Embryonic Stem Cell (ES), human

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Pluripotent stem cells derived from human blastocyst

Cell Type:
Embryonic SC
Tissue Origin:
embryo
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Dermatology/Tissue Engineering
Gastroenterology
Immunotherapy / Hematology
Neurobiology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
basic 2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 96-well


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
HSC 1

A-012

 1e6 22% 96% Plasmid (general) 2 µg 100 µl I/II/2b
HSC 1

B-016

 2e6 78% 57% Plasmid (general) 2 µg 100 µl I/II/2b
HSC 2 A-023 2e6 50% good Plasmid (general) 2 ug 100 µl I/II/2b
HSC 2 A-023 2e6 55% 75% Plasmid (general) 2 ug 100 µl I/II/2b
HSC 2 A-023 1e6 20-30% good Plasmid (general) 2 ug 100 µl I/II/2b


50-60% 50-75% Plasmid (general) 2 µg 100 µl I/II/2b
P3 96-CB-150 2e5 64% ±3 98% ±11 Plasmid (general) 0.8 µg 20 µl 96-well
P3

CB-150

 61-67% 87-98% Plasmid (general) 0.8 µg 20 µl 4D X-Unit

Citations (37)

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein.
Categories:
Primary Cells and Media, Transfection 
Authors:
Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. 
In:
eLife (2018) 11;7: 1-29 
CRISPR/Cas9-mediated genome editing in naïve human embryonic stem cells
Categories:
Transfection 
Authors:
Eva Z Jacobs, Sharat Warrier, Pieter-Jan Volders, Eva D'haene, Eva Van Lombergen, Lies Vantomme, Margot Van der Jeught, Björn Heindryckx, Björn Menten, Sarah Vergult 
In:
Scientific Reports (2017) 7(1): 16650 
Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
Categories:
Transfection 
Authors:
Koehler KR, Nie J1, Longworth-Mills E, Liu XP, Lee J, Holt JR, Hashino E. 
In:
Nat Biotechnol (2017) 35(6): 583-589 
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O 
In:
Nat Protocols (2016) 11(10): 1833-50 
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo
Categories:
Transfection 
Authors:
Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J. 
In:
PLoS ONE (2015) : e0143148 
Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.
Categories:
 
Authors:
Jia Liu, Thomas Gaj, Yifeng Yang, Nan Wang, Sailan Shui, Sojung Kim, Chidananda Nagamangala Kanchiswamy, Jin-Soo Kim, Carlos F Barbas III 
In:
Nat Protocols (2015) 10(11): 1842-59 
Current Protocols in Stem Cell Biology: CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells.
Categories:
Transfection 
Authors:
SM Byrne, GM Church 
In:
(2015) 35:5A.8: -1-5A.8.22 
Directed Differentiation of Pluripotent Stem Cells into Kidney
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morizane R, Lam AQ 
In:
Biomark Insights (2015) 10: 147-52 
Gaucher Disease-Induced Pluripotent Stem Cells Display Decreased Erythroid Potential and Aberrant Myelopoiesis.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
JUDI A. SGAMBATO, TEA SOON PARK, DIANA MILLER, LEELAMMA M. PANICKER, ELLEN SIDRANSKY, YU LUN, OLA AWAD, SØREN M. BENTZEN, ELIAS T. ZAMBIDIS, RICARDO A. FELDMAN 
In:
STEM CELLS TRANSLATIONALMEDICINE (2015) 4(8): 878-86 
Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology
Categories:
Transfection 
Authors:
Ying Sun, Jane Florer, Christopher N. Mayhew, Zhanfeng Jia, Zhiying Zhao, Kui Xu, Huimin Ran, Benjamin Liou, Wujuan Zhang, Kenneth D. R. Setchell, Jianguo Gu, Gregory A. Grabowski 
In:
PLoS ONE (2015) 10(3): e0118771 
A platform for rapid prototyping of synthetic gene networks in mammalian cells
Categories:
Transfection 
Authors:
Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, Rimchala T, Batt G, Weiss R. 
In:
Nucleic Acids Res (2014) 42(21): 13440-51 
Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification
Categories:
 
Authors:
Adam C. Wilkinson, Viviane K. S. Kawata, Judith Schütte, Xuefei Gao, Stella Antoniou, Claudia Baumann, Steven Woodhouse, Rebecca Hannah, Yosuke Tanaka, Gemma Swiers, Victoria Moignard, Jasmin Fisher, Shimauchi Hidetoshi, Marloes R. Tijssen, Marella F. T. R. de Bruijn, Pentao Liu, Berthold Göttgens 
In:
Development (2014) 141(20): 4018-30 
Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs
Categories:
Transfection 
Authors:
Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao 
In:
Nat Protocols (2014) 9(10): 2411-24 
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
Categories:
Transfection 
Authors:
Kim S, Kim D, Cho SW, Kim J, Kim JS 
In:
Genome Res (2014) 24(6): 1012-9 
DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.
Categories:
Transfection 
Authors:
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP. 
In:
Proc Natl Acad Sci USA (2014) 42(5): e34 
Genome Editing in Human Stem Cells
Categories:
Transfection 
Authors:
Susan M. Byrne, Prashant Mali, and George M. Church 
In:
Methods Enzymol (2014) 546: 119-37 
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Categories:
Transfection, Electrophoreses and Analysis  
Authors:
Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F 
In:
Cell (2013) 15(6): 1380-1389 
Genome engineering using the CRISPR-Cas9 system
Categories:
Transfection 
Authors:
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F 
In:
Nat Protocols (2013) 8(11): 2281–2308 
Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Cancer translocations in human cells induced by zinc finger and TALE nucleases.
Categories:
Transfection 
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E. 
In:
Genome Res (2013) 23(7): 1182-93 
Comparison of Gene-Transfer Efficiency in Human Embryonic Stem Cells
Categories:
Transfection 
Authors:
Feng Cao,1,2,3 Xiaoyan Xie,1 Timothy Gollan,1 Li Zhao,3 Kazim Narsinh,2 Randall J. Lee,4 and Joseph C. Wu1,2 
In:
Mol Imaging Biol (2010) 12(1): 15-24 
Bioluminescent imaging demonstrates that transplanted human embryonic stem cell-derived CD34(+) cells preferentially develop into endothelial cells.
Categories:
Transfection 
Authors:
Tian X1, Hexum MK, Penchev VR, Taylor RJ, Shultz LD, Kaufman DS. 
In:
Stem Cells (2009) 27(11): 2675(85) 
Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate
Categories:
Transfection 
Authors:
Stefanovic S, Abboud N, Désilets S, Nury D, Cowan C, Pucéat M. 
In:
J Cell Biol (2009) 186(5): 665-7 
BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage.
Categories:
Transfection 
Authors:
Placantonakis DG, Tomishima MJ, Lafaille F, Desbordes SC, Jia F, Socci ND, Viale A, Lee H, Harrison N, Tabar V, Studer L. 
In:
Stem Cells (2009) 27: 521-532 
Regulation of Self-renewal and Pluripotency by Sox2 in Human Embryonic Stem Cells
Categories:
Transfection 
Authors:
Fong H, Hohenstein KA, Donovan PJ 
In:
Stem Cells (2008) 26(8): 1931-8 
Nucleofection Mediates High-efficiency Stable Gene Knockdown and Transgene Expression in Human Embryonic Stem Cells
Categories:
Transfection 
Authors:
Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ 
In:
Stem Cells (2008) 26(6): 1436-43 
Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer
Categories:
Transfection 
Authors:
Wilber A, Linehan JL, Tian X, Woll PS, Morris JK, Belur LR, McIvor RS, Kaufman DS 
In:
Stem Cells (2007) 25(11): 2919-27 
LINE-1 retrotransposition in human embryonic stem cells
Categories:
Transfection 
Authors:
Garcia-Perez JL, Marchetto MC, Muotri AR, Coufal N, Gage FH, Sue O'shea K, Moran JV 
In:
Hum Mol Genet (2007) 16(13): 1569-77 
New cell lines from mouse epiblast share defining features with human embryonic stem cells
Categories:
Transfection 
Authors:
Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD 
In:
Nature (2007) 448(7150): 196-9 
A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells
Categories:
Transfection 
Authors:
Wang D, Haviland DL, Burns AR, Zsigmond E, Wetsel RA 
In:
Proc Natl Acad Sci USA (2007) 104(11): 4449-54 
Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions
Categories:
Transfection 
Authors:
Cai L, Ye Z, Zhou BY, Mali P, Zhou C, Cheng L 
In:
Cell Res (2007) 17(1): 62-72 
Transduction of human embryonic stem cells by ecotropic retroviral vectors
Categories:
Transfection 
Authors:
Koch P, Siemen H, Biegler A, Itskovitz-Eldor J, Brustle O 
In:
Nucleic Acids Res (2006) 34(18): e120 
Transgenic human fetal fibroblasts as feeder layer for human embryonic stem cell lineage selection
Categories:
Transfection 
Authors:
Sidhu KS, Lie KH, Tuch BE 
In:
Stem Cells Dev (2006) 15(5): 741-7 
Nucleofection of human embryonic stem cells
Categories:
Transfection 
Authors:
Siemen H, Nix M, Endl E, Koch P, Itskovitz-Eldor J and Brustle O 
In:
Stem Cells Dev (2005) 14(4): 378-383 
Efficient transfection of embryonic and adult stem cells
Categories:
Transfection 
Authors:
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM 
In:
Stem Cells (2004) 22(4): 531-543 
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