Data Type
Cell Information
(857)
Citations
(4966)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
a
r
e
S
i
n
g
l
e
Q
u
o
t
s
™
K
i
t
s
?
A SingleQuots™ Supplements and Growth Factor Kit contains several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots - designed to be added to a bottle of basal medium. The concentrations of these factors are...
D
o
I
n
e
e
d
t
o
a
d
d
a
n
t
i
b
i
o
t
i
c
s
t
o
y
o
u
r
C
l
o
n
e
t
i
c
s
™
M
e
d
i
a
?
W
h
a
t
i
s
G
A
-
1
0
0
0
?
For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000.
A
t
w
h
a
t
v
o
l
t
a
g
e
s
h
o
u
l
d
I
r
u
n
a
n
a
g
a
r
o
s
e
g
e
l
?
We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...
H
o
w
m
a
n
y
d
i
f
f
e
r
e
n
t
p
r
i
m
a
r
y
c
e
l
l
k
i
t
s
a
r
e
a
v
a
i
l
a
b
l
e
f
o
r
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
?
In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell-type...
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
u
s
i
n
g
c
o
n
d
u
c
t
i
v
e
p
o
l
y
m
e
r
a
s
e
l
e
c
t
r
o
d
e
m
a
t
e
r
i
a
l
f
o
r
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
X
-
U
n
i
t
a
n
d
9
6
-
w
e
l
l
U
n
i
t
?
With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?
W
h
a
t
i
s
a
G
T
G
™
A
g
a
r
o
s
e
?
GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...
D
o
I
n
e
e
d
t
o
p
u
r
c
h
a
s
e
a
s
p
e
c
i
a
l
c
h
a
m
b
e
r
t
o
u
s
e
R
e
l
i
a
n
t
™
a
n
d
L
a
t
i
t
u
d
e
™
P
r
e
c
a
s
t
G
e
l
s
?
Reliant™ and Latitude™ Precast Agarose Gels are designed to run in standard horizontal electrophoresis chambers.As long as there is room on the chamber platform for the gel, the chamber should be suitable. Measure the chamber platform and check...
H
o
w
m
a
n
y
c
e
l
l
s
d
o
I
h
a
v
e
t
o
u
s
e
f
o
r
e
a
c
h
w
e
l
l
o
f
t
h
e
1
6
-
w
e
l
l
N
u
c
l
e
o
c
u
v
e
t
t
e
®
S
t
r
i
p
?
This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
D
o
I
n
e
e
d
t
o
s
h
a
k
e
t
h
e
p
l
a
t
e
a
f
t
e
r
a
d
d
i
n
g
t
h
e
C
e
l
l
L
y
s
i
s
R
e
a
g
e
n
t
d
u
r
i
n
g
t
h
e
V
i
a
L
i
g
h
t
™
A
s
s
a
y
?
No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
D
o
e
s
t
h
e
r
e
c
o
m
m
e
n
d
e
d
m
e
d
i
a
f
o
r
e
a
c
h
o
f
t
h
e
d
i
a
b
e
t
i
c
c
e
l
l
s
c
o
n
t
a
i
n
g
l
u
c
o
s
e
?
The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.
PREVIOUS
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
NEXT