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Do I need to add antibiotics to your Clonetics™ Media? What is GA-1000?

For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000.

Can I use a medium other than the one you offer to culture Clonetics® Cells?

We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...

What kit sizes are available for the 4D-Nucleofector® X Unit?

We offer two different kit sizes for the 100 µl Nucleocuvette® Format (12 and 24 reactions) and one size for the 20 µl 16-well Nucleocuvette® Strip Format (32 reactions).

What cell numbers can I transfect when using the 4D-Nucleofector® System?

The 4D-Nucleofector® System offers great flexibility in terms of cell numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high cell...

What is a Clonetics™ or Poietics™ Basal Medium?

The Clonetics™ and Poietics™ Basal Media are primarily composed of trace metals, amino acids, vitamins, salts, and water. The basal medium contains no growth factors or antibiotics and is not sufficient to support cell attachment and growth. To the...

How many samples can I transfect in parallel when using the 4D-Nucleofector® X Unit?

The 4D-Nucleofector® X Unit offers flexible throughput from 1 to 16 samples. When working with 100 µl single Nucleocuvettes® you can process two vessels in parallel. However subsequent handling of more than 2 vessels is eneased via the option to...

How can I best clean used dipping electrodes and store unused for later use? Can I wash used dipping electrodes in an ethanol bath?

After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used...

How many different primary cell kits are available for the 4D-Nucleofector® System?

In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell-type...

How can I find out if other 24-well culture plates than Greiner or Nunc are compatible to the dipping electrode array for adherent Nucleofection® using the 4D-Nucleofector® Y Unit?

Please find attached a list with all plate types that have been checked for compatibility so far. If your plate type is not on this list, please contact our Scientific Support Team we can investigate compatibility.

What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?

With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?

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