Fibroblast, dermal (NHDF-Neo), human neonatal

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Primary human neonatal dermal fibroblasts derived from neonatal foreskins.

Cell Type:
Fibroblast
Tissue Origin:
dermal
Species:
human
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Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Fibroblast Growth Medium-2

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P2

DT-130

 1e5 95-97% 79-97% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2

DT-130

 5e5 96-98% 83-97% Plasmid (general) 2 µg 100 µl 4D X-Unit
NHDF

U-020

 5e5 85-95% 85-90% Plasmid (general) 5 µg 100 µl I/II/2b
P2 96-DT-130 1e5 97.4-99.4% 80-92% Plasmid (general) 0.4 µg 20 µl 96-well
AD2

CV-142 or DC-142

 5e4 40-60% 40-75% Plasmid (general) 16 µg 350 µl 4D Y-Unit

Citations (51)

Regeneration of diaphragm with bio-3D cellular patch
Categories:
Primary Cells and Media 
Authors:
Zhang XY1, Yanagi Y2, Sheng Z3, Nagata K2, Nakayama K4, Taguchi T2 
In:
Biomaterials (2018) 167: 1-14 
Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells
Categories:
Transfection 
Authors:
Marta Trevisan, Giovanna Desole, Giulia Costanzi, Enrico Lavezzo, Giorgio Palù, and Luisa Barzon* 
In:
Int J Mol Sci (2017) 18(1): 206 
Integrative transcriptome network analysis of iPSC-derived neurons from schizophrenia and schizoaffective disorder patients with 22q11.2 deletion.
Categories:
Transfection 
Authors:
Lin M, Pedrosa E, Hrabovsky A, Chen J, Puliafito BR, Gilbert SR, Zheng D, Lachman HM. 
In:
BMC Biol. (2016) 10(1): 105 
Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology
Categories:
Transfection 
Authors:
Ying Sun, Jane Florer, Christopher N. Mayhew, Zhanfeng Jia, Zhiying Zhao, Kui Xu, Huimin Ran, Benjamin Liou, Wujuan Zhang, Kenneth D. R. Setchell, Jianguo Gu, Gregory A. Grabowski 
In:
PLoS ONE (2015) 10(3): e0118771 
Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10\'s inhibitory mechanisms.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
Categories:
Transfection 
Authors:
Lin S, Staahl BT, Alla RK, Doudna JA. 
In:
eLife (2014) 3: e04766 
Cell surface profiling using high-throughput flow cytometry: a platform for biomarker discovery and analysis of cellular heterogeneity.
Categories:
Primary Cells and Media, Bioassay  
Authors:
Gedye CA, Hussain A, Paterson J, Smrke A, Saini H, Sirskyj D, Pereira K, Lobo N, Stewart J, Go C, Ho J, Medrano M, Hyatt E, Yuan J, Lauriault S, Kondratyev M, van den Beucken T, Jewett M, Dirks P, Guidos CJ, Danska J, Wang J, Wouters B, Neel B, Rottapel R, Ailles LE 
In:
PLoS ONE (2014) 9(8): ePub 
Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden's nodes in osteoarthritis at clinical presentation
Categories:
Primary Cells and Media 
Authors:
Baboolal TG, Boxall SA, Churchman SM, Buckley CT, Jones E, McGonagle D. 
In:
Arthritis Res Ther (2014) 16(3): R119 
Derivation of human differential photoreceptor cells from adult human dermal fibroblasts by defined combinations of CRX, RAX, OTX2 and NEUROD
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Seko Y1, Azuma N, Ishii T, Komuta Y, Miyamoto K, Miyagawa Y, Kaneda M, Umezawa A. 
In:
Genes Cells (2014) 19(3): 198-208 
Differential expression of tripartite motif-containing family in normal human dermal fibroblasts in response to porcine endogenous retrovirus infection
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimsa MW, Strzalka-Mrozik B, Kimsa MC, Mazurek U, Kruszniewska-Rajs C, Gola J, Adamska J, Twardoch M 
In:
Folia Biol (Praha) (2014) 60(3): 144-51 
Anacardic acid enhances the anticancer activity of liposomal mitoxantrone towards melanoma cell lines - in vitro studies
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Legut M, Lipka D, Filipczak N, Piwoni A, Kozubek A, Gubernator J. 
In:
Int J Nanomedicine (2014) 9: 653-68 
Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pavelin J, Reynolds N, Chiweshe S, Wu G, Tiribassi R, Grey F 
In:
PLoS Pathog (2013) 9(12): e1003820 
Birt-Hogg-Dube syndrome is a novel ciliopathy
Categories:
Primary Cells and Media 
Authors:
Luijten MN1, Basten SG, Claessens T, Vernooij M, Scott CL, Janssen R, Easton JA, Kamps MA, Vreeburg M, Broers JL, van Geel M, Menko FH, Harbottle RP, Nookala RK, Tee AR, Land SC, Giles RH, Coull BJ, van Steensel MA. 
In:
Hum Mol Genet (2013) 22(21: 4383-97 
A small-molecule blocking ribonucleotide reductase holoenzyme formation inhibits cancer cell growth and overcomes drug resistance
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou B, Su L, Hu S, Hu W, Yip ML, Wu J, Gaur S, Smith DL, Yuan YC, Synold TW, Horne D, Yen Y 
In:
Cancer Res (2013) 73(21): 6484-93 
Pleurocidin-family cationic antimicrobial peptides mediate lysis of multiple myeloma cells and impair the growth of multiple myeloma xenografts.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hilchie AL1, Conrad DM, Coombs MR, Zemlak T, Doucette CD, Liwski RS, Hoskin DW. 
In:
Leuk Lymphoma (2013) 54(10): 10 
A bioengineered heterotypic stroma-cancer microenvironment model to study pancreatic ductal adenocarcinoma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drifka CR, Eliceiri KW, Weber SM, Kao WJ 
In:
Lab Chip (2013) 13(19): 3965-75 
Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.
Categories:
Primary Cells and Media 
Authors:
Qin Q, Penkert RR, Kalejta RF 
In:
J Virol (2013) 87(17): 9886-94 
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ricotti L, Fujie T, Vazão H, Ciofani G, Marotta R, Brescia R, Filippeschi C, Corradini I, Matteoli M, Mattoli V, Ferreira L, Menciassi A. 
In:
PLoS ONE (2013) 8(8): e71707 
Human dermal stem/progenitor cell-derived conditioned medium ameliorates ultraviolet a-induced damage of normal human dermal fibroblasts
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shim JH, Park JY, Lee MG, Kang HH, Lee TR, Shin DW 
In:
PLoS ONE (2013) 8(7): e67604 
Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Hellebrin and its aglycone form hellebrigenin display similar in vitro growth inhibitory effects in cancer cells and binding profiles to the alpha subunits of the Na+/K+-ATPase
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moreno Y Banuls L1, Katz A, Miklos W, Cimmino A, Tal DM, Ainbinder E, Zehl M, Urban E, Evidente A, Kopp B, Berger W, Feron O, Karlish S, Kiss R. 
In:
Mol Cancer (2013) 12: 33 
Smaug/SAMD4A restores translational activity of CUGBP1 and suppresses CUG-induced myopathy
Categories:
Primary Cells and Media, Transfection 
Authors:
de Haro M, Al-Ramahi I, Jones KR, Holth JK, Timchenko LT, Botas J. 
In:
PLoS Genet (2013) 9(4): e1003445 
Production of a human neutralizing monoclonal antibody and its crystal structure in complex with ectodomain 3 of the interleukin-13 receptor a1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Redpath NT, Xu Y, Wilson NJ, Fabri LJ, Baca M, Andrews AE, Braley H, Lu P, Ireland C, Ernst RE, Woods A, Forrest G, An Z, Zaller DM, Strohl WR, Luo CS, Czabotar PE, Garrett TP, Hilton DJ, Nash AD, Zhang JG, Nicola NA. 
In:
Biochem J (2013) 451(2): 165-75 
Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane
Categories:
Primary Cells and Media 
Authors:
Janek T, Krasowska A, Radwanska A, Lukaszewicz M. 
In:
PLoS ONE (2013) 8(3): e57991 
Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors.
Categories:
Primary Cells and Media 
Authors:
Islas JF1, Liu Y, Weng KC, Robertson MJ, Zhang S, Prejusa A, Harger J, Tikhomirova D, Chopra M, Iyer D, Mercola M, Oshima RG, Willerson JT, Potaman VN, Schwartz RJ. 
In:
Proc Natl Acad Sci USA (2012) 109(32): 13016-21 
Thiol-ene-based biological/synthetic hybrid biomatrix for 3-D living cell culture.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xu K1, Fu Y, Chung W, Zheng X, Cui Y, Hsu IC, Kao WJ. 
In:
Acta Biomaterialia (2012) 8(7): 2504-16 
Discoidin domain receptors regulate the migration of primary human lung fibroblasts through collagen matrices.
Categories:
Primary Cells and Media 
Authors:
Ruiz PA1, Jarai G. 
In:
Fibrogenesis Tissue Repair (2012) 5: 3 
Proteomic identification of protein targets for 15-deoxy-?(12,14)-prostaglandin J2 in neuronal plasma membrane
Categories:
Primary Cells and Media 
Authors:
Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T 
In:
PLoS ONE (2011) 6(3): e17552 
Phosphoproteomics profiling of human skin fibroblast cells reveals pathways and proteins affected by low doses of ionizing radiation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang F, Waters KM, Miller JH, Gritsenko MA, Zhao R, Du X, Livesay EA, Purvine SO, Monroe ME, Wang Y, Camp DG 2nd, Smith RD, Stenoien DL. 
In:
PLoS ONE (2010) 5(11): e14152 
Cellular and viral control over the initial events of human cytomegalovirus experimental latency in CD34+ cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Saffert RT, Penkert RR, Kalejta RF 
In:
J Virol (2010) 84(11): 5594-604 
Human induced pluripotent stem cells free of vector and transgene sequences.
Categories:
Transfection 
Authors:
Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. 
In:
Science (2009) 324(5928): 797-801 
Tumor and vascular targeting of a novel oncolytic measles virus retargeted against the urokinase receptor.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jing Y1, Tong C, Zhang J, Nakamura T, Iankov I, Russell SJ, Merchan JR. 
In:
Cancer Res (2009) 69(4): 1459-68 
Comparison of zinc finger nucleases for use in gene targeting in mammalian cells
Categories:
Transfection 
Authors:
Pruett-Miller SM, Connelly JP, Maeder ML, Joung JK, Porteus MH 
In:
Mol Ther (2008) 16(4): 707-17 
Human collagen-Krox (hc-Krox) up-regulates type I collagen expression in normal and scleroderma fibroblasts through interaction with Sp1 and Sp3 transcription factors
Categories:
Transfection 
Authors:
Kypriotou M, Beauchef G, Chadjichristos C, Widom R, Renard E, Jimenez S, Korn J, Maquart FX, Oddos T, Von Stetten O, Pujol JP, Galéra P 
In:
J Biol Chem (2007) 282(44): 32000-14 
The autoregulatory and transactivating functions of the human cytomegalovirus IE86 protein use independent mechanisms for promoter binding
Categories:
Transfection 
Authors:
Petrik DT, Schmitt KP, Stinski MF 
In:
J Virol (2007) 81(11): 5807-18 
A lethal defect of mitochondrial and peroxisomal fission
Categories:
Transfection 
Authors:
Waterham HR, Koster J, van Roermund CW, Mooyer PA, Wanders RJ, Leonard JV 
In:
N Engl J Med (2007) 356(17): 1736-41 
The p16INK4a tumor suppressor controls p21WAF1 induction in response to ultraviolet light
Categories:
Transfection 
Authors:
Al-Mohanna MA, Al-Khalaf HH, Al-Yousef N, Aboussekhra A 
In:
Nucleic Acids Res (2007) 35(1): 223-233 
Mucolipin-1 Is a Lysosomal Membrane Protein Required for Intracellular Lactosylceramide Traffic
Categories:
Transfection 
Authors:
Pryor PR, Reimann F, Gribble FM, Luzio JP 
In:
Traffic (2006) 7(10): 1388-1398 
Identification of the gene encoding the enzyme deficient in mucopolysaccharidosis IIIC (Sanfilippo disease type C)
Categories:
Transfection 
Authors:
Fan X, Zhang H, Zhang S, Bagshaw RD, Tropak MB, Callahan JW, Mahuran DJ 
In:
Am J Hum Genet (2006) 79(4): 738-44 
Biphasic regulation by bile acids of dermal fibroblast proliferation through regulation of cAMP production and COX-2 expression level
Categories:
Transfection 
Authors:
Meng J, Ceryak S, Arastu Z, Jones L, Epstein L, Bouscarel B 
In:
Am J Physiol Cell Physiol (2006) 291(3): C546-54 
Increased levels of transforming growth factor beta receptor type I and up-regulation of matrix gene program: A model of scleroderma
Categories:
Transfection 
Authors:
Pannu J, Gardner H, Shearstone JR, Smith E, Trojanowska M 
In:
Arthritis Rheum (2006) 54(9): 3011-21 
Bile acids stimulate PKCautophosphorylation and activation; role in the attenuation of prostaglandin E1-induced cAMP production in human dermal fibroblasts
Categories:
Transfection 
Authors:
Le M, Krilov L, Meng J, Chapin-Kennedy K, Ceryak S, Bouscarel B 
In:
Am J Physiol Gastrointest Liver Physiol (2006) 291(2): G275-87 
Apoptin Nucleocytoplasmic Shuttling Is Required for Cell Type-Specific Localization, Apoptosis, and Recruitment of the Anaphase-Promoting Complex/Cyclosome to PML Bodies
Categories:
Transfection 
Authors:
Heilman DW, Teodoro JG, Green MR 
In:
J Virol (2006) 80(15): 7535-45 
Cellular p32 recruits cytomegalovirus kinase pUL97 to redistribute the nuclear lamina
Categories:
Transfection 
Authors:
Marschall M, Marzi A, Aus dem Siepen P, Jochmann R, Kalmer M, Auerochs S, Lischka P, Leis M and Stamminger T 
In:
J Biol Chem (2005) 280(39): 33357-33367 
The human solute carrier gene SLC35B4 encodes a bifunctional nucleotide sugar transporter with specificity for UDP-xylose and UDP-N-acetylglucosamine
Categories:
Transfection 
Authors:
Ashikov A, Routier F, Fuhlrott J, Helmus Y, Wild MK, Gerardy-Schahn R and Bakker H 
In:
J Biol Chem (2005) 280(29): 27230-27235 
Lowe syndrome protein Ocrl1 is translocated to membrane ruffles upon Rac GTPase activation : a new perspective on Lowe syndrome pathophysiology
Categories:
Transfection 
Authors:
Faucherre A, Desbois P, Nagano F, Satre V, Lunardi J, Gacon G and Dorseuil O 
In:
Hum Mol Genet (2005) 14(11): 1441-1448 
A lack of Birbeck granules in Langerhans cells is associated with a naturally occurring point mutation in the human Langerin gene
Categories:
Transfection 
Authors:
Verdijk P, Dijkman R, Plasmeijer EI, Mulder AA, Zoutman WH, Mieke Mommaas A, Tensen CP 
In:
J Invest Dermatol (2005) 124(4): 714-717 
The steroid receptor co-activator-1 (SRC-1) potentiates TGF-beta/Smad signaling: role of p300/CBP
Categories:
Transfection 
Authors:
Dennler S, Pendaries V, Tacheau C, Costas MA, Mauviel A and Verrecchia F 
In:
Oncogene (2005) 24(11): 1936-1945 
DNA end joining becomes less efficient and more error-prone during cellular senescence
Categories:
Transfection 
Authors:
Seluanov A, Mittelman D, Pereira-Smith OM, Wilson JH and Gorbunova V 
In:
Proc Natl Acad Sci USA (2004) 101(20): 7624-7629 
Disruption of basal JNK activity differentially affects key fibroblast function important for wound healing
Categories:
Transfection 
Authors:
Javelaud D, Laboureau J, Gabison E, Verrecchia F and Mauviel A 
In:
J Biol Chem (2003) 278(27): 24624-24628 
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