Q:

I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?

A:

We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.

After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used electrodes, we would suggest to fill the corresponding well positions of a 24-well plate with 350-400 µl 80% EtOH, insert the dipping electrode array for about 2 min and then store the array in a fresh 24-well culture plate or the blister package for later use of the unused electrodes.

Categories:
Transfection
Laboratory Instrumentation
Research Areas:
Neurobiology
Uncategorized