Fibroblast, dermal(NHDF-Ad), human adult

Adult Human primary fibroblasts derived form adult skin tissue.

Cell Type:
Fibroblast
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM is a defined medium system and does not contain serum

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
NHDF U-023 5e5 65-73% 47-101% Plasmid (general) 2 µg 100 µl I/II/2b


1e5 80-95% 90-95% Plasmid (general) 0.4 µg 20 µl Shuttle


1e5 80-95% 90-95% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2 DS-150 1e5 95-97% 91-93% Plasmid (general) 0.4 µg 20 µl Shuttle
P2 FF-113 1e5 90-94% 94-108% Plasmid (general) 0.4 µg 20 µl Shuttle
P2 DS-150 1e5 94-96% 90-92% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2 EO-114 1e5 90-94% 95-99% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2 FF-113 1e5 90-92% 95% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (63)

Categories:
Transfection 
Authors:
Thomas Wohlfahrt , Simon Rauber , Steffen Uebe , Markus Luber , Alina Soare , Arif Ekici , Stefanie Weber , Alexandru-Emil Matei , Chih-Wei Chen , Christiane Maier , Emmanuel Karouzakis , Hans P Kiener , Elena Pachera , Clara Dees , Christian Beyer , Christoph Daniel , Kolja Gelse , Andreas E Kremer , Elisabeth Naschberger , Michael Stürzl , Falk Butter , Michael Sticherling , Susetta Finotto , Alexander Kreuter , Mark H Kaplan , Astrid Jüngel, Steffen Gay , Stephen L Nutt , David W Boykin , Gregory M K Poon , Oliver Distler , Georg Schett , Jörg H W Distler , Andreas Ramming  
In:
Nature (2019) 566 (7744), : 344-349 
Categories:
Transfection 
Authors:
Marta Trevisan, Giovanna Desole, Giulia Costanzi, Enrico Lavezzo, Giorgio Palù, and Luisa Barzon* 
In:
Int J Mol Sci (2017) 18(1): 206 
Categories:
Transfection 
Authors:
Lin M, Pedrosa E, Hrabovsky A, Chen J, Puliafito BR, Gilbert SR, Zheng D, Lachman HM. 
In:
BMC Biol. (2016) 10(1): 105 
Categories:
Transfection 
Authors:
Xianming Wanga, b, i, Shen Chenc, Ingo Burtschera, b, Michael Sterra, b, Anja Hieronimusd, e, j, Fausto Machicaof, j, Harald Staigerd, g, j, Hans-Ulrich Häringd, e, j, Gabriele Ledererk, Thomas Meitingerh, k, Heiko Lickerta, b, i, j, , 
In:
Stem Cells Res. (2016) 2: 273–276 
Categories:
Transfection 
Authors:
Manzini S, Viiri LE, Marttila S, Aalto-Setälä K 
In:
Stem Cell Reports (2015) epub: 1-9 
Categories:
Transfection 
Authors:
Zhao D, Lin M, Chen J, Pedrosa E, Hrabovsky A, Fourcade HM, Zheng D, Lachman HM. 
In:
PLoS ONE (2015) 10(7): 1-24 
Categories:
Primary Cells and Media 
Authors:
Salci KR, Lee JH, Laronde S, Dingwall S, Kushwah R, Fiebig-Comyn A, Leber B, Foley R, Dal Cin A, Bhatia M 
In:
Stem Cells (2015) 33(6): 1839-49 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Categories:
Primary Cells and Media, Bioassay  
Authors:
Gedye CA, Hussain A, Paterson J, Smrke A, Saini H, Sirskyj D, Pereira K, Lobo N, Stewart J, Go C, Ho J, Medrano M, Hyatt E, Yuan J, Lauriault S, Kondratyev M, van den Beucken T, Jewett M, Dirks P, Guidos CJ, Danska J, Wang J, Wouters B, Neel B, Rottapel R, Ailles LE 
In:
PLoS ONE (2014) 9(8): ePub 
Categories:
Primary Cells and Media 
Authors:
Hulikova A, Aveyard N, Harris A, Vaughan-Jones R, Swietach P 
In:
J Biol Chem (2014) 24: 1 
Categories:
Transfection 
Authors:
Reichman S, Terray A, Slembrouck A, Nanteau C, Orieux G, Habeler W, Nandrot EF, Sahel JA, Monville C, Goureau O 
In:
Proc Natl Acad Sci USA (2014) 111(23): 8518-23 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Baboolal TG, Boxall SA, Churchman SM, Buckley CT, Jones E, McGonagle D. 
In:
Arthritis Res Ther (2014) 16(3): R119 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Seko Y1, Azuma N, Ishii T, Komuta Y, Miyamoto K, Miyagawa Y, Kaneda M, Umezawa A. 
In:
Genes Cells (2014) 19(3): 198-208 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimsa MW, Strzalka-Mrozik B, Kimsa MC, Mazurek U, Kruszniewska-Rajs C, Gola J, Adamska J, Twardoch M 
In:
Folia Biol (Praha) (2014) 60(3): 144-51 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Legut M, Lipka D, Filipczak N, Piwoni A, Kozubek A, Gubernator J. 
In:
Int J Nanomedicine (2014) 9: 653-68 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pavelin J, Reynolds N, Chiweshe S, Wu G, Tiribassi R, Grey F 
In:
PLoS Pathog (2013) 9(12): e1003820 
Categories:
Primary Cells and Media 
Authors:
Luijten MN1, Basten SG, Claessens T, Vernooij M, Scott CL, Janssen R, Easton JA, Kamps MA, Vreeburg M, Broers JL, van Geel M, Menko FH, Harbottle RP, Nookala RK, Tee AR, Land SC, Giles RH, Coull BJ, van Steensel MA. 
In:
Hum Mol Genet (2013) 22(21: 4383-97 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou B, Su L, Hu S, Hu W, Yip ML, Wu J, Gaur S, Smith DL, Yuan YC, Synold TW, Horne D, Yen Y 
In:
Cancer Res (2013) 73(21): 6484-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Categories:
Transfection 
Authors:
Chapdelaine P, Coulombe Z, Chikh A, Gérard C, Tremblay JP. 
In:
Mol Ther (2013) 2: 1-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shim JH, Park JY, Lee MG, Kang HH, Lee TR, Shin DW 
In:
PLoS ONE (2013) 8(7): e67604 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Malaquin N1, Vercamer C, Bouali F, Martien S, Deruy E, Wernert N, Chwastyniak M, Pinet F, Abbadie C, Pourtier A. 
In:
PLoS ONE (2013) 8(5): e63607 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Moreno Y Banuls L1, Katz A, Miklos W, Cimmino A, Tal DM, Ainbinder E, Zehl M, Urban E, Evidente A, Kopp B, Berger W, Feron O, Karlish S, Kiss R. 
In:
Mol Cancer (2013) 12: 33 
Categories:
Primary Cells and Media, Transfection 
Authors:
de Haro M, Al-Ramahi I, Jones KR, Holth JK, Timchenko LT, Botas J. 
In:
PLoS Genet (2013) 9(4): e1003445 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jurzak M, Adamczyk K 
In:
Acta Pol Pharm (2013) 70(2): 205-13 
Categories:
Primary Cells and Media 
Authors:
Chan ES, Liu H, Fernandez P, Luna A, Perez-Aso M, Bujor AM, Trojanowska M, Cronstein BN. 
In:
Arthritis Res Ther (2013) 15(3): R58 
Categories:
Primary Cells and Media 
Authors:
Janek T, Krasowska A, Radwanska A, Lukaszewicz M. 
In:
PLoS ONE (2013) 8(3): e57991 
Categories:
Primary Cells and Media 
Authors:
Otomo T1, Higaki K, Nanba E, Ozono K, Sakai N. 
In:
J Biol Chem (2011) 286(40): 35283-90 
Categories:
Transfection 
Authors:
Choi SM, Liu H, Chaudhari P, Kim Y, Cheng L, Feng J, Sharkis S, Ye Z, Jang YY 
In:
Blood (2011) 118(7): 1801-5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rao M1, Chinnasamy N, Hong JA, Zhang Y, Zhang M, Xi S, Liu F, Marquez VE, Morgan RA, Schrump DS. 
In:
Cancer Res (2011) 71(12): 4192-204 
Categories:
Primary Cells and Media 
Authors:
Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T 
In:
PLoS ONE (2011) 6(3): e17552 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yasuniwa Y, Izumi H, Wang KY, Shimajiri S, Sasaguri Y, Kawai K, Kasai H, Shimada T, Miyake K, Kashiwagi E, Hirano G, Kidani A, Akiyama M, Han B, Wu Y, Ieiri I, Higuchi S, Kohno K. 
In:
PLoS ONE (2010) 5(12): e15330 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang F, Waters KM, Miller JH, Gritsenko MA, Zhao R, Du X, Livesay EA, Purvine SO, Monroe ME, Wang Y, Camp DG 2nd, Smith RD, Stenoien DL. 
In:
PLoS ONE (2010) 5(11): e14152 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hoffmann M, Wu YJ, Gerber M, Berger-Rentsch M, Heimrich B, Schwemmle M, Zimmer G. 
In:
J Gen Virol (2010) 91(Pt 11): 2782-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Merfeld-Clauss S, Gollahalli N, March KL, Traktuev DO. 
In:
Tissue Eng (2010) 16(9): 2953-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T. 
In:
Cancer Res (2010) 70(14): 5829-39 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cole CL1, Hansen SU, Baráth M, Rushton G, Gardiner JM, Avizienyte E, Jayson GC. 
In:
PLoS ONE (2010) 5(7): e11644 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Corin KA, Gibson LJ. 
In:
Biomaterials (2010) 31(18): 4835-45 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Saffert RT, Penkert RR, Kalejta RF 
In:
J Virol (2010) 84(11): 5594-604 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chang KC1, Wang Y, Oh IG, Jenkins S, Freedman LP, Thompson CC, Chung JH, Nagpal S. 
In:
Mol Pharmacol (2010) 77(5): 744-50 
Categories:
Primary Cells and Media 
Authors:
Mongiat M, Marastoni S, Ligresti G, Lorenzon E, Schiappacassi M, Perris R, Frustaci S, Colombatti A. 
In:
Neoplasia (2010) 12(4): 294-304 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sylvest L1, Bendiksen CD, Houen G. 
In:
APMIS (2010) 118(1): 49-59 
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pilling D1, Fan T, Huang D, Kaul B, Gomer RH. 
In:
PLoS ONE (2009) 4(10): e7475 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao W1, Bover L, Cho M, Wen X, Hanabuchi S, Bao M, Rosen DB, Wang YH, Shaw JL, Du Q, Li C, Arai N, Yao Z, Lanier LL, Liu YJ. 
In:
J Exp Med (2009) 206(7): 1603-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin L1, Zhang JH, Panicker LM, Simonds WF. 
In:
Proc Natl Acad Sci USA (2008) 105(45): 17420-5 
Categories:
Transfection 
Authors:
Wally V, Klausegger A, Koller U, Lochmueller H, Krause S, Wiche G, Mitchell LG, Hintner H, Bauer JW 
In:
J Invest Dermatol (2008) 128(3): 568-74 
Categories:
Transfection 
Authors:
Kypriotou M, Beauchef G, Chadjichristos C, Widom R, Renard E, Jimenez S, Korn J, Maquart FX, Oddos T, Von Stetten O, Pujol JP, Galéra P 
In:
J Biol Chem (2007) 282(44): 32000-14 
Categories:
Transfection 
Authors:
Waterham HR, Koster J, van Roermund CW, Mooyer PA, Wanders RJ, Leonard JV 
In:
N Engl J Med (2007) 356(17): 1736-41 
Categories:
Transfection 
Authors:
Al-Mohanna MA, Al-Khalaf HH, Al-Yousef N, Aboussekhra A 
In:
Nucleic Acids Res (2007) 35(1): 223-233 
Categories:
Transfection 
Authors:
Pryor PR, Reimann F, Gribble FM, Luzio JP 
In:
Traffic (2006) 7(10): 1388-1398 
Categories:
Transfection 
Authors:
Fan X, Zhang H, Zhang S, Bagshaw RD, Tropak MB, Callahan JW, Mahuran DJ 
In:
Am J Hum Genet (2006) 79(4): 738-44 
Categories:
Transfection 
Authors:
Meng J, Ceryak S, Arastu Z, Jones L, Epstein L, Bouscarel B 
In:
Am J Physiol Cell Physiol (2006) 291(3): C546-54 
Categories:
Transfection 
Authors:
Pannu J, Gardner H, Shearstone JR, Smith E, Trojanowska M 
In:
Arthritis Rheum (2006) 54(9): 3011-21 
Categories:
Transfection 
Authors:
Le M, Krilov L, Meng J, Chapin-Kennedy K, Ceryak S, Bouscarel B 
In:
Am J Physiol Gastrointest Liver Physiol (2006) 291(2): G275-87 
Categories:
Transfection 
Authors:
Quan T, He T, Shao Y, Lin L, Kang S, Voorhees JJ, Fisher GJ 
In:
Am J Pathol (2006) 169(2): 482-90 
Categories:
Transfection 
Authors:
Jinnin M, Ihn H and Tamaki K 
In:
Mol Pharmacol (2006) 69(2): 597-607 
Categories:
Transfection 
Authors:
Balabanian K, Lagane B, Infantino S, Chow KY, Harriague J, Moepps B, Arenzana-Seisdedos F, Thelen M and Bachelerie F 
In:
J Biol Chem (2005) 280(42): 35760-35766 
Categories:
Transfection 
Authors:
Ashikov A, Routier F, Fuhlrott J, Helmus Y, Wild MK, Gerardy-Schahn R and Bakker H 
In:
J Biol Chem (2005) 280(29): 27230-27235 
Categories:
Transfection 
Authors:
Faucherre A, Desbois P, Nagano F, Satre V, Lunardi J, Gacon G and Dorseuil O 
In:
Hum Mol Genet (2005) 14(11): 1441-1448 
Categories:
Transfection 
Authors:
Verdijk P, Dijkman R, Plasmeijer EI, Mulder AA, Zoutman WH, Mieke Mommaas A, Tensen CP 
In:
J Invest Dermatol (2005) 124(4): 714-717 
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