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Can I still use Clonetics®/Poietics® Basal Media if it froze/partially froze during shipping?

Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...

Can I use Nucleofection® Technology for transfecting Plasmodium falciparum?

Yes, but it depends on the platform. We would strongly recommend using the 4D-Nucleofector® or 96-well Shuttle™ System. The successful use of the 96-well Shuttle® System for transfection of P. falciparum has been published by Caro F, Miller...

Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?

Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...

Can I run gels other than FlashGel® Cassettes on the FlashGel® Dock?

No. The FlashGel® Cassettes and FlashGel® Dock are a single system, designed to work together. FlashGel® RNA and DNA Cassettes are not compatible with other chambers. The dock is not compatible with other gels.

Why can’t I obtain a Certificate of Analysis (COA) for a component within a SingleQuot™ Kit?

Lonza’s SingleQuot™ Kits contain various supplements and growth factors (such as fetal bovine serum, antibiotics, or hydrocortisone). While each supplement and growth factor will have its own catalog and lot number, each supplement and growth factor...

Where are Lonza’s aortic adventitial fibroblasts (AoAF) isolated from?

Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...

Where are Lonza’s lung fibroblasts (NHLF and DHLF) isolated from?

Lonza’s lung fibroblasts are not taken from any specific area of the lung but instead are taken from an area that appears to be in good shape and is non-hemorrhagic. The pleural membrane is removed and the peripheral tissue is used. Enzymatic...

Where are Lonza’s dermal fibroblasts (NHDF) isolated from?

Lonza’s offers dermal fibroblasts from both adult and neonatal donors. Lonza’s adult dermal fibroblasts (NHDF-Ad) are usually isolated from tissue obtained from elective cosmetic surgical procedures. Generally, these procedures are abdominoplasties...

Do I need a special license when using the Nucleofector® Technology for commercial purposes?

Each Nucleofector® Device (4D-Nucleofector® System, Nucleofector® 2b Device, HT Nucleofector® System) comes with a research license. Research license terms are included in the instrument manual. The research license is...

What is the electrode gap distance in your 100µL aluminum cuvettes with the blue lids, included in the Nucleofector® I/II/2b Kits?

The distance between electrodes is 2mm.

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