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523 results sorted by
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A SingleQuots™ Supplements and Growth Factor Kit contains several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots - designed to be added to a bottle of basal medium. The concentrations of these factors are...
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For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000.
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We offer FlashGel™ Cassettes for DNA, DNA recovery and RNA separation. FlashGel™ DNA Cassettes are available at either 1.2% or 2.2% agarose, and in 12+1 single-tier (13 wells) or 16+1 double-tier formats (34 wells). FlashGel™ DNA Recovery Cassettes...
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We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual liquid...
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We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...
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We offer two different kit sizes for the 100 µl Nucleocuvette® Format (12 and 24 reactions) and one size for the 20 µl 16-well Nucleocuvette® Strip Format (32 reactions).
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Once you have found your optimal conditions you can always try to reduce the recommended cell number. To find out if lower cell numbers have been successfully used already, please check our cell data base. If you would like to reduce the cell number...
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The 4D-Nucleofector® System offers great flexibility in terms of cell numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high cell...
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The 4D-Nucleofector® X Unit offers flexible throughput from 1 to 16 samples. When working with 100 µl single Nucleocuvettes® you can process two vessels in parallel. However subsequent handling of more than 2 vessels is eneased via the option to...
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?
Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells getting...
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