Dorsal root gang. (DRG), rat

Export

A collection of neurons associated with the processing of sensory information. Found within the sensory portion of the spinal nerve, just as the nerve exits the spinal foramina.

Cell Type:
Neurons (Ganglia)
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, rat O-003 2e6 33-49% Plasmid (general) 3 µg 100 µl I/II/2b

Citations (46)

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers
Categories:
Primary Cells and Media, Transfection 
Authors:
Derek P Wong , Nand K Roy , Keman Zhang , Anusha Anukanth, Abhishek Asthana , Nicole J Shirkey-Son , Samantha Dunmire , Bryan J Jones , Walker S Lahr , Beau R Webber , Branden S Moriarity , Paolo Caimi , Reshmi Parameswaran  
In:
Nat Commun. (2022) 13: 217 
Central IRAK-4 kinase inhibition for the treatment of pain following nerve injury in rats
Categories:
Primary Cells and Media 
Authors:
Katrien Pletinckx , Duygu Krings , André Welbers , David A Rider , Torsten R Dunkern 
In:
Brain Behav Immun. (2020) :  
Enolate-forming compounds provide protection from platinum neurotoxicity
Categories:
Primary Cells and Media 
Authors:
Geohagen BC, Weiser DA, Loeb DM, Nordstroem LU, LoPachin RM 
In:
Chem Biol (2020) 317 : 108961 
Temporal and partial inhibition of GLI1 in neural stem cells (NSCs) results in the early maturation of NSC derived oligodendrocytes in vitro.
Categories:
Primary Cells and Media 
Authors:
Namchaiw P WEn H Mayrhofer F Chechneva O Biswas S Deng W 
In:
Stem Cell Res (2019) 10: 1 
HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells
Categories:
Primary Cells and Media 
Authors:
Datta G, Miller NM, Afghah Z, Geiger JD, Chen X 
In:
Front Neurosci (2019) 13: 329 
Development of glial restricted human neural stem cells for oligodendrocyte differentiation in vitro and in vivo.
Categories:
Primary Cells and Media 
Authors:
Biswas S Chung SH Jiang P Dhaghan S Deng W 
In:
Scientific Reports (2019) 9: 1 
Subcortical cytoskeleton periodicity throughout the nervous system
Categories:
Primary Cells and Media 
Authors:
D\\\'Este E, Kamin D, Velte C, Göttfert F, Simons M, Hell SW 
In:
Scientific Reports (2016) 7(6): ePub 
Precursor and mature NGF live tracking: one versus many at a time in the axons.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
De Nadai T, Marchetti L, Di Rienzo C, Calvello M, Signore G3, Di Matteo P, Gobbo F, Turturro S, Meucci S, Viegi A, Beltram F, Luin S, Cattaneo A 
In:
Scientific Reports (2016) 1(6): ePub 
Effects of medium flow on axon growth with or without nerve growth factor.
Categories:
Primary Cells and Media 
Authors:
Kumamoto J, Kitahata H, Goto M, Nagayama M, Denda M 
In:
Biochem Biophys Res Commun (2015) ePub: ePub 
Reversal by ranolazine of doxorubicin-induced prolongation in the inactivation of late sodium current in rat dorsal root ganglion neurons
Categories:
Primary Cells and Media 
Authors:
Wu SN, So EC, Liao YK, Huang YM 
In:
Pain (2015) 16(5): 1032-1034 
Electrophysiological characterization of activation state-dependent Cav2 channel antagonist TROX-1 in spinal nerve injured rats.
Categories:
 
Authors:
Patel R, Rutten K, Valdor M, Schiene K, Wigge S, Schunk S, Damann N, Christoph T, Dickenson AH3 
In:
Neuroscience (2015) ePub: ePub 
Secreted phosphoprotein 24 kD (Spp24) inhibits nerve root inflammation induced by bone morphogenetic protein-2.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tian H, Li CS, Scott TP, Montgomery SR, Phan K, Lao L, Zhang W, Li Y, Hayashi T, Takahashi S, Alobaidaan R, Ruangchainikom M, Zhao KW4, Brochmann EJ, Murray SS, Wang JC, Daubs MD. 
In:
Spine J (2014) 25: ePub 
Polymer peel-off mask for high-resolution surface derivatization, neuron placement and guidance
Categories:
Primary Cells and Media 
Authors:
Martinez D, Py C, Denhoff M, Monette R, Comas T, Krantis A, Mealing G 
In:
Biotechnol Bioeng (2013) 110(8): 2236-41 
Distinct intracellular calcium responses of individual cultured human keratinocytes to air pressure changes.
Categories:
Primary Cells and Media 
Authors:
Ikeyama K, Nakatani M, Kumamoto J, Denda M 
In:
Skin Research & Technology (2013) 19(3): 346-51 
Propofol up-regulates Mas receptor expression in dorsal root ganglion neurons.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao L, Xun J, Jiang X, Tan R 
In:
Other (2013) 68(8): 677-80 
Anterograde trafficking of neurotrophin-3 in the adult olfactory system in vivo.
Categories:
Primary Cells and Media 
Authors:
Liu H, Lu M, Guthrie KM 
In:
Experimental Neurology (2013) 241: 125-37 
Differentiation state determines neural effects on microvascular endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Muffley LA, Pan SC, Smith AN, Ga M, Hocking AM, Gibran NS. 
In:
Experimental Cell Research (2012) 318(16): 2085-93 
Chemical Conversion of Human Fibroblasts into Functional Schwann Cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Thoma EC, Merkl C, Heckel T, Haab R, Knoflach F, Nowaczyk C, Flint N, Jagasia R, Zoffmann SJ, Truong HH, Petitjean P, Jessberger S, Graf M, Iacone R 
In:
Other (2012) 3: 1-9 
Modulation of Kv3.4 channel N-type inactivation by protein kinase C shapes the action potential in dorsal root ganglion neurons.
Categories:
Transfection 
Authors:
Ritter DM, Ho C, O'Leary ME, Covarrubias M. 
In:
J Physiol (2012) 590(Pt 1): 145-61 
Matrix metalloproteinase-8 is involved in dermal nerve growth: implications for possible application to pruritus from in vitro models
Categories:
Primary Cells and Media 
Authors:
Tominaga M, Tengara S, Kamo A, Ogawa H, Takamori K. 
In:
J Invest Dermatol (2011) 131(10): 2105-12 
Morphological and functional differences in coculture system of keratinocytes and dorsal-root-ganglion-derived cells depending on time of seeding
Categories:
Primary Cells and Media 
Authors:
Tsutsumi M, Goto M, Denda S, Denda M. 
In:
Exp Dermatol (2011) 20(6): 464-7 
Resolving the assembly state of herpes simplex virus during axon transport by live-cell imaging
Categories:
Primary Cells and Media 
Authors:
Antinone SE, Zaichick SV, Smith GA. 
In:
J Virol (2010) 84(24): 13019-30 
Analgesic effects of a substituted N-triazole oxindole (TROX-1), a state-dependent, voltage-gated calcium channel 2 blocker.
Categories:
Primary Cells and Media 
Authors:
Abbadie C, McManus OB, Sun SY, Bugianesi RM, Dai G, Haedo RJ, Herrington JB, Kaczorowski GJ, Smith MM, Swensen AM, Warren VA, Williams B, Arneric SP, Eduljee C, Snutch TP, Tringham EW, Jochnowitz N, Liang A, Euan MacIntyre D, McGowan E, Mistry S, White VV, Hoyt SB, London C, Lyons KA, Bunting PB, Volksdorf S, Duffy JL. 
In:
J Pharmacol Exp Ther (2010) 334(2): 545-55 
Keratinocyte-derived anosmin-1, an extracellular glycoprotein encoded by the X-linked Kallmann syndrome gene, is involved in modulation of epidermal nerve density in atopic dermatitis
Categories:
Primary Cells and Media 
Authors:
Tengara S, Tominaga M, Kamo A, Taneda K, Negi O, Ogawa H, Takamori K. 
In:
J Dermatol Sci (2010) 58(1):: 64-71 
Neurotropin inhibits both capsaicin-induced substance P release and nerve growth factor-induced neurite outgrowth in cultured rat dorsal root ganglion neurones
Categories:
Primary Cells and Media 
Authors:
Taneda K, Tominaga M, Tengara S, Ogawa H, Takamori K. 
In:
Clin Exp Dermatol (2010) 35(1): 73-7 
In vitro model for penetration of sensory nerve fibres on a Matrigel basement membrane: implications for possible application to intractable pruritus
Categories:
Primary Cells and Media 
Authors:
Tominaga M, Kamo A, Tengara S, Ogawa H, Takamori K. 
In:
Br J Pharmacol (2009) 161(5): 1028-37 
Compounds from Sichuan and Melegueta peppers activate, covalently and non-covalently, TRPA1 and TRPV1 channels.
Categories:
Primary Cells and Media 
Authors:
Riera CE, Menozzi-Smarrito C, Affolter M, Michlig S, Munari C, Robert F, Vogel H, Simon SA, le Coutre J. 
In:
Br J Pharmacol (2009) 157(8): 1398-409 
Examining communication between ultraviolet (UV)-damaged cutaneous nerve cells and epidermal keratinocytes in vitro.
Categories:
Primary Cells and Media 
Authors:
Gruber JV, Holtz R. 
In:
Toxicol Ind Health (2009) 25(4-5): 225-30 
Staircase currents in motoneurons: insight into the spatial arrangement of calcium channels in the dendritic tree
Categories:
Primary Cells and Media 
Authors:
Carlin KP, Bui TV, Dai Y, Brownstone RM. 
In:
J Neurosci (2009) 29(16): 5343-53 
Cryopreservation of transfected primary dorsal root ganglia neurons
Categories:
Transfection 
Authors:
Seggio AM, Ellison KS, Hynd MR, Shain W, Thompson DM 
In:
J Neurosci Methods (2008) 173(1): 67-73 
Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus
Categories:
Primary Cells and Media 
Authors:
Anesti AM, Peeters PJ, Royaux I, Coffin RS. 
In:
Nucleic Acids Res (2008) 36(14): e86 
The transcription factor Runx3 represses the neurotrophin receptor TrkB during lineage commitment of dorsal root ganglion neurons
Categories:
Transfection 
Authors:
Inoue KI, Ito K, Osato M, Lee B, Bae SC, Ito Y 
In:
J Biol Chem (2007) 282(33): 24175-84 
Artificial sweeteners and salts producing a metallic taste sensation activate TRPV1 receptors
Categories:
Primary Cells and Media 
Authors:
Riera CE, Vogel H, Simon SA, le Coutre J. 
In:
Am J Physiol Regul Integr Comp Physiol (2007) 293(2): R626-34 
Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms
Categories:
Transfection 
Authors:
Dzhashiashvili Y, Zhang Y, Galinska J, Lam I, Grumet M, Salzer JL 
In:
J Cell Biol (2007) 177(5): 857-70 
RNAi pathway is functional in peripheral nerve axons.
Categories:
Primary Cells and Media 
Authors:
Murashov AK, Chintalgattu V, Islamov RR, Lever TE, Pak ES, Sierpinski PL, Katwa LC, Van Scott MR. 
In:
FEBS J (2007) 21(3): 656-70 
Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding
Categories:
Transfection 
Authors:
Gatlin JC, Estrada-Bernal A, Sanford SD, Pfenninger KH 
In:
Mol Biol Cell (2006) 17(12): 5115-30 
Na(V)1.7 mutant A863P in erythromelalgia: effects of altered activation and steady-state inactivation on excitability of nociceptive dorsal root ganglion neurons
Categories:
Transfection 
Authors:
Harty TP, Dib-Hajj SD, Tyrrell L, Blackman R, Hisama FM, Rose JB, Waxman SG 
In:
J Neurosci (2006) 26(48): 12566-75 
Calmodulin regulates current density and frequency-dependent inhibition of sodium channel Nav1.8 in DRG neurons
Categories:
Transfection 
Authors:
Choi JS, Hudmon A, Waxman S, Dib-Hajj SD 
In:
J Neurophysiol (2006) 96(1): 97-108 
A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons
Categories:
Transfection 
Authors:
Rush AM, Dib-Hajj SD, Liu S, Cummins TR, Black JA, Waxman SG 
In:
Proc Natl Acad Sci USA (2006) 103(21): 8245-50 
PTEN couples Sema3A signalling to growth cone collapse
Categories:
Transfection 
Authors:
Chadborn NH, Ahmed AI, Holt MR, Prinjha R, Dunn GA, Jones GE, Eickholt BJ 
In:
J Cell Sci (2006) 119(Pt 5): 951-7 
Varicella-Zoster Virus ORF63 Inhibits Apoptosis of Primary Human Neurons
Categories:
Transfection 
Authors:
Hood C, Cunningham AL, Slobedman B, Arvin AM, Sommer MH, Kinchington PR and Abendroth A 
In:
J Virol (2006) 80(2): 1025-1031 
Neuregulin-1 Type III Determines the Ensheathment Fate of Axons
Categories:
Transfection 
Authors:
Taveggia C, Zanazzi G, Petrylak A, Yano H, Rosenbluth J, Einheber S, Xu X, Esper RM, Loeb JA, Shrager P, Chao MV, Falls DL, Role L and Salzer JL 
In:
Neuron (2005) 47(5): 681-694 
MAG Induces Regulated Intramembrane Proteolysis of the p75 Neurotrophin Receptor to Inhibit Neurite Outgrowth
Categories:
Transfection 
Authors:
Domeniconi M, Zampieri N, Spencer T, Hilaire M, Mellado W, Chao MV and Filbin MT 
In:
Neuron (2005) 46(6): 849-855 
Effective gene delivery to adult neurons by a modified form of electroporation
Categories:
Transfection 
Authors:
Leclere PG, Panjwani A, Docherty R, Berry M, Pizzey J, Tonge DA 
In:
J Neurosci Methods (2005) 142(1): 137-143 
Alpha2-chimaerin, cyclin-dependent Kinase 5/p35, and its target collapsin response mediator protein-2 are essential components in semaphorin 3A-induced growth-cone collapse
Categories:
Transfection 
Authors:
Brown M, Jacobs T, Eickholt B, Ferrari G, Teo M, Monfries C, Qi RZ, Leung T, Lim L and Hall C 
In:
J Neurosci (2004) 24(41): 8994-9004 
PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane
Categories:
Transfection 
Authors:
Viard P, Butcher AJ, Halet G, Davies A, Nurnberg B, Heblich F and Dolphin AC 
In:
Nat Neurosci (2004) 7(9): 939-946 
+ Show All

Privacy | Legal | About Lonza

© Lonza. All rights reserved.