Fibroblast Growth Medium-2

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Shelf Life:
12 months
Recommended for Cells:
Fibroblast, dermal (NHDF-Neo), human neonatal ; Fibroblast, dermal(NHDF-Ad), human adult ; Fibroblast, lung, human normal (NHLF) ; Fibroblast, lung (DHLF), human COPD ; Fibroblast, lung (DHFL), human asthma ; Fibroblast, lung (DHFL), human CF
Categories:
Primary Cells and Media
Research Areas:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Gene Expression
Basic Research
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Citations (36)

A microfluidic platform integrating functional vascularized organoids-on-chip
Categories:
3D Cell Culture , Serum-free and Speciality Media, Mycoplasma Detection and Prevention 
Authors:
Clément Quintard, Emily Tubbs , Gustav Jonsson , Jie Jiao , Jun Wang , Nicolas Werschler , Camille Laporte Amandine Pitaval , Thierno-Sidy Bah , Gideon Pomeranz , Caroline Bissardon , Joris Kaal , Alexandra Leopoldi , David A Long , Pierre Blandin , Jean-Luc Achard , Christophe Battail , Astrid Hagelkruys , Fabrice Navarro , Yves Fouillet , Josef M Penninger , Xavier Gidrol  
In:
Nature (2024) 15: 1 
NUDT21 limits CD19 levels through alternativemRNA polyadenylation in B cell acutelymphoblastic leukemia
Categories:
Primary Cells and Media, Transfection 
Authors:
Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, Aifantis I 
In:
Nature (2022) 10: 1424-1432 
3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections.
Categories:
Primary Cells and Media 
Authors:
Marrazzo P, Maccari S, Taddei A, Bevan L, Telford J, Soriani M, Pezzicoli A 
In:
PLoS ONE (2016) 11(4): e0153985 
Induction of heme oxygenase I (HMOX1) by HPP-4382: a novel modulator of Bach1 activity
Categories:
Primary Cells and Media 
Authors:
Attucks OC, Jasmer KJ, Hannink M, Kassis J, Zhong Z, Gupta S, Victory SF, Guzel M, Polisetti DR, Andrews R, Mjalli AM, Kostura MJ 
In:
PLoS ONE (2014) 9(7): e101044 
Arsenic trioxide inhibits transforming growth factor-ß1-induced fibroblast to myofibroblast differentiation in vitro and bleomycin induced lung fibrosis in vivo
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Luo F, Zhuang Y, Sides MD, Sanchez CG, Shan B, White ES, Lasky JA 
In:
Respir Res (2014) 24: 15:51 
Derivation of human differential photoreceptor cells from adult human dermal fibroblasts by defined combinations of CRX, RAX, OTX2 and NEUROD
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Seko Y1, Azuma N, Ishii T, Komuta Y, Miyamoto K, Miyagawa Y, Kaneda M, Umezawa A. 
In:
Genes Cells (2014) 19(3): 198-208 
Differential expression of tripartite motif-containing family in normal human dermal fibroblasts in response to porcine endogenous retrovirus infection
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimsa MW, Strzalka-Mrozik B, Kimsa MC, Mazurek U, Kruszniewska-Rajs C, Gola J, Adamska J, Twardoch M 
In:
Folia Biol (Praha) (2014) 60(3): 144-51 
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, Nakae S 
In:
PLoS ONE (2014) 9(1): e86106 
A small-molecule blocking ribonucleotide reductase holoenzyme formation inhibits cancer cell growth and overcomes drug resistance
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou B, Su L, Hu S, Hu W, Yip ML, Wu J, Gaur S, Smith DL, Yuan YC, Synold TW, Horne D, Yen Y 
In:
Cancer Res (2013) 73(21): 6484-93 
Cenderitide-eluting film for potential cardiac patch applications.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ng XW, Huang Y, Chen HH, Burnett JC Jr, Boey FY, Venkatraman SS. 
In:
PLoS ONE (2013) 8(7): e68346 
Senescent fibroblasts enhance early skin carcinogenic events via a paracrine MMP-PAR-1 axis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Malaquin N1, Vercamer C, Bouali F, Martien S, Deruy E, Wernert N, Chwastyniak M, Pinet F, Abbadie C, Pourtier A. 
In:
PLoS ONE (2013) 8(5): e63607 
Sphingosine 1-phosphate (S1P) receptor agonists mediate pro-fibrotic responses in normal human lung fibroblasts via S1P2 and S1P3 receptors and Smad-independent signaling
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sobel K, Menyhart K, Killer N, Renault B, Bauer Y, Studer R, Steiner B, Bolli MH, Nayler O, Gatfield J. 
In:
J Biol Chem (2013) 288(21): 14839-51 
Hellebrin and its aglycone form hellebrigenin display similar in vitro growth inhibitory effects in cancer cells and binding profiles to the alpha subunits of the Na+/K+-ATPase
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moreno Y Banuls L1, Katz A, Miklos W, Cimmino A, Tal DM, Ainbinder E, Zehl M, Urban E, Evidente A, Kopp B, Berger W, Feron O, Karlish S, Kiss R. 
In:
Mol Cancer (2013) 12: 33 
Production of a human neutralizing monoclonal antibody and its crystal structure in complex with ectodomain 3 of the interleukin-13 receptor a1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Redpath NT, Xu Y, Wilson NJ, Fabri LJ, Baca M, Andrews AE, Braley H, Lu P, Ireland C, Ernst RE, Woods A, Forrest G, An Z, Zaller DM, Strohl WR, Luo CS, Czabotar PE, Garrett TP, Hilton DJ, Nash AD, Zhang JG, Nicola NA. 
In:
Biochem J (2013) 451(2): 165-75 
Increased Expression of RhoA in Epithelium and Smooth Muscle of Obese Mouse Models: Implications for Isoprenoid Control of Airway Smooth Muscle and Fibroblasts
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ross KR1, Darrah RJ, Hodges CA, Lang L, Kelley TJ. 
In:
Journal of Allergy (2013) 2013: 740973 
Mitochondrial reactive oxygen species regulate transforming growth factor-ß signaling
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jain M, Rivera S, Monclus EA, Synenki L, Zirk A, Eisenbart J, Feghali-Bostwick C, Mutlu GM, Budinger GR, Chandel NS. 
In:
J Biol Chem (2013) 288(2): 770-7 
Gene expression profiling following NRF2 and KEAP1 siRNA knockdown in human lung fibroblasts identifies CCL11/Eotaxin-1 as a novel NRF2 regulated gene.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fourtounis J1, Wang IM, Mathieu MC, Claveau D, Loo T, Jackson AL, Peters MA, Therien AG, Boie Y, Crackower MA. 
In:
Respir Res (2012) 13: 92 
Effect of ?-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells.
Categories:
Primary Cells and Media 
Authors:
Kuzumaki N1, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Nagase H, Okada Y, Okano HJ, Yamashita JK, Okano H, Suzuki T, Narita M. 
In:
Br J Cancer (2012) 106(6): 1148-52 
Combination of roflumilast with a beta-2 adrenergic receptor agonist inhibits proinflammatory and profibrotic mediator release from human lung fibroblasts
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tannheimer SL1, Wright CD, Salmon M 
In:
Respir Res (2012) 13: 28 
Inhibition of histone lysine methylation enhances cancer-testis antigen expression in lung cancer cells: implications for adoptive immunotherapy of cancer.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rao M1, Chinnasamy N, Hong JA, Zhang Y, Zhang M, Xi S, Liu F, Marquez VE, Morgan RA, Schrump DS. 
In:
Cancer Res (2011) 71(12): 4192-204 
Mapping and analysis of chromatin state dynamics in nine human cell types.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE 
In:
Nature (2011) 473(7345): 43-9 
Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Circadian disruption accelerates tumor growth and angio/stromagenesis through a Wnt signaling pathway.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yasuniwa Y, Izumi H, Wang KY, Shimajiri S, Sasaguri Y, Kawai K, Kasai H, Shimada T, Miyake K, Kashiwagi E, Hirano G, Kidani A, Akiyama M, Han B, Wu Y, Ieiri I, Higuchi S, Kohno K. 
In:
PLoS ONE (2010) 5(12): e15330 
Phosphoproteomics profiling of human skin fibroblast cells reveals pathways and proteins affected by low doses of ionizing radiation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang F, Waters KM, Miller JH, Gritsenko MA, Zhao R, Du X, Livesay EA, Purvine SO, Monroe ME, Wang Y, Camp DG 2nd, Smith RD, Stenoien DL. 
In:
PLoS ONE (2010) 5(11): e14152 
Adipose tissue progenitor cells directly interact with endothelial cells to induce vascular network formation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Cell-permeable peptide DEPDC1-ZNF224 interferes with transcriptional repression and oncogenicity in bladder cancer cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T. 
In:
Cancer Res (2010) 70(14): 5829-39 
Cell contraction forces in scaffolds with varying pore size and cell density.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Corin KA, Gibson LJ. 
In:
Biomaterials (2010) 31(18): 4835-45 
Estrogen receptor beta is a novel therapeutic target for photoaging.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chang KC1, Wang Y, Oh IG, Jenkins S, Freedman LP, Thompson CC, Chung JH, Nagpal S. 
In:
Mol Pharmacol (2010) 77(5): 744-50 
The extracellular matrix glycoprotein elastin microfibril interface located protein 2: a dual role in the tumor microenvironment.
Categories:
Primary Cells and Media 
Authors:
Mongiat M, Marastoni S, Ligresti G, Lorenzon E, Schiappacassi M, Perris R, Frustaci S, Colombatti A. 
In:
Neoplasia (2010) 12(4): 294-304 
Phosphatase inhibitors with anti-angiogenic effect in vitro.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sylvest L1, Bendiksen CD, Houen G. 
In:
APMIS (2010) 118(1): 49-59 
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao W1, Bover L, Cho M, Wen X, Hanabuchi S, Bao M, Rosen DB, Wang YH, Shaw JL, Du Q, Li C, Arai N, Yao Z, Lanier LL, Liu YJ. 
In:
J Exp Med (2009) 206(7): 1603-14 
alpha,beta-Unsaturated aldehydes contained in cigarette smoke elicit IL-8 release in pulmonary cells through mitogen-activated protein kinases.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moretto N1, Facchinetti F, Southworth T, Civelli M, Singh D, Patacchini R. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 296(5): L839-48 
Tumor and vascular targeting of a novel oncolytic measles virus retargeted against the urokinase receptor.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jing Y1, Tong C, Zhang J, Nakamura T, Iankov I, Russell SJ, Merchan JR. 
In:
Cancer Res (2009) 69(4): 1459-68 
IL-13 induces a bronchial epithelial phenotype that is profibrotic
Categories:
Primary Cells and Media 
Authors:
Malavia NK, Mih JD, Raub CB, Dinh BT, George SC 
In:
Respir Res (2008) 09: 27 
Epithelial-derived TGF-beta2 modulates basal and wound-healing subepithelial matrix homeostasis.
Categories:
Primary Cells and Media 
Authors:
Thompson HG, Mih JD, Krasieva TB, Tromberg BJ, George SC. 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 291(6): 1277-85 
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