Generation of iPSC lines from control and GD2 fibroblasts: Fibroblasts were nucleofected (program U-20) with episomal plasmids pCLXE-hOct3/4-shp53, pCLXE- hSox2-Klf4, pCLXE- hLmyc-Lin28, and pCLXE-GFP. Six days post-nucleofection, fibroblasts were replated in MEF media in a gelatin-coated 10 cm dish containing 1.07E+6 irradiated mouse embryonic fibroblasts (MEFs). Starting on day 7 post-transfection, cells were fed daily with DMEM/F12 media supplemented with 20% knockout serum replacement, 1 mM L-glutamine, 0.1 mM ß-mercaptoethanol, 0.1 mM nonessential amino acids, and 4 ng/mL basic FGF. Approximately 2 weeks later, discrete colonies with hESC-like morphology were manually excised and replated in mTeSR1 media in tissue culture dishes coated with hESC-qualified matrigel. Lonza summary: This article shows a great example of generating cellular diseased models to study a genetic neuropathy. The Nucleofector device was used to reprogram patient and normal donor fibroblast into iPSC, which were in turn differentiated into neurons. This is the first study that reports abnormal electrophysiological properties in Gaucher Disease type 2 iPSC-derived neurons.