Neuron, hippo/cortical, rat

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nerve cells isolated from hippocampus and/or cortex of rat brain.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, rat O-003 5e6 44-64% Plasmid (general) 3 µg 100 µl I/II/2b
Neuron, rat O-003 5e6 48-70% Plasmid (general) 3 µg 100 µl I/II/2b
SCN Nucleofector Solution SCN Basic Neuro Program 1 2e4 58-70% 47-57% Plasmid (general) 0.4 µg 100 µl I/II/2b
P3

CU-133

 3e5 39-43% 57-65% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3

CU-133

 4e6 38-48% 70% Plasmid (general) 2 µg 100 µl 4D X-Unit
AD1 ER-137 2e5 50-60% 50-80% Plasmid (general) 17.5 µg 350 µl 4D Y-Unit
P3

96-CU-133

 3e5 30-50%


Plasmid (general) 400 ng 20 µl 96-well

Citations (148)

Comparison of Different Spike Train Synchrony Measures Regarding Their Robustness to Erroneous Data From Bicuculline-Induced Epileptiform Activity
Categories:
Primary Cells and Media 
Authors:
Manuel Ciba , Robert Bestel , Christoph Nick , Guilherme Ferraz de Arruda , Thomas Peron , Comin César Henrique , Luciano da Fontoura Costa , Francisco Aparecido Rodrigues , Christiane Thielemann  
In:
Neural Comput. (2020) 32(5): 887-911 
A pyruvate dehydrogenase kinase inhibitor prevents retinal cell death and improves energy metabolism in rat retinas after ischemia/reperfusion injury 
Categories:
Primary Cells and Media 
Authors:
Sato K, Mochida S, Tomimoto D, Konuma T, Kiyota N, Tsuda S, Shiga Y, Omodaka K, Nakazawa T 
In:
Exp Eye Res (2020) 193: 107997 
Translating intracarotid artery transplantation of bone marrow-derived NCS-01 cells for ischemic stroke: Behavioral and histological readouts and mechanistic insights into stem cell therapy.
Categories:
Primary Cells and Media 
Authors:
Kaneko Y, Lee JY, Tajiri N, Tuazon JP, Lippert T, Russo E, Yu SJ, Bonsack B, Corey S, Coats AB, Kingsbury C, Chase TN, Koga M, Borlongan CV 
In:
STEM CELLS TRANSLATIONALMEDICINE (2019) 10: 1002 
Long-term electromagnetic exposure of developing neuronal networks: A flexible experimental setup
Categories:
Primary Cells and Media 
Authors:
Oster S, Daus AW, Erbes C, Goldhammer M, Bochtler U, Thielemann C 
In:
Other (2016) 37(4): 264-278 
Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications
Categories:
Primary Cells and Media 
Authors:
Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney KM, Lu Y,Hall D, Ross JD 
In:
Other (2016) 9690: ePub 
Growth and structural discrimination of cortical neurons on randomly oriented and vertically aligned dense carbon nanotube networks.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nick C, Yadav S, Joshi R, Thielemann C, Schneider JJ. 
In:
Other (2014) 5: 1575-9 
Neurotoxicity and mode of action of N, N-diethyl-meta-toluamide (DEET).
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Swale DR, Sun B, Tong F, Bloomquist JR. 
In:
PLoS ONE (2014) 9(8): ePub 
Are Carbon Nanotube Microelectrodes Manufactured from Dispersion Stable Enough for Neural Interfaces
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nick C, Thielemann C 
In:
Other (2014) ePub: ePub 
BMP2 shows neurotrophic effects including neuroprotection against neurodegeneration.
Categories:
Primary Cells and Media 
Authors:
Saglam A, Kim S, Ahn K, Oh I, Lee KH 
In:
Other (2014) 25(8): 549-555 
DrCell – A Software Tool for the Analysis of Cell Signals Recorded with Extracellular Microelectrodes
Categories:
Primary Cells and Media 
Authors:
Nick C, Goldhammer M, Bestel R, Steger F, Daus AW, Thielemann C 
In:
Other (2014) 7(2): 96-109 
Study of neuron survival on polypyrrole-embedded single-walled carbon nanotube substrates for long term growth conditions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hernández-Ferrer J1, Pérez-Bruzón RN, Azanza MJ, González M, Del Moral R, Ansón-Casaos A, de la Fuente JM, Marijuan PC, Martínez MT. 
In:
J Biomed Mater Res A (2014) Epub: 1-12 
Hsp90 chaperone inhibitor 17-AAG attenuates Aß-induced synaptic toxicity and memory impairment.
Categories:
Transfection 
Authors:
Chen Y1, Wang B, Liu D, Li JJ, Xue Y, Sakata K, Zhu LQ, Heldt SA, Xu H, Liao FF 
In:
J Neurosci (2014) 34 (7): 2464-2470 
Hippocampal Neuron Protecting Effect of Propofol Against Hypoxia/Reoxygenation via Inducing Nerve Growth Factor
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Feng YN, Shi Q, Ma H, Fang J 
In:
Other (2014) 4(123): ePub 
In vitro and in vivo optimization of infrared laser treatment for injured peripheral nerves
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Anders JJ, Moges H, Wu X, Erbele ID, Alberico SL, Saidu EK, Smith JT, Pryor BA. 
In:
Other (2014) 46(1): 34-45 
The human language and epilepsy associated gene SRPX2 regulates synapse formation and vocalization in mice
Categories:
Transfection 
Authors:
Sia GM, Clem RL, Huganir RL. 
In:
Science (2013) 342: 987-991 
Heating during infrared neural stimulation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liljemalm R, Nyberg T, von Holst H. 
In:
Lasers in Surgery and Medicine (2013) 45(7): 469-81 
Polymer peel-off mask for high-resolution surface derivatization, neuron placement and guidance
Categories:
Primary Cells and Media 
Authors:
Martinez D, Py C, Denhoff M, Monette R, Comas T, Krantis A, Mealing G 
In:
Biotechnol Bioeng (2013) 110(8): 2236-41 
Interactions between aCaMKII and calmodulin in living cells: conformational changes arising from CaM-dependent and -independent relationships
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kato K, Iwamoto T, Kida S. 
In:
Molecular Brain (2013) 6(37): 37 
Multielectrode array analysis of cerebrospinal fluid in Alzheimer\'s disease versus mild cognitive impairment: a potential diagnostic and treatment biomarker
Categories:
Primary Cells and Media 
Authors:
Görtz P, Siebler M, Ihl R, Henning U, Luckhaus C, Supprian T, Lange-Asschenfeldt C 
In:
Biochem Biophys Res Commun (2013) 434(2): 293-7 
Mechanistic role for a novel glucocorticoid-KLF11 (TIEG2) protein pathway in stress-induced monoamine oxidase A expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Grunewald M, Johnson S, Lu D, Wang Z, Lomberk G, Albert PR, Stockmeier CA, Meyer JH, Urrutia R, Miczek KA, Austin MC, Wang J, Paul IA, Woolverton WL, Seo S, Sittman DB, Ou XM 
In:
J Biochem (2012) 287(29): 24195-206 
From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology
Categories:
Primary Cells and Media 
Authors:
Py C, Martina M, Diaz-Quijada GA, Luk CC, Martinez D, Denhoff MW, Charrier A, Comas T, Monette R, Krantis A, Syed NI, Mealing GA 
In:
Frontiers in Pharmacology (2011) 2: 51 
Degradation of Proteins for Neural Cell Adhesion Patterning by Carbon Negative-Ion Implantation
Categories:
Primary Cells and Media 
Authors:
Tsuji H, Sommani P, Sato S, Gotoh Y, Ishikawa J, Takaoka G 
In:
Other (2011) 36(3): 293-296 
Differential induction of rat neuronal excitotoxic cell death by human immunodeficiency virus type 1 clade B and C tat proteins
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Campbell GR, Watkins JD, Loret EP, Spector SA 
In:
Aids Research and Human Retroviruses (2011) 27(6): 647-54 
A hybrid approach for the control of axonal outgrowth: preliminary simulation results.
Categories:
Primary Cells and Media 
Authors:
Ciofani G, Sergi PN, Carpaneto J, Micera S 
In:
Medical & Bioengineering & Computing (2011) 49(2): 163-70 
Kinase/phosphatase overexpression reveals pathways regulating hippocampal neuron morphology
Categories:
Transfection 
Authors:
Buchser WJ, Slepak TI, Gutierrez-Arenas O, Bixby JL, Lemmon VP 
In:
Mol Syst Biol (2010) 6: 1(16) 
CRF receptor 1 regulates anxiety behavior via sensitization of 5-HT2 receptor signaling
Categories:
Primary Cells and Media 
Authors:
Magalhaes AC, Holmes KD, Dale LB, Comps-Agrar L, Lee D, Yadav PN, Drysdale L, Poulter MO, Roth BL, Pin JP, Anisman H, Ferguson SS 
In:
Nat Neurosci (2010) 13(5): 622-9 
A chemical screen identifies novel compounds that overcome glial-mediated inhibition of neuronal regeneration
Categories:
Primary Cells and Media 
Authors:
Usher LC, Johnstone A, Ertürk A, Hu Y, Strikis D, Wanner IB, Moorman S, Lee JW, Min J, Ha HH, Duan Y, Hoffman S, Goldberg JL, Bradke F, Chang YT, Lemmon VP, Bixby JL 
In:
J Neurosci (2010) 30(13): 4693-706 
Antidepressant fluoxetine suppresses neuronal growth from both vertebrate and invertebrate neurons and perturbs synapse formation between Lymnaea neurons
Categories:
Primary Cells and Media 
Authors:
Xu F, Luk C, Richard MP, Zaidi W, Farkas S, Getz A, Lee A, van Minnen J, Syed NI. 
In:
Eur J Neurosci (2010) 31(6): 994-1005 
The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection
Categories:
Primary Cells and Media 
Authors:
Haedicke J, Brown C, Naghavi MH. 
In:
Proc Natl Acad Sci USA (2009) 106(33): 14040-5 
Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching
Categories:
Transfection 
Authors:
Li H, Chen G, Zhou B, Duan S 
In:
Mol Biol Cell (2008) 19(11): 4804-4813 
Phosphorylation of Profilin by ROCK1 Regulates Polyglutamine Aggregation
Categories:
Transfection 
Authors:
Shao J, Welch WJ, Diprospero NA, Diamond MI 
In:
Mol Cell Biol (2008) 28(17): 5196-208 
Tuberous sclerosis complex proteins control axon formation
Categories:
Transfection 
Authors:
Choi YJ, Di Nardo A, Kramvis I, Meikle L, Kwiatkowski DJ, Sahin M, He X 
In:
Genes Dev (2008) 22(18): 2485-95 
The ubiquitin-like protein Plic-1 enhances the membrane insertion of GABAA receptors by increasing their stability within the endoplasmic reticulum
Categories:
Transfection 
Authors:
Saliba RS, Pangalos M, Moss SJ 
In:
J Biol Chem (2008) 283(27): 18538-44 
Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons
Categories:
Transfection 
Authors:
Zeitelhofer M, Karra D, Macchi P, Tolino M, Thomas S, Schwarz M, Kiebler M, Dahm R 
In:
J Neurosci (2008) 28(30): 7555-62 
Mitochondrial Ca2+ overload underlies Abeta oligomers neurotoxicity providing an unexpected mechanism of neuroprotection by NSAIDs
Categories:
Transfection 
Authors:
Sanz-Blasco S, Valero RA, Rodriguez-Crespo I, Villalobos C, Nunez L 
In:
PLoS ONE (2008) 3(7): e2718 
Cholesterol Loss Enhances TrkB Signaling in Hippocampal Neurons Aging in Vitro
Categories:
Transfection 
Authors:
Martin MG, Perga S, Trovܲ L, Rasola A, Holm P, Rantamäki T, Harkany T, Castrén E, Chiara F, Dotti CG 
In:
Mol Biol Cell (2008) 19(5): 2101-2112 
Redefining the role of metallothionein within the injured brain: Extracellular metallothioneins play an important role in the astrocyte-neuron response to injury
Categories:
Transfection 
Authors:
Chung RS, Penkowa M, Dittmann J, King CE, Bartlett C, Asmussen JW, Hidalgo J, Carrasco J, Leung YK, Walker AK, Fung SJ, Dunlop SA, Fitzgerald M, Beazley LD, Chuah MI, Vickers JC, West AK 
In:
J Biol Chem (2008) 283(22): 15349-58 
Clathrin-dependent endocytosis is required for TrkB-dependent Akt-mediated neuronal protection and dendritic growth
Categories:
Transfection 
Authors:
Zheng J, Shen WH, Lu TJ, Zhou Y, Chen Q, Wang Z, Xiang T, Zhu YC, Zhang C, Duan S, Xiong ZQ 
In:
J Biol Chem (2008) 283(19): 13280-8 
The Microtubule-severing Proteins Spastin and Katanin Participate Differently in the Formation of Axonal Branches
Categories:
Transfection 
Authors:
Yu W, Qiang L, Solowska JM, Karabay A, Korulu S, Baas PW 
In:
Mol Biol Cell (2008) 19(4): 1485-98 
Tonic activation of CXC chemokine receptor 4 in immature granule cells supports neurogenesis in the adult dentate gyrus
Categories:
Transfection 
Authors:
Kolodziej A, Schulz S, Guyon A, Wu DF, Pfeiffer M, Odemis V, Höllt V, Stumm R 
In:
J Neurosci (2008) 28(17): 4488-500 
Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength
Categories:
Transfection 
Authors:
Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ 
In:
J Neurosci (2008) 28(11): 2753-65 
Regulation of PI3K signalling by the phosphatidylinositol transfer protein PITP-alpha during axonal extension in hippocampal neurons
Categories:
Transfection 
Authors:
Cosker KE, Shadan S, van Diepen M, Morgan C, Li M, Allen-Baume V, Hobbs C, Doherty P, Cockcroft S, Eickholt BJ 
In:
J Cell Sci (2008) 121(Pt 6): 796-803 
The clustering of GABA(A) receptor subtypes at inhibitory synapses is facilitated via the direct binding of receptor alpha 2 subunits to gephyrin
Categories:
Transfection 
Authors:
Tretter V, Jacob TC, Mukherjee J, Fritschy JM, Pangalos MN, Moss SJ 
In:
J Neurosci (2008) 28(6): 1356-65 
Rapid tumor necrosis factor alpha-induced exocytosis of glutamate receptor 2-lacking AMPA receptors to extrasynaptic plasma membrane potentiates excitotoxicity
Categories:
Transfection 
Authors:
Leonoudakis D, Zhao P, Beattie EC 
In:
J Neurosci (2008) 28(9): 2119-30 
Quantitative and functional analyses of spastin in the nervous system: implications for hereditary spastic paraplegia
Categories:
Transfection 
Authors:
Solowska JM, Morfini G, Falnikar A, Himes BT, Brady ST, Huang D, Baas PW 
In:
J Neurosci (2008) 28(9): 2147-57 
The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM
Categories:
Transfection 
Authors:
Yap CC, Wisco D, Kujala P, Lasiecka ZM, Cannon JT, Chang MC, Hirling H, Klumperman J, Winckler B 
In:
J Cell Biol (2008) 180(4): 827-42 
Microtubule stabilization specifies initial neuronal polarization
Categories:
Transfection 
Authors:
Witte H, Neukirchen D, Bradke F 
In:
J Cell Biol (2008) 180(3): 619-32 
Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities
Categories:
Transfection 
Authors:
Ruan B, Pong K, Jow F, Bowlby M, Crozier RA, Liu D, Liang S, Chen Y, Mercado ML, Feng X, Bennett F, von Schack D, McDonald L, Zaleska MM, Wood A, Reinhart PH, Magolda RL, Skotnicki J, Pangalos MN, Koehn FE, Carter GT, Abou-Gharbia M, Graziani EI 
In:
Proc Natl Acad Sci USA (2008) 105(1): 33-8 
The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases
Categories:
Transfection 
Authors:
Richter M, Murai KK, Bourgin C, Pak DT, Pasquale EB 
In:
J Neurosci (2007) 27(51): 14205-15 
FMRP phosphorylation reveals an immediate-early signaling pathway triggered by group I mGluR and mediated by PP2A.
Categories:
Primary Cells and Media 
Authors:
Narayanan U, Nalavadi V, Nakamoto M, Pallas DC, Ceman S, Bassell GJ, Warren ST. 
In:
J Neurosci (2007) 27(52): 14349-57 
Neurabin-I is phosphorylated by cdk5: implications for neuronal morphogenesis and cortical migration
Categories:
Transfection 
Authors:
Causeret F, Jacobs T, Terao M, Heath O, Hoshino M, Nikolic M 
In:
Mol Biol Cell (2007) 18(11): 4327-42 
Evidence that the BLOC-1 protein dysbindin modulates dopamine D2 receptor internalization and signaling but not D1 internalization
Categories:
Transfection 
Authors:
Iizuka Y, Sei Y, Weinberger DR, Straub RE 
In:
J Neurosci (2007) 27(45): 12390-5 
Activity-dependent ubiquitination of GABA(A) receptors regulates their accumulation at synaptic sites
Categories:
Transfection 
Authors:
Saliba RS, Michels G, Jacob TC, Pangalos MN, Moss SJ 
In:
J Neurosci (2007) 27(48): 13341-51 
Alpha2-Chimaerin interacts with EphA4 and regulates EphA4-dependent growth cone collapse
Categories:
Transfection 
Authors:
Shi L, Fu WY, Hung KW, Porchetta C, Hall C, Fu AK, Ip NY 
In:
Proc Natl Acad Sci USA (2007) 104(41): 16347-52 
Role of kinase suppressor of Ras-1 in neuronal survival signaling by extracellular signal-regulated kinase 1/2
Categories:
Transfection 
Authors:
Szatmari E, Kalita KB, Kharebava G, Hetman M 
In:
J Neurosci (2007) 27(42): 11389-400 
Astrocytes regulate GluR2 expression in motor neurons and their vulnerability to excitotoxicity
Categories:
Transfection 
Authors:
Van Damme P, Bogaert E, Dewil M, Hersmus N, Kiraly D, Scheveneels W, Bockx I, Braeken D, Verpoorten N, Verhoeven K, Timmerman V, Herijgers P, Callewaert G, Carmeliet P, Van Den Bosch L, Robberecht W 
In:
Proc Natl Acad Sci USA (2007) 104(37): 14825-30 
The neuronal Arf GAP centaurin 1 modulates dendritic differentiation
Categories:
Transfection 
Authors:
Moore CD, Thacker EE, Larimore J, Gaston D, Underwood A, Kearns B, Patterson SI, Jackson T, Chapleau C, Pozzo-Miller L, Theibert A 
In:
J Cell Sci (2007) 120(Pt 15): 2683-93 
Localized activation of p21-activated kinase controls neuronal polarity and morphology
Categories:
Transfection 
Authors:
Jacobs T, Causeret F, Nishimura YV, Terao M, Norman A, Hoshino M, Nikolić M 
In:
J Neurosci (2007) 27(32): 8604-15 
Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth
Categories:
Transfection 
Authors:
Hisata S, Sakisaka T, Baba T, Yamada T, Aoki K, Matsuda M, Takai Y 
In:
J Cell Biol (2007) 178(5): 843-60 
Critical role of calpain I in mitochondrial release of apoptosis-inducing factor in ischemic neuronal injury
Categories:
Transfection 
Authors:
Cao G, Xing J, Xiao X, Liou AK, Gao Y, Yin XM, Clark RS, Graham SH, Chen J 
In:
J Neurosci (2007) 27(35): 9278-93 
Singar1, a novel RUN domain-containing protein, suppresses formation of surplus axons for neuronal polarity
Categories:
Transfection 
Authors:
Mori T, Wada T, Suzuki T, Kubota Y, Inagaki N 
In:
J Biol Chem (2007) 282(27): 19884-93 
Dishevelled promotes axon differentiation by regulating atypical protein kinase C
Categories:
Transfection 
Authors:
Zhang X, Zhu J, Yang GY, Wang QJ, Qian L, Chen YM, Chen F, Tao Y, Hu HS, Wang T, Luo ZG 
In:
Nat Cell Biol (2007) 9(7): 743-54 
The Nogo-66 receptor NgR1 is required only for the acute growth cone-collapsing but not the chronic growth-inhibitory actions of myelin inhibitors
Categories:
Transfection 
Authors:
Chivatakarn O, Kaneko S, He Z, Tessier-Lavigne M, Giger RJ 
In:
J Neurosci (2007) 27(27): 7117-24 
Transcription factor NF-kappaB is transported to the nucleus via cytoplasmic dynein/dynactin motor complex in hippocampal neurons
Categories:
Transfection 
Authors:
Mikenberg I, Widera D, Kaus A, Kaltschmidt B, Kaltschmidt C 
In:
PLoS ONE (2007) 2: e589 
High-efficiency transfection of mammalian neurons via nucleofection
Categories:
Transfection 
Authors:
Zeitelhofer M, Vessey JP, Xie Y, Tübing F, Thomas S, Kiebler M, Dahm R 
In:
Nat Protocols (2007) 2(7): 1692-704 
Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms
Categories:
Transfection 
Authors:
Dzhashiashvili Y, Zhang Y, Galinska J, Lam I, Grumet M, Salzer JL 
In:
J Cell Biol (2007) 177(5): 857-70 
The B''/PR72 subunit mediates Ca2+-dependent dephosphorylation of DARPP-32 by protein phosphatase 2A
Categories:
Transfection 
Authors:
Ahn JH, Sung JY, McAvoy T, Nishi A, Janssens V, Goris J, Greengard P, Nairn AC 
In:
Proc Natl Acad Sci USA (2007) 104(23): 9876-81 
Truncations of amphiphysin I by calpain inhibit vesicle endocytosis during neural hyperexcitation
Categories:
Transfection 
Authors:
Wu Y, Liang S, Oda Y, Ohmori I, Nishiki TI, Takei K, Matsui H, Tomizawa K 
In:
EMBO J (2007) 26(12): 2981-90 
Regulation of dendritic excitability by activity-dependent trafficking of the A-type K+ channel subunit Kv4.2 in hippocampal neurons
Categories:
Transfection 
Authors:
Kim J, Jung SC, Clemens AM, Petralia RS, Hoffman DA 
In:
Neuron (2007) 54(6): 933-47 
Cytoplasmic dynein and LIS1 are required for microtubule advance during growth cone remodeling and fast axonal outgrowth
Categories:
Transfection 
Authors:
Grabham PW, Seale GE, Bennecib M, Goldberg DJ, Vallee RB 
In:
J Neurosci (2007) 27(21): 5823-34 
The Novel GTPase Rit Differentially Regulates Axonal and Dendritic Growth
Categories:
Transfection 
Authors:
Lein PJ, Guo X, Shi GX, Moholt-Siebert M, Bruun D, Andres DA 
In:
J Neurosci (2007) 27(17): 4725-4736 
GAP-43 regulates NCAM-180-mediated neurite outgrowth
Categories:
Transfection 
Authors:
Korshunova I, Novitskaya V, Kiryushko D, Pedersen N, Kolkova K, Kropotova E, Mosevitsky M, Rayko M, Morrow JS, Ginzburg I, Berezin V, Bock E 
In:
J Neurochem (2007) 100(6): 1599-612 
Regulation of S6 kinase 1 activation by phosphorylation at Ser411
Categories:
Transfection 
Authors:
Hou Z, He L, Qi RZ 
In:
J Biol Chem (2007) 282(10): 6922-8 
Phospholipase C is required for changes in postsynaptic structure and function associated with NMDA receptor-dependent long-term depression
Categories:
Transfection 
Authors:
Horne EA, Dell'Acqua ML 
In:
J Neurosci (2007) 27(13): 3523-34 
Regulation and function of glycogen synthase kinase-3 isoforms in neuronal survival
Categories:
Transfection 
Authors:
Liang MH, Chuang DM 
In:
J Biol Chem (2007) 282(6): 3904-17 
Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface
Categories:
Transfection 
Authors:
Mizokami A, Kanematsu T, Ishibashi H, Yamaguchi T, Tanida I, Takenaka K, Nakayama KI, Fukami K, Takenawa T, Kominami E, Moss SJ, Yamamoto T, Nabekura J, Hirata M 
In:
J Neurosci (2007) 27(7): 1692-701 
R-Ras controls axon specification upstream of GSK-3beta through integrin-linked kinase
Categories:
Transfection 
Authors:
Oinuma I, Katoh H, Negishi M 
In:
J Biol Chem (2007) 282(1): 303-318 
DISC1 regulates neurotrophin-induced axon elongation via interaction with Grb2
Categories:
Transfection 
Authors:
Shinoda T, Taya S, Tsuboi D, Hikita T, Matsuzawa R, Kuroda S, Iwamatsu A, Kaibuchi K 
In:
J Neurosci (2007) 27(1): 4-14 
Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase
Categories:
Transfection 
Authors:
Kuramoto N, Wilkins ME, Fairfax BP, Revilla-Sanchez R, Terunuma M, Tamaki K, Iemata M, Warren N, Couve A, Calver A, Horvath Z, Freeman K, Carling D, Huang L, Gonzales C, Cooper E, Smart TG, Pangalos MN, Moss SJ 
In:
Neuron (2007) 53(2): 233-47 
Serum- and Glucocorticoid-Inducible Kinase 1 (SGK1) Increases Neurite Formation through Microtubule Depolymerization by SGK1 and by SGK1 Phosphorylation of Tau
Categories:
Transfection 
Authors:
Yang YC, Lin CH, Lee EH 
In:
Mol Cell Biol (2006) 26(22): 8357-70 
Transgenic Rescue of ataxia Mice with Neuronal-Specific Expression of Ubiquitin-Specific Protease 14
Categories:
Transfection 
Authors:
Crimmins S, Jin Y, Wheeler C, Huffman AK, Chapman C, Dobrunz LE, Levey A, Roth KA, Wilson JA, Wilson SM 
In:
J Neurosci (2006) 26(44): 11423-31 
Tau-dependent microtubule disassembly initiated by prefibrillar -amyloid
Categories:
Transfection 
Authors:
King ME, Kan HM, Baas PW, Erisir A, Glabe CG, Bloom GS 
In:
J Cell Biol (2006) 175(4): 541-6 
Molecular network and chromosomal clustering of genes involved in synaptic plasticity in the hippocampus
Categories:
Transfection 
Authors:
Park CS, Gong R, Stuart J, Tang SJ 
In:
J Biol Chem (2006) 281(40): 30195-211 
Differential roles of glycogen synthase kinase-3 isoforms in the regulation of transcriptional activation
Categories:
Transfection 
Authors:
Liang MH, Chuang DM 
In:
J Biol Chem (2006) 281(41): 30479-30484 
Antagonistic forces generated by cytoplasmic dynein and myosin-II during growth cone turning and axonal retraction
Categories:
Transfection 
Authors:
Myers KA, Tint I, Nadar CV, He Y, Black MM, Baas PW 
In:
Traffic (2006) 7(10): 1333-51 
Drug-induced activation of SREBP-controlled lipogenic gene expression in CNS-related cell lines: marked differences between various antipsychotic drugs
Categories:
Primary Cells and Media 
Authors:
Fernø J, Skrede S, Vik-Mo AO, Håvik B, Steen VM. 
In:
BMC Neurosci (2006) 7: 69 
Regulation of neuronal morphology by toca-1, an F-BAR/EFC protein that induces plasma membrane invagination
Categories:
Transfection 
Authors:
Kakimoto T, Katoh H, Negishi M 
In:
J Biol Chem (2006) 281(39): 29042-53 
SNAP-25/syntaxin 1A complex functionally modulates neurotransmitter GABA reuptake
Categories:
Transfection 
Authors:
Fan HP, Fan FJ, Bao L, Pei G 
In:
J Biol Chem (2006) 281(38): 28174-84 
Activated c-Jun N-terminal kinase is required for axon formation
Categories:
Transfection 
Authors:
Oliva AA Jr, Atkins CM, Copenagle L, Banker GA 
In:
J Neurosci (2006) 26(37): 9462-70 
The Rac Activator DOCK7 Regulates Neuronal Polarity through Local Phosphorylation of Stathmin/Op18
Categories:
Transfection 
Authors:
Watabe-Uchida M, John KA, Janas JA, Newey SE, Van Aelst L 
In:
Neuron (2006) 51(6): 727-39 
Role of megakaryoblastic acute leukemia-1 in ERK1/2-dependent stimulation of serum response factor-driven transcription by BDNF or increased synaptic activity
Categories:
Transfection 
Authors:
Kalita K, Kharebava G, Zheng JJ, Hetman M 
In:
J Neurosci (2006) 26(39): 10020-32 
Cytotoxicity and cytoprotective activity of naphthalenediols in rat cortical neurons
Categories:
Primary Cells and Media 
Authors:
Flueraru M, So R, Willmore WG, Poulter MO, Durst T, Charron M, Wright JS 
In:
Chemical Research in Toxicology (2006) 19(9): 1221-7 
Anaplastic lymphoma kinase is a dependence receptor whose proapoptotic functions are activated by caspase cleavage
Categories:
Transfection 
Authors:
Mourali J, Benard A, Lourenco FC, Monnet C, Greenland C, Moog-Lutz C, Racaud-Sultan C, Gonzalez-Dunia D, Vigny M, Mehlen P, Delsol G, Allouche M 
In:
Mol Cell Biol (2006) 26(16): 6209-22 
N-syndecan deficiency impairs neural migration in brain
Categories:
Transfection 
Authors:
Hienola A, Tumova S, Kulesskiy E, Rauvala H 
In:
J Cell Biol (2006) 174(4): 569-80 
Interneurite affinity is regulated by heterophilic nectin interactions in concert with the cadherin machinery
Categories:
Transfection 
Authors:
Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M 
In:
J Cell Biol (2006) 174(1): 141-51 
Sema4D/plexin-B1 activates GSK-3beta through R-Ras GAP activity, inducing growth cone collapse
Categories:
Transfection 
Authors:
Ito Y, Oinuma I, Katoh H, Kaibuchi K, Negishi M 
In:
EMBO Rep (2006) 7(7): 704-9 
Palmitoylation of huntingtin by HIP14is essential for its trafficking and function
Categories:
Transfection 
Authors:
Yanai A, Huang K, Kang R, Singaraja RR, Arstikaitis P, Gan L, Orban PC, Mullard A, Cowan CM, Raymond LA, Drisdel RC, Green WN, Ravikumar B, Rubinsztein DC, El-Husseini A, Hayden MR 
In:
Nat Neurosci (2006) 9(6): 824-831 
Regulation of NMDA receptor trafficking and function by striatal-enriched tyrosine phosphatase (STEP)
Categories:
Transfection 
Authors:
Braithwaite SP, Adkisson M, Leung J, Nava A, Masterson B, Urfer R, Oksenberg D, Nikolich K 
In:
Eur J Neurosci (2006) 23(11): 2847-56 
Effects of dynactin disruption and Dynein depletion on axonal microtubules
Categories:
Transfection 
Authors:
Ahmad FJ, He Y, Myers KA, Hasaka TP, Francis F, Black MM, Baas PW 
In:
Traffic (2006) 7(5): 524-37 
Microtubule affinity-regulating kinase 2 functions downstream of the PAR-3/PAR-6/atypical PKC complex in regulating hippocampal neuronal polarity
Categories:
Transfection 
Authors:
Chen YM, Wang QJ, Hu HS, Yu PC, Zhu J, Drewes G, Piwnica-Worms H, Luo ZG 
In:
Proc Natl Acad Sci USA (2006) 103(22): 8534-9 
Cell Survival through Trk Neurotrophin Receptors Is Differentially Regulated by Ubiquitination
Categories:
Transfection 
Authors:
Arevalo JC, Waite J, Rajagopal R, Beyna M, Chen ZY, Lee FS, Chao MV 
In:
Neuron (2006) 50(4): 549-59 
Eph receptors inactivate R-Ras through different mechanisms to achieve cell repulsion
Categories:
Transfection 
Authors:
Dail M, Richter M, Godement P, Pasquale EB 
In:
J Cell Sci (2006) 119(Pt 7): 1244-54 
SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development
Categories:
Transfection 
Authors:
Zhu PP, Soderblom C, Tao-Cheng JH, Stadler J, Blackstone C 
In:
Hum Mol Genet (2006) 15(8): 1343-53 
Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
Categories:
Transfection 
Authors:
Loomis PA, Kelly AE, Zheng L, Changyaleket B, Sekerkova G, Mugnaini E, Ferreira A, Mullins RD, Bartles JR 
In:
J Cell Sci (2006) 119(Pt 8): 1655-65 
Tubulin-mediated binding of human immunodeficiency virus-1 Tat to the cytoskeleton causes proteasomal-dependent degradation of microtubule-associated protein 2 and neuronal damage
Categories:
Transfection 
Authors:
Aprea S, Del Valle L, Mameli G, Sawaya BE, Khalili K, Peruzzi F 
In:
J Neurosci (2006) 26(15): 4054-62 
Ligand-Dependent Cleavage of the P75 Neurotrophin Receptor Is Necessary for NRIF Nuclear Translocation and Apoptosis in Sympathetic Neurons
Categories:
Transfection 
Authors:
Kenchappa RS, Zampieri N, Chao MV, Barker PA, Teng HK, Hempstead BL, Carter BD 
In:
Neuron (2006) 50(2): 219-32 
Cdc42 and Actin Control Polarized Expression of TI-VAMP Vesicles to Neuronal Growth Cones and Their Fusion with the Plasma Membrane
Categories:
Transfection 
Authors:
Alberts P, Rudge R, Eirinopoulou T, Danglot L, Gauthier-Rouviere C and Galli T 
In:
Mol Biol Cell (2006) 17(3): 1194-203 
A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon
Categories:
Transfection 
Authors:
Pan Z, Kao T, Horvath Z, Lemos J, Sul JY, Cranstoun SD, Bennett V, Scherer SS, Cooper EC 
In:
J Neurosci (2006) 26(10): 2599-613 
cAMP-dependent protein kinase postsynaptic localization regulated by NMDA receptor activation through translocation of an A-kinase anchoring protein scaffold protein
Categories:
Transfection 
Authors:
Smith KE, Gibson ES, Dell'Acqua ML 
In:
J Neurosci (2006) 26(9): 2391-402 
Tau protects microtubules in the axon from severing by katanin
Categories:
Transfection 
Authors:
Qiang L, Yu W, Andreadis A, Luo M, Baas PW 
In:
J Neurosci (2006) 26(12): 3120-9 
Elk-1 associates with the mitochondrial permeability transition pore complex in neurons
Categories:
Transfection 
Authors:
Barrett LE, Van Bockstaele EJ, Sul JY, Takano H, Haydon PG, Eberwine JH 
In:
Proc Natl Acad Sci USA (2006) 103(13): 5155-60 
A change in the selective translocation of the Kinesin-1 motor domain marks the initial specification of the axon
Categories:
Transfection 
Authors:
Jacobson C, Schnapp B, Banker GA 
In:
Neuron (2006) 49(6): 797-804 
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling
Categories:
Transfection 
Authors:
Volmer R, Monnet C, Gonzalez-Dunia D 
In:
PLoS Pathog (2006) 2(3): e19 
Alternative splicing of human metabotropic glutamate receptor 3
Categories:
Transfection 
Authors:
Sartorius LJ, Nagappan G, Lipska BK, Lu B, Sei Y, Ren-Patterson R, Li Z, Weinberger DR, Harrison PJ 
In:
J Neurochem (2006) 96(4): 1139-48 
A preformed complex of postsynaptic proteins is involved in excitatory synapse development
Categories:
Transfection 
Authors:
Gerrow K, Romorini S, Nabi SM, Colicos MA, Sala C, El-Husseini A 
In:
Neuron (2006) 49(4): 547-62 
Functional analysis of SIRP in the growth cone
Categories:
Transfection 
Authors:
Wang XX and Pfenninger KH 
In:
J Cell Sci (2006) 119(Pt 1): 172-183 
The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
Categories:
Transfection 
Authors:
Goetze B, Tuebing F, Xie Y, Dorostkar MM, Thomas S, Pehl U, Boehm S, Macchi P and Kiebler MA 
In:
J Cell Biol (2006) 172(2): 221-231 
Nucleofection of primary neurons
Categories:
Transfection 
Authors:
Gartner A, Collin L, Lalli G 
In:
Methods Enzymol (2006) 406: 374-88 
A Novel Endocytic Recycling Signal Distinguishes Biological Responses of Trk Neurotrophin Receptors
Categories:
Transfection 
Authors:
Chen ZY, Ieraci A, Tanowitz M and Lee FS 
In:
Mol Biol Cell (2005) 16(12): 5761-5772 
The BTB domain of the nuclear matrix protein NRP/B is required for neurite outgrowth
Categories:
Transfection 
Authors:
Kim TA, Jiang S, Seng S, Cha K, Avraham HK and Avraham S 
In:
J Cell Sci (2005) 118(Pt 23): 5537-5548 
Ral GTPases regulate neurite branching through GAP-43 and the exocyst complex
Categories:
Transfection 
Authors:
Lalli G, Hall A 
In:
J Cell Biol (2005) 171(5): 857-69 
Distinct enzyme combinations in AKAP signalling complexes permit functional diversity
Categories:
Transfection 
Authors:
Hoshi N, Langeberg LK and Scott JD 
In:
Nat Cell Biol (2005) 7(11): 1066-1073 
A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis
Categories:
Transfection 
Authors:
Vo N, Klein ME, Varlamova O, Keller DM, Yamamoto T, Goodman RH and Impey S 
In:
Proc Natl Acad Sci USA (2005) 102(45): 16426-16431 
Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors
Categories:
Transfection 
Authors:
Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG and Moss SJ 
In:
J Neurosci (2005) 25(45): 10469-10478 
Sorting of vesicular monoamine transporter 2 to the regulated secretory pathway confers the somatodendritic exocytosis of monoamines
Categories:
Transfection 
Authors:
Li H, Waites CL, Staal RG, Dobryy Y, Park J, Sulzer DL, Edwards RH 
In:
Neuron (2005) 48(4): 619-33 
Coupling between NMDA receptor and acid-sensing ion channel contributes to ischemic neuronal death
Categories:
Transfection 
Authors:
Gao J, Duan B, Wang DG, Deng XH, Zhang GY, Xu L, Xu TL 
In:
Neuron (2005) 48(4): 635-46 
Association of an AKAP Signaling Scaffold with Cadherin Adhesion Molecules in Neurons and Epithelial Cells
Categories:
Transfection 
Authors:
Gorski JA, Gomez LL, Scott JD and Dell'acqua ML 
In:
Mol Biol Cell (2005) 16(8): 3574-3590 
Par-4 links dopamine signaling and depression
Categories:
Transfection 
Authors:
Park SK, Nguyen MD, Fischer A, Luke MP, Affar el B, Dieffenbach PB, Tseng HC, Shi Y and Tsai LH 
In:
Cell (2005) 122(2): 275-287 
Regulation of microtubule severing by katanin subunits during neuronal development
Categories:
Transfection 
Authors:
Yu W, Solowska JM, Qiang L, Karabay A, Baird D and Baas PW 
In:
J Neurosci (2005) 25(23): 5573-5583 
Sortilin controls intracellular sorting of brain-derived neurotrophic factor to the regulated secretory pathway
Categories:
Transfection 
Authors:
Chen ZY, Ieraci A, Teng H, Dall H, Meng CX, Herrera DG, Nykjaer A, Hempstead BL and Lee FS 
In:
J Neurosci (2005) 25(26): 6156-6166 
Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor
Categories:
Transfection 
Authors:
Li Y, Jia YC, Cui K, Li N, Zheng ZY, Wang YZ and Yuan XB 
In:
Nature (2005) 434(7035): 894-898 
Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments
Categories:
Transfection 
Authors:
He Y, Francis F, Myers KA, Yu W, Black MM and Baas PW 
In:
J Cell Biol (2005) 168(5): 697-703 
Critical Role of Collapsin Response Mediator Protein-associated Molecule CRAM for Filopodia and Growth Cone Development in Neurons
Categories:
Transfection 
Authors:
Hotta A, Inatome R, Yuasa-Kawada J, Qin Q, Yamamura H and Yanagi S 
In:
Mol Biol Cell (2005) 16(1): 32-39 
Both the establishment and the maintenance of neuronal polarity require active mechanisms: critical roles of GSK-3beta and its upstream regulators
Categories:
Transfection 
Authors:
Jiang H, Guo W, Liang X and Rao Y 
In:
Cell (2005) 120(1): 123-135 
Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons
Categories:
Transfection 
Authors:
Miao HS, Yu LY, Hui GZ and Guo LH 
In:
Acta Pharmacol Sin (2005) 26(1): 33-8 
Morphine induces terminal micro-opioid receptor desensitization by sustained phosphorylation of serine-375
Categories:
Transfection 
Authors:
Schulz S, Mayer D, Pfeiffer M, Stumm R, Koch T and Hollt V 
In:
EMBO J (2004) 23(16): 3282-3289 
Rapid vesicular translocation and insertion of TRP channels
Categories:
Transfection 
Authors:
Bezzerides VJ, Ramsey IS, Kotecha S, Greka A and Clapham DE 
In:
Nat Cell Biol (2004) 6(8): 709-720 
Huntingtin-associated protein 1 regulates inhibitory synaptic transmission by modulating gamma-aminobutyric acid type A receptor membrane trafficking
Categories:
Transfection 
Authors:
Kittler JT, Thomas P, Tretter V, Bogdanov YD, Haucke V, Smart TG and Moss SJ 
In:
Proc Natl Acad Sci USA (2004) 101(34): 12736-12741 
Mechanism of NF-kappaB inactivation induced by survival signal withdrawal in cerebellar granule neurons
Categories:
Transfection 
Authors:
Kovacs AD, Chakraborty-Sett S, Ramirez SH, Sniderhan LF, Williamson AL and Maggirwar SB 
In:
Eur J Neurosci (2004) 20(2): 345-352 
Axonal growth is sensitive to the levels of katanin, a protein that severs microtubules
Categories:
Transfection 
Authors:
Karabay A, Yu W, Solowska JM, Baird DH and Baas PW 
In:
J Neurosci (2004) 24(25): 5778-5788 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Efficient Transfection Method for Rat Primary Hippocampal Neurons
Categories:
Transfection 
Authors:
Miao HS, Yu LY, Lin B, Wu JC, Guo LH and Hui GZ 
In:
Chin J Cell Biol (2004) 26(3): 305-308 
Mitogen- and stress-activated protein kinase 1 mediates cAMP response element-binding protein phosphorylation and activation by neurotrophins.
Categories:
Transfection 
Authors:
Arthur JS, Fong AL, Dwyer JM, Davare M, Reese E, Obrietan K and Impey S 
In:
J Neurosci (2004) 24(18): 4324-32 
Marlin-1 a novel RNA binding protein associates with GABA(subB) receptors
Categories:
Transfection 
Authors:
Couve A, Restituito S, Brandon JM, Charles KJ, Bawagan H, Freeman KB, Pangalos MN, Calver AR and Moss SJ 
In:
J Biol Chem (2004) 279(14): 13934-13943 
The X-linked mental retardation protein oligophrenin-1 is required for dendritic spine morphogenesis
Categories:
Transfection 
Authors:
Govek EE, Newey SE, Akerman CJ, Cross JR, Van der Veken L and Van Aelst L 
In:
Nat Neurosci (2004) 7(4): 364-372 
LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex
Categories:
Transfection 
Authors:
Mi S, Lee X, Shao Z, Thill G, Ji B, Relton J, Levesque M, Allaire N, Perrin S, Sands B, Crowell T, Cate RL, McCoy JM and Pepinsky RB 
In:
Nat Neurosci (2004) 7(3): 221-8 
Nurr1-RXR heterodimers mediate RXR ligand-induced signaling in neuronal cells
Categories:
Transfection 
Authors:
Wallen-Mackenzie A, De Urquiza AM, Petersson S, Rodriguez FJ, Friling S, Wagner J, Ordentlich P, Lengqvist J, Heyman RA, Arenas E and Perlmann T 
In:
Genes Dev (2003) 17(24): 3036-3047 
ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type I gamma
Categories:
Transfection 
Authors:
Krauss M, Kinuta M, Wenk MR, De Camilli P, Takei K and Haucke V 
In:
J Cell Biol (2003) 162(1): 113-124 
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