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524 results sorted by
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Healthy cells in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels. Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number of...
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The LGM-3™ Medium contains human recombinant insulin, human albumin, and human transferrin.
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Yes. Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture. In response to a proliferative signal (e.g. a growth factor) the number of cells in a...
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To achieve optimal performance and convenience a microplate luminometer should be used. If reagent injectors are available on the instrument, these should be used since they enable full automation of the assay. If reagent injectors are...
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The lysis of the cells with the Cell Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible to...
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No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.
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There should be no problem with the use of the ViaLight™ Plus Kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...
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Yes. Please click on the link below
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Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...
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Certain compounds will quench the light emission in bioluminescent reactions. Typically these are strong red dyes (however, the ViaLight ™ Plus Assay has been designed to function in all phenol red containing media commonly used in...
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