Dorsal root gang. (DRG), rat

A collection of neurons associated with the processing of sensory information. Found within the sensory portion of the spinal nerve, just as the nerve exits the spinal foramina.

Cell Type:
Neurons (Ganglia)
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, rat O-003 2e6 33-49% Plasmid (general) 3 µg 100 µl I/II/2b

Citations (45)

Categories:
Primary Cells and Media 
Authors:
Katrien Pletinckx , Duygu Krings , André Welbers , David A Rider , Torsten R Dunkern 
In:
Brain Behav Immun. (2020) :  
Categories:
Primary Cells and Media 
Authors:
Geohagen BC, Weiser DA, Loeb DM, Nordstroem LU, LoPachin RM 
In:
Chem Biol (2020) 317 : 108961 
Categories:
Primary Cells and Media 
Authors:
Namchaiw P WEn H Mayrhofer F Chechneva O Biswas S Deng W 
In:
Stem Cell Res (2019) 10: 1 
Categories:
Primary Cells and Media 
Authors:
Datta G, Miller NM, Afghah Z, Geiger JD, Chen X 
In:
Front Neurosci (2019) 13: 329 
Categories:
Primary Cells and Media 
Authors:
Biswas S Chung SH Jiang P Dhaghan S Deng W 
In:
Scientific Reports (2019) 9: 1 
Categories:
Primary Cells and Media 
Authors:
D\\\'Este E, Kamin D, Velte C, Göttfert F, Simons M, Hell SW 
In:
Scientific Reports (2016) 7(6): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
De Nadai T, Marchetti L, Di Rienzo C, Calvello M, Signore G3, Di Matteo P, Gobbo F, Turturro S, Meucci S, Viegi A, Beltram F, Luin S, Cattaneo A 
In:
Scientific Reports (2016) 1(6): ePub 
Categories:
Primary Cells and Media 
Authors:
Kumamoto J, Kitahata H, Goto M, Nagayama M, Denda M 
In:
Biochem Biophys Res Commun (2015) ePub: ePub 
Categories:
Primary Cells and Media 
Authors:
Wu SN, So EC, Liao YK, Huang YM 
In:
Pain (2015) 16(5): 1032-1034 
Categories:
 
Authors:
Patel R, Rutten K, Valdor M, Schiene K, Wigge S, Schunk S, Damann N, Christoph T, Dickenson AH3 
In:
Neuroscience (2015) ePub: ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tian H, Li CS, Scott TP, Montgomery SR, Phan K, Lao L, Zhang W, Li Y, Hayashi T, Takahashi S, Alobaidaan R, Ruangchainikom M, Zhao KW4, Brochmann EJ, Murray SS, Wang JC, Daubs MD. 
In:
Spine J (2014) 25: ePub 
Categories:
Primary Cells and Media 
Authors:
Martinez D, Py C, Denhoff M, Monette R, Comas T, Krantis A, Mealing G 
In:
Biotechnol Bioeng (2013) 110(8): 2236-41 
Categories:
Primary Cells and Media 
Authors:
Ikeyama K, Nakatani M, Kumamoto J, Denda M 
In:
Skin Research & Technology (2013) 19(3): 346-51 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao L, Xun J, Jiang X, Tan R 
In:
Other (2013) 68(8): 677-80 
Categories:
Primary Cells and Media 
Authors:
Liu H, Lu M, Guthrie KM 
In:
Experimental Neurology (2013) 241: 125-37 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Muffley LA, Pan SC, Smith AN, Ga M, Hocking AM, Gibran NS. 
In:
Experimental Cell Research (2012) 318(16): 2085-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Thoma EC, Merkl C, Heckel T, Haab R, Knoflach F, Nowaczyk C, Flint N, Jagasia R, Zoffmann SJ, Truong HH, Petitjean P, Jessberger S, Graf M, Iacone R 
In:
Other (2012) 3: 1-9 
Categories:
Transfection 
Authors:
Ritter DM, Ho C, O\\\'Leary ME, Covarrubias M. 
In:
J Physiol (2012) 590(Pt 1): 145-61 
Categories:
Primary Cells and Media 
Authors:
Tominaga M, Tengara S, Kamo A, Ogawa H, Takamori K. 
In:
J Invest Dermatol (2011) 131(10): 2105-12 
Categories:
Primary Cells and Media 
Authors:
Tsutsumi M, Goto M, Denda S, Denda M. 
In:
Exp Dermatol (2011) 20(6): 464-7 
Categories:
Primary Cells and Media 
Authors:
Antinone SE, Zaichick SV, Smith GA. 
In:
J Virol (2010) 84(24): 13019-30 
Categories:
Primary Cells and Media 
Authors:
Abbadie C, McManus OB, Sun SY, Bugianesi RM, Dai G, Haedo RJ, Herrington JB, Kaczorowski GJ, Smith MM, Swensen AM, Warren VA, Williams B, Arneric SP, Eduljee C, Snutch TP, Tringham EW, Jochnowitz N, Liang A, Euan MacIntyre D, McGowan E, Mistry S, White VV, Hoyt SB, London C, Lyons KA, Bunting PB, Volksdorf S, Duffy JL. 
In:
J Pharmacol Exp Ther (2010) 334(2): 545-55 
Categories:
Primary Cells and Media 
Authors:
Tengara S, Tominaga M, Kamo A, Taneda K, Negi O, Ogawa H, Takamori K. 
In:
J Dermatol Sci (2010) 58(1):: 64-71 
Categories:
Primary Cells and Media 
Authors:
Taneda K, Tominaga M, Tengara S, Ogawa H, Takamori K. 
In:
Clin Exp Dermatol (2010) 35(1): 73-7 
Categories:
Primary Cells and Media 
Authors:
Tominaga M, Kamo A, Tengara S, Ogawa H, Takamori K. 
In:
Br J Pharmacol (2009) 161(5): 1028-37 
Categories:
Primary Cells and Media 
Authors:
Riera CE, Menozzi-Smarrito C, Affolter M, Michlig S, Munari C, Robert F, Vogel H, Simon SA, le Coutre J. 
In:
Br J Pharmacol (2009) 157(8): 1398-409 
Categories:
Primary Cells and Media 
Authors:
Gruber JV, Holtz R. 
In:
Toxicol Ind Health (2009) 25(4-5): 225-30 
Categories:
Primary Cells and Media 
Authors:
Carlin KP, Bui TV, Dai Y, Brownstone RM. 
In:
J Neurosci (2009) 29(16): 5343-53 
Categories:
Transfection 
Authors:
Seggio AM, Ellison KS, Hynd MR, Shain W, Thompson DM 
In:
J Neurosci Methods (2008) 173(1): 67-73 
Categories:
Primary Cells and Media 
Authors:
Anesti AM, Peeters PJ, Royaux I, Coffin RS. 
In:
Nucleic Acids Res (2008) 36(14): e86 
Categories:
Transfection 
Authors:
Inoue KI, Ito K, Osato M, Lee B, Bae SC, Ito Y 
In:
J Biol Chem (2007) 282(33): 24175-84 
Categories:
Primary Cells and Media 
Authors:
Riera CE, Vogel H, Simon SA, le Coutre J. 
In:
Am J Physiol Regul Integr Comp Physiol (2007) 293(2): R626-34 
Categories:
Transfection 
Authors:
Dzhashiashvili Y, Zhang Y, Galinska J, Lam I, Grumet M, Salzer JL 
In:
J Cell Biol (2007) 177(5): 857-70 
Categories:
Primary Cells and Media 
Authors:
Murashov AK, Chintalgattu V, Islamov RR, Lever TE, Pak ES, Sierpinski PL, Katwa LC, Van Scott MR. 
In:
FEBS J (2007) 21(3): 656-70 
Categories:
Transfection 
Authors:
Gatlin JC, Estrada-Bernal A, Sanford SD, Pfenninger KH 
In:
Mol Biol Cell (2006) 17(12): 5115-30 
Categories:
Transfection 
Authors:
Harty TP, Dib-Hajj SD, Tyrrell L, Blackman R, Hisama FM, Rose JB, Waxman SG 
In:
J Neurosci (2006) 26(48): 12566-75 
Categories:
Transfection 
Authors:
Choi JS, Hudmon A, Waxman S, Dib-Hajj SD 
In:
J Neurophysiol (2006) 96(1): 97-108 
Categories:
Transfection 
Authors:
Rush AM, Dib-Hajj SD, Liu S, Cummins TR, Black JA, Waxman SG 
In:
Proc Natl Acad Sci USA (2006) 103(21): 8245-50 
Categories:
Transfection 
Authors:
Chadborn NH, Ahmed AI, Holt MR, Prinjha R, Dunn GA, Jones GE, Eickholt BJ 
In:
J Cell Sci (2006) 119(Pt 5): 951-7 
Categories:
Transfection 
Authors:
Hood C, Cunningham AL, Slobedman B, Arvin AM, Sommer MH, Kinchington PR and Abendroth A 
In:
J Virol (2006) 80(2): 1025-1031 
Categories:
Transfection 
Authors:
Taveggia C, Zanazzi G, Petrylak A, Yano H, Rosenbluth J, Einheber S, Xu X, Esper RM, Loeb JA, Shrager P, Chao MV, Falls DL, Role L and Salzer JL 
In:
Neuron (2005) 47(5): 681-694 
Categories:
Transfection 
Authors:
Domeniconi M, Zampieri N, Spencer T, Hilaire M, Mellado W, Chao MV and Filbin MT 
In:
Neuron (2005) 46(6): 849-855 
Categories:
Transfection 
Authors:
Leclere PG, Panjwani A, Docherty R, Berry M, Pizzey J, Tonge DA 
In:
J Neurosci Methods (2005) 142(1): 137-143 
Categories:
Transfection 
Authors:
Brown M, Jacobs T, Eickholt B, Ferrari G, Teo M, Monfries C, Qi RZ, Leung T, Lim L and Hall C 
In:
J Neurosci (2004) 24(41): 8994-9004 
Categories:
Transfection 
Authors:
Viard P, Butcher AJ, Halet G, Davies A, Nurnberg B, Heblich F and Dolphin AC 
In:
Nat Neurosci (2004) 7(9): 939-946 
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