, Chin BR, Kim JH, Kim JR, Baek SH 
In:
Cell Signal (2008) 20(1): 105-11 
Categories:
Transfection 
Authors:
Di Fulvio M, Frondorf K, Gomez-Cambronero J 
In:
Cell Signal (2008) 20(1): 176-85 
Categories:
Transfection 
Authors:
Rui Y, Liu X, Li N, Jiang Y, Chen G, Cao X, Wang J 
In:
J Immunol (2007) 179(11): 7344-51 
Categories:
Transfection 
Authors:
Davis AS, Vergne I, Master SS, Kyei GB, Chua J, Deretic V 
In:
PLoS Pathog (2007) 3(12): e186 
Categories:
Transfection 
Authors:
Qureshi OS, Paramasivam A, Yu JC, Murrell-Lagnado RD 
In:
J Cell Sci (2007) 120(Pt 21): 3838-49 
Categories:
Transfection 
Authors:
Johann AM, Barra V, Kuhn AM, Weigert A, von Knethen A, Brune B 
In:
FASEB J (2007) 21(11): 2704-12 
Categories:
Transfection 
Authors:
Ha SD, Ng D, Lamothe J, Valvano MA, Han J, Kim SO 
In:
J Biol Chem (2007) 282(36): 26275-83 
Categories:
Transfection 
Authors:
Lee WL, Mason D, Schreiber AD, Grinstein S 
In:
Mol Biol Cell (2007) 18(8): 2883-92 
Categories:
Transfection 
Authors:
Kusner DJ, Thompson CR, Melrose NA, Pitson SM, Obeid LM, Iyer SS 
In:
J Biol Chem (2007) 282(32): 23147-62 
Categories:
Transfection 
Authors:
Zhang X, Kimura Y, Fang C, Zhou L, Sfyroera G, Lambris JD, Wetsel RA, Miwa T, Song WC 
In:
Blood (2007) 110(1): 228-36 
Categories:
Transfection 
Authors:
Zhao C, Hamilton T 
In:
J Biol Chem (2007) 282(28): 20230-7 
Categories:
Transfection 
Authors:
Fung E, Tang SM, Canner JP, Morishige K, Arboleda-Velasquez JF, Cardoso AA, Carlesso N, Aster JC, Aikawa M 
In:
Circulation (2007) 115(23): 2948-56 
Categories:
Transfection 
Authors:
Lee WL, Cosio G, Ireton K, Grinstein S 
In:
J Biol Chem (2007) 282(15): 11135-11143 
Categories:
Transfection 
Authors:
Lim J, Wiedemann A, Tzircotis G, Monkley SJ, Critchley DR, Caron E 
In:
Mol Biol Cell (2007) 18(3): 976-85 
Categories:
Transfection 
Authors:
Honbou K, Minakami R, Yuzawa S, Takeya R, Suzuki NN, Kamakura S, Sumimoto H, Inagaki F 
In:
EMBO J (2007) 26(4): 1176-86 
Categories:
Transfection 
Authors:
Ruhland A, Leal N, Kima PE 
In:
Cell Microbiol (2007) 9(1): 84-96 
Categories:
Transfection 
Authors:
Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, Kamei Y, Ogawa Y 
In:
Arterioscler Thromb Vasc Biol (2007) 27(1): 84-91 
Categories:
Transfection 
Authors:
Huynh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S 
In:
EMBO J (2007) 26(2): 313-24 
Categories:
Transfection 
Authors:
Parameswaran N, Pao CS, Leonhard KS, Kang DS, Kratz M, Ley SC, Benovic JL 
In:
J Biol Chem (2006) 281(45): 34159-70 
Categories:
Transfection 
Authors:
Rajaram MV, Ganesan LP, Parsa KV, Butchar JP, Gunn JS, Tridandapani S 
In:
J Immunol (2006) 177(9): 6317-24 
Categories:
Transfection 
Authors:
Kyei GB, Vergne I, Chua J, Roberts E, Harris J, Junutula JR, Deretic V 
In:
EMBO J (2006) 25(22): 5250-5259 
Categories:
Transfection 
Authors:
Bronisz A, Sharma SM, Hu R, Godlewski J, Tzivion G, Mansky KC, Ostrowski MC 
In:
Mol Biol Cell (2006) 17(9): 3897-3906 
Categories:
Transfection 
Authors:
Andrade RM, Wessendarp M, Gubbels MJ, Striepen B, Subauste CS 
In:
J Clin Invest (2006) 116(9): 2366-77 
Categories:
Transfection 
Authors:
Roberts EA, Chua J, Kyei GB, Deretic V 
In:
J Cell Biol (2006) 174(7): 923-9 
Categories:
Transfection 
Authors:
Corbett-Nelson EF, Mason D, Marshall JG, Collette Y, Grinstein S 
In:
J Cell Biol (2006) 174(2): 255-65 
Categories:
Transfection 
Authors:
Le Goff W, Zheng P, Brubaker G and Smith JD 
In:
Arterioscler Thromb Vasc Biol (2006) 26(3): 527-33 
Categories:
Transfection 
Authors:
Brook M, Tchen CR, Santalucia T, McIlrath J, Arthur JS, Saklatvala J, Clark AR 
In:
Mol Cell Biol (2006) 26(6): 2408-18 
Categories:
Transfection 
Authors:
Allen RD 3rd, Dickerson S and Speck SH 
In:
J Virol (2006) 80(4): 2055-2062 
Categories:
Transfection 
Authors:
Ai J, Maturu A, Johnson W, Wang Y, Marsh CB and Tridandapani S 
In:
Blood (2006) 107(2): 813-820 
Categories:
Transfection 
Authors:
Reily MM, Pantoja C, Hu X, Chinenov Y and Rogatsky I 
In:
EMBO J (2006) 25(1): 108-117 
Categories:
Transfection 
Authors:
Qi HY and Shelhamer JH 
In:
J Biol Chem (2005) 280(47): 38969-38975 
Categories:
Transfection 
Authors:
Honma K, Udono H, Kohno T, Yamamoto K, Ogawa A, Takemori T, Kumatori A, Suzuki S, Matsuyama T and Yui K 
In:
Proc Natl Acad Sci USA (2005) 102(44): 16001-16006 
Categories:
Transfection 
Authors:
Negishi H, Ohba Y, Yanai H, Takaoka A, Honma K, Yui K, Matsuyama T, Taniguchi T and Honda K 
In:
Proc Natl Acad Sci USA (2005) 102(44): 15989-15994 
Categories:
Transfection 
Authors:
McCann F, Carmona E, Puri V, Pagano RE and Limper AH 
In:
Infect Immun (2005) 73(10): 6340-6349 
Categories:
Transfection 
Authors:
Yan M, Collins RF, Grinstein S and Trimble WS 
In:
Mol Cell Biol (2005) 16(7): 3077-3087 
Categories:
Transfection 
Authors:
Lucas M, Zhang X, Prasanna V and Mosser DM 
In:
J Immunol (2005) 175(1): 469-477 
Categories:
Transfection 
Authors:
West AP, Dancho BA and Mizel SB 
In:
J Biol Chem (2005) 280(10): 9482-9488 
Categories:
Transfection 
Authors:
Hirotani T, Lee PY, Kuwata H, Yamamoto M, Matsumoto M, Kawase I, Akira S and Takeda K 
In:
J Immunol (2005) 174(6): 3650-3657 
Categories:
Transfection 
Authors:
Ganesan LP, Wei G, Pengal RA, Moldovan L, Moldovan N, Ostrowski MC and Tridandapani S 
In:
J Biol Chem (2004) 279(52): 54416-54425 
Categories:
Transfection 
Authors:
Gutierrez MG, Master SS, Singh SB, Taylor GA, Colombo MI and Deretic V 
In:
Cell (2004) 119(6): 753-766 
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