Fibroblast, lung (DHLF), human COPD

Chronic obstructive pulmonary disease, is a progressive disease that makes it hard to breathe. Chronic bronchitis & emphysema are other names/stages of COPD. COPD can cause coughing that produces large amounts of mucus, wheezing, shortness of breath, chest tightness, and other symptoms.

Cell Type:
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:

Recommended Media

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 CA-158 1e5 73% ±1 72% ±11 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CA-158 5e5 72% 77% ±5 Plasmid (general) 2 µg 100 µl 4D X-Unit