Polymer peel-off mask for high-resolution surface derivatization, neuron placement and guidance

Authors:
Martinez D, Py C, Denhoff M, Monette R, Comas T, Krantis A, Mealing G
In:
Source: Biotechnol Bioeng
Publication Date: (2013)
Issue: 110(8): 2236-41
Research Area:
Neurobiology
Basic Research
Cells used in publication:
Neuron, hippo/cortical, rat
Species: rat
Tissue Origin: brain
Dorsal root gang. (DRG), rat
Species: rat
Tissue Origin: brain
Abstract
We present a dry lift-off method using a chemically resistant spin-on plastic, polyimide, to pattern surfaces with high accuracy and resolution. Using well-known lithographic and reactive ion etching techniques, the spin-on polymer is patterned over a silicon dioxide surface. The plastic efficiently adheres to the silicon dioxide surface during the chemical modification and is readily lifted-off following the derivatization process, permitting highly reliable surface derivatization. The verticality of the reactive ion etch enables sub-micrometer features to be patterned, down to 0.8?µm. The technique is used to pattern neurons on silicon dioxide surfaces: efficient neuron placement over a 4?mm area is shown for patterns larger than 50?µm while process guidance is shown for 10?µm patterns