Q:

How much DNA should I use in my Nucleofection™ Reaction to obtain stable clones?

A:
You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our "Guideline for Generation of Stable Cell Lines"
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Categories:
Transfection
Research Areas:
Uncategorized