Data Type
Cell Information
(866)
Citations
(5015)
FAQ
(525)
Culture Media
(62)
Category
Primary Cells and Media
(201)
Transfection
(169)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(64)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
Classical Media and Reagents
(7)
3D Cell Culture
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(136)
Cancer Research/Cell Biology
(78)
Immunotherapy / Hematology
(62)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(41)
Neurobiology
(28)
Cardiovascular
(27)
Respiratory Research
(27)
Molecular Biology
(26)
Toxicology
(23)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(15)
Drug Discovery
(8)
Parasitology
(3)
Gastroenterology
(2)
+ Show All
525 results sorted by
relevance
alphabetical
newest first
oldest first
I
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
,
h
o
w
c
r
i
t
i
c
a
l
i
s
t
h
e
t
i
m
e
p
o
i
n
t
f
o
r
a
n
a
l
y
s
i
s
p
o
s
t
t
r
a
n
s
f
e
c
t
i
o
n
?
It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...
D
o
w
e
s
e
l
l
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
(
o
r
a
n
y
o
t
h
e
r
c
o
m
p
o
n
e
n
t
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
K
i
t
)
s
e
p
a
r
a
t
e
l
y
?
We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
F
o
l
l
o
w
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
®
,
I
c
a
n
n
o
t
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
f
r
o
m
s
i
n
g
l
e
c
e
l
l
s
.
W
h
a
t
c
o
u
l
d
b
e
t
h
e
r
e
a
s
o
n
?
Some cell lines need certain cell densities to proliferate. For those cells you can try using conditioned medium for setting up limiting dilution or culturing single cells together with feeder cells.
H
o
w
t
o
m
a
x
i
m
i
z
e
c
e
l
l
v
i
a
b
i
l
i
t
y
i
n
a
N
u
c
l
e
o
f
e
c
t
i
o
n
®
e
x
p
e
r
i
m
e
n
t
?
Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the cells at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the cells,...
W
h
a
t
a
n
t
i
b
i
o
t
i
c
c
o
n
c
e
n
t
r
a
t
i
o
n
s
h
o
u
l
d
I
u
s
e
f
o
r
s
e
l
e
c
t
i
n
g
s
t
a
b
l
e
t
r
a
n
s
f
e
c
t
a
n
t
s
?
We highly recommend to perform a titration for G418 in a range of 0,1 mg/ml to 1,5 mg/ml; in serum-free culture expand range down to 20 µg/ml).Choose the concentration which is 0,1 or 0,2 mg/ml above the one which shows complete cell death as...
W
h
a
t
i
s
t
h
e
f
u
n
c
t
i
o
n
o
f
r
e
t
i
n
o
i
c
a
c
i
d
i
n
t
h
e
B
E
G
M
®
m
e
d
i
a
?
In vivo, bronchial epithelial cells differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
W
h
a
t
a
r
e
m
y
c
o
p
l
a
s
m
a
s
?
Mycoplasmas belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "Mycoplasma" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct Mycoplasma...
H
o
w
m
u
c
h
D
N
A
s
h
o
u
l
d
I
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
R
e
a
c
t
i
o
n
t
o
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
?
You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our...
H
o
w
o
f
t
e
n
d
o
m
y
c
o
p
l
a
s
m
a
c
o
n
t
a
m
i
n
a
t
i
o
n
s
o
c
c
u
r
i
n
c
e
l
l
c
u
l
t
u
r
e
s
?
A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).
C
a
n
I
u
s
e
S
D
S
a
s
a
d
e
t
e
r
g
e
n
t
f
o
r
a
p
o
s
i
t
i
v
e
c
o
n
t
r
o
l
i
f
I
d
o
c
y
t
o
t
o
x
i
c
i
t
y
a
s
s
a
y
w
i
t
h
T
o
x
i
L
i
g
h
t
™
?
No. SDS has a negative influence on Adenylate Kinase (AK) activity. SDS is a cationic detergent, it is a very powerful protein denaturing agent, you will be aware of its use in SDS-PAGE for those reasons. ToxiLight™ works by measuring the activity of...
PREVIOUS
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
NEXT