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What is the difference between NuSieve 3:1 and NuSieve GTG Agaroses?

NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...

Can I reuse the 100µl Nucleocuvette® Vessel?

We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.

How can I best clean used dipping electrodes and store unused for later use? Can I wash used dipping electrodes in an ethanol bath?

After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used...

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

Can the ProSieve Molecular Weight Markers be used on native gels?

No, they are suitable for use on denaturing gels only.

I am using your ProSieve Color Protein Markers and the molecular weights (MW) of my protein of interest is not what I expected when compared to the ProSieve Markers. Why is this?

The proteins in our ProSieve™ Color Markers are made of proteins that are individually prestained using colored dyes.The level of dye bound can affect the migration of the protein and this can vary slightly lot to lot. Therefore, it is important to...

How is the B-ALI Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.
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