Embryonic Stem Cell (ES), human

Pluripotent stem cells derived from human blastocyst

Cell Type:
Embryonic SC
Tissue Origin:
embryo
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Dermatology/Tissue Engineering
Gastroenterology
Immunotherapy / Hematology
Neurobiology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl Shuttle


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
HSC 1

A-012

1e6 22% 96% Plasmid (general) 2 µg 100 µl I/II/2b
HSC 1

B-016

2e6 78% 57% Plasmid (general) 2 µg 100 µl I/II/2b
HSC 2 A-023 2e6 50% good Plasmid (general) 2 ug 100 µl I/II/2b
HSC 2 A-023 2e6 55% 75% Plasmid (general) 2 ug 100 µl I/II/2b
HSC 2 A-023 1e6 20-30% good Plasmid (general) 2 ug 100 µl I/II/2b


50-60% 50-75% Plasmid (general) 2 µg 100 µl I/II/2b
P3 96-CB-150 2e5 64% ±3 98% ±11 Plasmid (general) 0.8 µg 20 µl Shuttle
P3

CB-150

61-67% 87-98% Plasmid (general) 0.8 µg 20 µl 4D X-Unit

Citations (36)

Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
Categories:
Primary Cells and Media, Transfection 
Authors:
Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. 
In:
eLife (2018) 11;7: 1-29 
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Categories:
Transfection 
Authors:
Koehler KR, Nie J1, Longworth-Mills E, Liu XP, Lee J, Holt JR, Hashino E. 
In:
Nat Biotechnol (2017) 35(6): 583-589 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O 
In:
Nat Protocols (2016) 11(10): 1833-50 
Categories:
Transfection 
Authors:
Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J. 
In:
PLoS ONE (2015) : e0143148 
Categories:
 
Authors:
Jia Liu, Thomas Gaj, Yifeng Yang, Nan Wang, Sailan Shui, Sojung Kim, Chidananda Nagamangala Kanchiswamy, Jin-Soo Kim, Carlos F Barbas III 
In:
Nat Protocols (2015) 10(11): 1842-59 
Categories:
Transfection 
Authors:
SM Byrne, GM Church 
In:
(2015) 35:5A.8: -1-5A.8.22 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morizane R, Lam AQ 
In:
Biomark Insights (2015) 10: 147-52 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
JUDI A. SGAMBATO, TEA SOON PARK, DIANA MILLER, LEELAMMA M. PANICKER, ELLEN SIDRANSKY, YU LUN, OLA AWAD, SØREN M. BENTZEN, ELIAS T. ZAMBIDIS, RICARDO A. FELDMAN 
In:
STEM CELLS TRANSLATIONALMEDICINE (2015) 4(8): 878-86 
Categories:
Transfection 
Authors:
Ying Sun, Jane Florer, Christopher N. Mayhew, Zhanfeng Jia, Zhiying Zhao, Kui Xu, Huimin Ran, Benjamin Liou, Wujuan Zhang, Kenneth D. R. Setchell, Jianguo Gu, Gregory A. Grabowski 
In:
PLoS ONE (2015) 10(3): e0118771 
Categories:
Transfection 
Authors:
Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, Rimchala T, Batt G, Weiss R. 
In:
Nucleic Acids Res (2014) 42(21): 13440-51 
Categories:
 
Authors:
Adam C. Wilkinson, Viviane K. S. Kawata, Judith Schütte, Xuefei Gao, Stella Antoniou, Claudia Baumann, Steven Woodhouse, Rebecca Hannah, Yosuke Tanaka, Gemma Swiers, Victoria Moignard, Jasmin Fisher, Shimauchi Hidetoshi, Marloes R. Tijssen, Marella F. T. R. de Bruijn, Pentao Liu, Berthold Göttgens 
In:
Development (2014) 141(20): 4018-30 
Categories:
Transfection 
Authors:
Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao 
In:
Nat Protocols (2014) 9(10): 2411-24 
Categories:
Transfection 
Authors:
Kim S, Kim D, Cho SW, Kim J, Kim JS 
In:
Genome Res (2014) 24(6): 1012-9 
Categories:
Transfection 
Authors:
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP. 
In:
Proc Natl Acad Sci USA (2014) 42(5): e34 
Categories:
Transfection 
Authors:
Susan M. Byrne, Prashant Mali, and George M. Church 
In:
Methods Enzymol (2014) 546: 119-37 
Categories:
Transfection, Electrophoreses and Analysis  
Authors:
Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F 
In:
Cell (2013) 15(6): 1380-1389 
Categories:
Transfection 
Authors:
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F 
In:
Nat Protocols (2013) 8(11): 2281–2308 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Categories:
Transfection 
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E. 
In:
Genome Res (2013) 23(7): 1182-93 
Categories:
Transfection 
Authors:
Feng Cao,1,2,3 Xiaoyan Xie,1 Timothy Gollan,1 Li Zhao,3 Kazim Narsinh,2 Randall J. Lee,4 and Joseph C. Wu1,2 
In:
Mol Imaging Biol (2010) 12(1): 15-24 
Categories:
Transfection 
Authors:
Tian X1, Hexum MK, Penchev VR, Taylor RJ, Shultz LD, Kaufman DS. 
In:
Stem Cells (2009) 27(11): 2675(85) 
Categories:
Transfection 
Authors:
Stefanovic S, Abboud N, Désilets S, Nury D, Cowan C, Pucéat M. 
In:
J Cell Biol (2009) 186(5): 665-7 
Categories:
Transfection 
Authors:
Placantonakis DG, Tomishima MJ, Lafaille F, Desbordes SC, Jia F, Socci ND, Viale A, Lee H, Harrison N, Tabar V, Studer L. 
In:
Stem Cells (2009) 27: 521-532 
Categories:
Transfection 
Authors:
Fong H, Hohenstein KA, Donovan PJ 
In:
Stem Cells (2008) 26(8): 1931-8 
Categories:
Transfection 
Authors:
Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ 
In:
Stem Cells (2008) 26(6): 1436-43 
Categories:
Transfection 
Authors:
Wilber A, Linehan JL, Tian X, Woll PS, Morris JK, Belur LR, McIvor RS, Kaufman DS 
In:
Stem Cells (2007) 25(11): 2919-27 
Categories:
Transfection 
Authors:
Garcia-Perez JL, Marchetto MC, Muotri AR, Coufal N, Gage FH, Sue O'shea K, Moran JV 
In:
Hum Mol Genet (2007) 16(13): 1569-77 
Categories:
Transfection 
Authors:
Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD 
In:
Nature (2007) 448(7150): 196-9 
Categories:
Transfection 
Authors:
Wang D, Haviland DL, Burns AR, Zsigmond E, Wetsel RA 
In:
Proc Natl Acad Sci USA (2007) 104(11): 4449-54 
Categories:
Transfection 
Authors:
Cai L, Ye Z, Zhou BY, Mali P, Zhou C, Cheng L 
In:
Cell Res (2007) 17(1): 62-72 
Categories:
Transfection 
Authors:
Koch P, Siemen H, Biegler A, Itskovitz-Eldor J, Brustle O 
In:
Nucleic Acids Res (2006) 34(18): e120 
Categories:
Transfection 
Authors:
Sidhu KS, Lie KH, Tuch BE 
In:
Stem Cells Dev (2006) 15(5): 741-7 
Categories:
Transfection 
Authors:
Siemen H, Nix M, Endl E, Koch P, Itskovitz-Eldor J and Brustle O 
In:
Stem Cells Dev (2005) 14(4): 378-383 
Categories:
Transfection 
Authors:
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM 
In:
Stem Cells (2004) 22(4): 531-543 
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