Fibroblast, dermal (NHDF-Neo), human neonatal

Primary human neonatal dermal fibroblasts derived from neonatal foreskins.

Cell Type:
Fibroblast
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM is a defined medium system and does not contain serum

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P2

DT-130

1e5 95-97% 79-97% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2

DT-130

5e5 96-98% 83-97% Plasmid (general) 2 µg 100 µl 4D X-Unit
NHDF

U-020

5e5 85-95% 85-90% Plasmid (general) 5 µg 100 µl I/II/2b
P2 96-DT-130 1e5 97.4-99.4% 80-92% Plasmid (general) 0.4 µg 20 µl Shuttle
AD2

CV-142 or DC-142

5e4 40-60% 40-75% Plasmid (general) 16 µg 350 µl 4D Y-Unit

Citations (50)

Categories:
Transfection 
Authors:
Marta Trevisan, Giovanna Desole, Giulia Costanzi, Enrico Lavezzo, Giorgio Palù, and Luisa Barzon* 
In:
Int J Mol Sci (2017) 18(1): 206 
Categories:
Transfection 
Authors:
Lin M, Pedrosa E, Hrabovsky A, Chen J, Puliafito BR, Gilbert SR, Zheng D, Lachman HM. 
In:
BMC Biol. (2016) 10(1): 105 
Categories:
Transfection 
Authors:
Ying Sun, Jane Florer, Christopher N. Mayhew, Zhanfeng Jia, Zhiying Zhao, Kui Xu, Huimin Ran, Benjamin Liou, Wujuan Zhang, Kenneth D. R. Setchell, Jianguo Gu, Gregory A. Grabowski 
In:
PLoS ONE (2015) 10(3): e0118771 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Categories:
Transfection 
Authors:
Lin S, Staahl BT, Alla RK, Doudna JA. 
In:
eLife (2014) 3: e04766 
Categories:
Primary Cells and Media, Bioassay  
Authors:
Gedye CA, Hussain A, Paterson J, Smrke A, Saini H, Sirskyj D, Pereira K, Lobo N, Stewart J, Go C, Ho J, Medrano M, Hyatt E, Yuan J, Lauriault S, Kondratyev M, van den Beucken T, Jewett M, Dirks P, Guidos CJ, Danska J, Wang J, Wouters B, Neel B, Rottapel R, Ailles LE 
In:
PLoS ONE (2014) 9(8): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Baboolal TG, Boxall SA, Churchman SM, Buckley CT, Jones E, McGonagle D. 
In:
Arthritis Res Ther (2014) 16(3): R119 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Seko Y1, Azuma N, Ishii T, Komuta Y, Miyamoto K, Miyagawa Y, Kaneda M, Umezawa A. 
In:
Genes Cells (2014) 19(3): 198-208 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimsa MW, Strzalka-Mrozik B, Kimsa MC, Mazurek U, Kruszniewska-Rajs C, Gola J, Adamska J, Twardoch M 
In:
Folia Biol (Praha) (2014) 60(3): 144-51 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Legut M, Lipka D, Filipczak N, Piwoni A, Kozubek A, Gubernator J. 
In:
Int J Nanomedicine (2014) 9: 653-68 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pavelin J, Reynolds N, Chiweshe S, Wu G, Tiribassi R, Grey F 
In:
PLoS Pathog (2013) 9(12): e1003820 
Categories:
Primary Cells and Media 
Authors:
Luijten MN1, Basten SG, Claessens T, Vernooij M, Scott CL, Janssen R, Easton JA, Kamps MA, Vreeburg M, Broers JL, van Geel M, Menko FH, Harbottle RP, Nookala RK, Tee AR, Land SC, Giles RH, Coull BJ, van Steensel MA. 
In:
Hum Mol Genet (2013) 22(21: 4383-97 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou B, Su L, Hu S, Hu W, Yip ML, Wu J, Gaur S, Smith DL, Yuan YC, Synold TW, Horne D, Yen Y 
In:
Cancer Res (2013) 73(21): 6484-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hilchie AL1, Conrad DM, Coombs MR, Zemlak T, Doucette CD, Liwski RS, Hoskin DW. 
In:
Leuk Lymphoma (2013) 54(10): 10 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drifka CR, Eliceiri KW, Weber SM, Kao WJ 
In:
Lab Chip (2013) 13(19): 3965-75 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Categories:
Primary Cells and Media 
Authors:
Qin Q, Penkert RR, Kalejta RF 
In:
J Virol (2013) 87(17): 9886-94 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ricotti L, Fujie T, Vazão H, Ciofani G, Marotta R, Brescia R, Filippeschi C, Corradini I, Matteoli M, Mattoli V, Ferreira L, Menciassi A. 
In:
PLoS ONE (2013) 8(8): e71707 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shim JH, Park JY, Lee MG, Kang HH, Lee TR, Shin DW 
In:
PLoS ONE (2013) 8(7): e67604 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moreno Y Banuls L1, Katz A, Miklos W, Cimmino A, Tal DM, Ainbinder E, Zehl M, Urban E, Evidente A, Kopp B, Berger W, Feron O, Karlish S, Kiss R. 
In:
Mol Cancer (2013) 12: 33 
Categories:
Primary Cells and Media, Transfection 
Authors:
de Haro M, Al-Ramahi I, Jones KR, Holth JK, Timchenko LT, Botas J. 
In:
PLoS Genet (2013) 9(4): e1003445 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Redpath NT, Xu Y, Wilson NJ, Fabri LJ, Baca M, Andrews AE, Braley H, Lu P, Ireland C, Ernst RE, Woods A, Forrest G, An Z, Zaller DM, Strohl WR, Luo CS, Czabotar PE, Garrett TP, Hilton DJ, Nash AD, Zhang JG, Nicola NA. 
In:
Biochem J (2013) 451(2): 165-75 
Categories:
Primary Cells and Media 
Authors:
Janek T, Krasowska A, Radwanska A, Lukaszewicz M. 
In:
PLoS ONE (2013) 8(3): e57991 
Categories:
Primary Cells and Media 
Authors:
Islas JF1, Liu Y, Weng KC, Robertson MJ, Zhang S, Prejusa A, Harger J, Tikhomirova D, Chopra M, Iyer D, Mercola M, Oshima RG, Willerson JT, Potaman VN, Schwartz RJ. 
In:
Proc Natl Acad Sci USA (2012) 109(32): 13016-21 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xu K1, Fu Y, Chung W, Zheng X, Cui Y, Hsu IC, Kao WJ. 
In:
Acta Biomaterialia (2012) 8(7): 2504-16 
Categories:
Primary Cells and Media 
Authors:
Ruiz PA1, Jarai G. 
In:
Fibrogenesis Tissue Repair (2012) 5: 3 
Categories:
Primary Cells and Media 
Authors:
Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T 
In:
PLoS ONE (2011) 6(3): e17552 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang F, Waters KM, Miller JH, Gritsenko MA, Zhao R, Du X, Livesay EA, Purvine SO, Monroe ME, Wang Y, Camp DG 2nd, Smith RD, Stenoien DL. 
In:
PLoS ONE (2010) 5(11): e14152 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Saffert RT, Penkert RR, Kalejta RF 
In:
J Virol (2010) 84(11): 5594-604 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jing Y1, Tong C, Zhang J, Nakamura T, Iankov I, Russell SJ, Merchan JR. 
In:
Cancer Res (2009) 69(4): 1459-68 
Categories:
Transfection 
Authors:
Pruett-Miller SM, Connelly JP, Maeder ML, Joung JK, Porteus MH 
In:
Mol Ther (2008) 16(4): 707-17 
Categories:
Transfection 
Authors:
Kypriotou M, Beauchef G, Chadjichristos C, Widom R, Renard E, Jimenez S, Korn J, Maquart FX, Oddos T, Von Stetten O, Pujol JP, Galéra P 
In:
J Biol Chem (2007) 282(44): 32000-14 
Categories:
Transfection 
Authors:
Petrik DT, Schmitt KP, Stinski MF 
In:
J Virol (2007) 81(11): 5807-18 
Categories:
Transfection 
Authors:
Waterham HR, Koster J, van Roermund CW, Mooyer PA, Wanders RJ, Leonard JV 
In:
N Engl J Med (2007) 356(17): 1736-41 
Categories:
Transfection 
Authors:
Al-Mohanna MA, Al-Khalaf HH, Al-Yousef N, Aboussekhra A 
In:
Nucleic Acids Res (2007) 35(1): 223-233 
Categories:
Transfection 
Authors:
Pryor PR, Reimann F, Gribble FM, Luzio JP 
In:
Traffic (2006) 7(10): 1388-1398 
Categories:
Transfection 
Authors:
Fan X, Zhang H, Zhang S, Bagshaw RD, Tropak MB, Callahan JW, Mahuran DJ 
In:
Am J Hum Genet (2006) 79(4): 738-44 
Categories:
Transfection 
Authors:
Meng J, Ceryak S, Arastu Z, Jones L, Epstein L, Bouscarel B 
In:
Am J Physiol Cell Physiol (2006) 291(3): C546-54 
Categories:
Transfection 
Authors:
Pannu J, Gardner H, Shearstone JR, Smith E, Trojanowska M 
In:
Arthritis Rheum (2006) 54(9): 3011-21 
Categories:
Transfection 
Authors:
Le M, Krilov L, Meng J, Chapin-Kennedy K, Ceryak S, Bouscarel B 
In:
Am J Physiol Gastrointest Liver Physiol (2006) 291(2): G275-87 
Categories:
Transfection 
Authors:
Marschall M, Marzi A, Aus dem Siepen P, Jochmann R, Kalmer M, Auerochs S, Lischka P, Leis M and Stamminger T 
In:
J Biol Chem (2005) 280(39): 33357-33367 
Categories:
Transfection 
Authors:
Ashikov A, Routier F, Fuhlrott J, Helmus Y, Wild MK, Gerardy-Schahn R and Bakker H 
In:
J Biol Chem (2005) 280(29): 27230-27235 
Categories:
Transfection 
Authors:
Faucherre A, Desbois P, Nagano F, Satre V, Lunardi J, Gacon G and Dorseuil O 
In:
Hum Mol Genet (2005) 14(11): 1441-1448 
Categories:
Transfection 
Authors:
Verdijk P, Dijkman R, Plasmeijer EI, Mulder AA, Zoutman WH, Mieke Mommaas A, Tensen CP 
In:
J Invest Dermatol (2005) 124(4): 714-717 
Categories:
Transfection 
Authors:
Dennler S, Pendaries V, Tacheau C, Costas MA, Mauviel A and Verrecchia F 
In:
Oncogene (2005) 24(11): 1936-1945 
Categories:
Transfection 
Authors:
Seluanov A, Mittelman D, Pereira-Smith OM, Wilson JH and Gorbunova V 
In:
Proc Natl Acad Sci USA (2004) 101(20): 7624-7629 
Categories:
Transfection 
Authors:
Javelaud D, Laboureau J, Gabison E, Verrecchia F and Mauviel A 
In:
J Biol Chem (2003) 278(27): 24624-24628 
Categories:
Primary Cells and Media 
Authors:
Zhang XY1, Yanagi Y2, Sheng Z3, Nagata K2, Nakayama K4, Taguchi T2 
In:
Biomaterials () : 1-14 
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