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What are Lonza's human Mesenchymal Stem Cells (PT-2501) cryopreserved in?

The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)

When should I use the MycoAlert® Assay Control Set?

The positive control should be always included as reference for a positive signal. It serves as control for the performance of the MycoAlert® Assay and the MycoAlert® PLUS Assay and the luminescence measurement.

According to which standard is the Nucleofector® System certified?

The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.

Is it typical for the fluorescence observed from my tagged protein to appear less than the empty fluorescent vector?

Yes, this is a routine observation.Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.

Can I expand the mononuclear cells, CD34+ progenitors, without differentiating them?

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.

In my Nucleofection® Experiment, how critical is the time point for analysis post transfection?

It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...

Can Lonza's Human Adipose-Derived Stem Cells (hADSC) (PT-5006) be expanded prior to differentiation?

Lonza's human adipose derived stem cells (ADSC) are guarantee 5 passages prior to differentiation based on our existing data.

Do we sell the Nucleofector® Solution (or any other component of the Nucleofector® Kit) separately?

We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.

What is a BulletKit® Media?

A BulletKit® Media contains a bottle of basal medium and a SingleQuots® Kit. It is possible to purchase each of these components separately. Once the basal medium is supplemented with the SingleQuots® Kit, it becomes complete growth medium.
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