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I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...

How long will your Clonetics™ rat DRG's (dorsal root ganglion cells) remain viable in culture?

The cells can remain viable in culture for about 28 days.

I've had my Clonetics™ Cells in culture for a few months, they're no longer growing and don't look like healthy cells, what's wrong?

All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...

Do the Clonetics™ Rat and Mouse Astrocytes require an extracellular matrix for plating?

No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

What age are the rats that your Clonetics™ Rat DRGs are isolated from?

The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

Will the GFAP expression in your Clonetics™ Normal Human Astrocytes remain the same throughout the culture?

The GFAP expression decreases rapidly with passaging. The results that we give are performed in the 1st passage out of cryopreservation.

What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

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