Fibroblast, lung, human normal (NHLF)

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Primay fibroblasts isolated from adult lung tissue

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
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Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Fibroblast Growth Medium-2

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Fibroblast, basic U-023 1e6 54% 68% Plasmid (general) 2 µg 100 µl I/II/2b
Fibroblast, basic U-013 1e6 33% 73% Plasmid (general) 2 µg 100 µl I/II/2b


1e5 50-75% 70-80% Plasmid (general) 0.4 µg 20 µl 96-well


1e5 50-75% 70-80% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CA-158 1e5 86% ±1 57% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CA-158 5e5 80% 64% ±1 Plasmid (general) 2 µg 100 µl 4D X-Unit
P3 CA-158 1e5 75% ±1 74% ±4 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CA-158 5e5 74% ±1 74% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (24)

Single-Step, High-Efficiency CRISPR-Cas9 Genome Editing in Primary Human Disease-Derived Fibroblasts.
Categories:
Primary Cells and Media, Transfection 
Authors:
Martufi M, Good RB, Rapiteanu R, Schmidt T, Patili E, Tvermosegaard K, New M, Nanthakumar CB, Betts J, Blanchard AD, Maratou K. 
In:
CRISPR J (2019) 2: 31-40 
3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections.
Categories:
Primary Cells and Media 
Authors:
Marrazzo P, Maccari S, Taddei A, Bevan L, Telford J, Soriani M, Pezzicoli A 
In:
PLoS ONE (2016) 11(4): e0153985 
Pharmacological properties of JTE-052: a novel potent JAK inhibitor that suppresses various inflammatory responses in vitro and in vivo.
Categories:
Primary Cells and Media 
Authors:
Tanimoto A, Ogawa Y, Oki C, Kimoto Y, Nozawa K, Amano W, Noji S, Shiozaki M, Matsuo A, Shinozaki Y, Matsushita M 
In:
Other (2015) 64(1): 41-51 
Induction of heme oxygenase I (HMOX1) by HPP-4382: a novel modulator of Bach1 activity
Categories:
Primary Cells and Media 
Authors:
Attucks OC, Jasmer KJ, Hannink M, Kassis J, Zhong Z, Gupta S, Victory SF, Guzel M, Polisetti DR, Andrews R, Mjalli AM, Kostura MJ 
In:
PLoS ONE (2014) 9(7): e101044 
Arsenic trioxide inhibits transforming growth factor-ß1-induced fibroblast to myofibroblast differentiation in vitro and bleomycin induced lung fibrosis in vivo
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Luo F, Zhuang Y, Sides MD, Sanchez CG, Shan B, White ES, Lasky JA 
In:
Respir Res (2014) 24: 15:51 
A Screen for Short-Range Paracrine Interactions
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Spencer KH, Kim MY, Hughes CC, Hui EE. 
In:
Integr Biol (Camb). (2014) 6(4): 382-7 
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, Nakae S 
In:
PLoS ONE (2014) 9(1): e86106 
Sphingosine 1-phosphate (S1P) receptor agonists mediate pro-fibrotic responses in normal human lung fibroblasts via S1P2 and S1P3 receptors and Smad-independent signaling
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sobel K, Menyhart K, Killer N, Renault B, Bauer Y, Studer R, Steiner B, Bolli MH, Nayler O, Gatfield J. 
In:
J Biol Chem (2013) 288(21): 14839-51 
IL-25 drives remodelling in allergic airways disease induced by house dust mite.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gregory LG1, Jones CP, Walker SA, Sawant D, Gowers KH, Campbell GA, McKenzie AN, Lloyd CM. 
In:
Tissue Eng (2013) 68(1): 82-90 
Increased Expression of RhoA in Epithelium and Smooth Muscle of Obese Mouse Models: Implications for Isoprenoid Control of Airway Smooth Muscle and Fibroblasts
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ross KR1, Darrah RJ, Hodges CA, Lang L, Kelley TJ. 
In:
Journal of Allergy (2013) 2013: 740973 
Exploring the Interactions between Mesenchymal Stem Cells and Endothelial Cells in Engineered Perivascular Niches
Categories:
Primary Cells and Media 
Authors:
Bita Carrion 
In:
Other (2013) 1: 1-159 
Mitochondrial reactive oxygen species regulate transforming growth factor-ß signaling
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jain M, Rivera S, Monclus EA, Synenki L, Zirk A, Eisenbart J, Feghali-Bostwick C, Mutlu GM, Budinger GR, Chandel NS. 
In:
J Biol Chem (2013) 288(2): 770-7 
Gene expression profiling following NRF2 and KEAP1 siRNA knockdown in human lung fibroblasts identifies CCL11/Eotaxin-1 as a novel NRF2 regulated gene.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fourtounis J1, Wang IM, Mathieu MC, Claveau D, Loo T, Jackson AL, Peters MA, Therien AG, Boie Y, Crackower MA. 
In:
Respir Res (2012) 13: 92 
Reduced elastogenesis: a clue to the arteriosclerosis and emphysematous changes in Schimke immuno-osseous dysplasia?
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morimoto M, Yu Z, Stenzel P, Clewing JM, Najafian B, Mayfield C, Hendson G, Weinkauf JG, Gormley AK, Parham DM, Ponniah U, André JL, Asakura Y, Basiratnia M, Bogdanovic R, Bokenkamp A, Bonneau D, Buck A, Charrow J, Cochat P, Cordeiro I, Deschenes G, Fenkçi MS, Frange P, Fründ S, Fryssira H, Guillen-Navarro E, Keller K, Kirmani S, Kobelka C, Lamfers P, Levtchenko E, Lewis DB, Massella L, McLeod DR, Milford DV, Nobili F, Saraiva JM, Semerci CN, Shoemaker L, Stajic N, Stein A, Taha D, Wand D, Zonana J, Lücke T, Boerkoel CF. 
In:
Orphanet Journal of Rare Diseases (2012) 7: 70 
Effect of ?-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells.
Categories:
Primary Cells and Media 
Authors:
Kuzumaki N1, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Nagase H, Okada Y, Okano HJ, Yamashita JK, Okano H, Suzuki T, Narita M. 
In:
Br J Cancer (2012) 106(6): 1148-52 
Combination of roflumilast with a beta-2 adrenergic receptor agonist inhibits proinflammatory and profibrotic mediator release from human lung fibroblasts
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tannheimer SL1, Wright CD, Salmon M 
In:
Respir Res (2012) 13: 28 
Discoidin domain receptors regulate the migration of primary human lung fibroblasts through collagen matrices.
Categories:
Primary Cells and Media 
Authors:
Ruiz PA1, Jarai G. 
In:
Fibrogenesis Tissue Repair (2012) 5: 3 
Mapping and analysis of chromatin state dynamics in nine human cell types.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE 
In:
Nature (2011) 473(7345): 43-9 
IL-33 Mediates Inflammatory Responses in Human Lung Tissue Cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A. 
In:
J Immunol (2010) 185(10): 5743-50 
alpha,beta-Unsaturated aldehydes contained in cigarette smoke elicit IL-8 release in pulmonary cells through mitogen-activated protein kinases.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moretto N1, Facchinetti F, Southworth T, Civelli M, Singh D, Patacchini R. 
In:
Am J Physiol Lung Cell Mol Physiol (2009) 296(5): L839-48 
TGF-beta 1 induces heparan sulfate 6-O-endosulfatase 1 expression in vitro and in vivo
Categories:
Transfection 
Authors:
Yue X, Li X, Nguyen HT, Chin DR, Sullivan DE, Lasky JA 
In:
J Biol Chem (2008) 283(29): 20397-407 
IL-13 induces a bronchial epithelial phenotype that is profibrotic
Categories:
Primary Cells and Media 
Authors:
Malavia NK, Mih JD, Raub CB, Dinh BT, George SC 
In:
Respir Res (2008) 09: 27 
Epithelial-derived TGF-beta2 modulates basal and wound-healing subepithelial matrix homeostasis.
Categories:
Primary Cells and Media 
Authors:
Thompson HG, Mih JD, Krasieva TB, Tromberg BJ, George SC. 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 291(6): 1277-85 
Modulation of collagen and MMP-1 gene expression in fibroblasts by the immunosuppressive drug rapamycin: A direct role as an anti-fibrotic agent?
Categories:
Transfection 
Authors:
Poulalhon N, Farge D, Roos N, Tacheau C, Neuzillet C, Michel L, Mauviel A, Verrecchia F 
In:
J Biol Chem (2006) 281(44): 33045-52 
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