Endothelial Cell Growth Medium

Endothelial cell growth media are low serum media optimized for the proliferation of endothelial cells.

EGM can be used to grow all of Clonetics Endothelial Cells except microvascular, coronary artery, umbilical artery, and iliac artery.

Final serum concentration is 2%
EGM is supplemented with Bovine Brain Extract (BBE)

The basal medium is also available without phenol red (CC-3129)

Shelf Life:
12 months
Recommended for Cells:
Endothelial, umbilical vein, human (HUVEC)
Categories:
Primary Cells and Media
Research Areas:
Cancer Research/Cell Biology
Cardiovascular
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Citations (36)

Regeneration of diaphragm with bio-3D cellular patch
Categories:
Primary Cells and Media 
Authors:
Zhang XY1, Yanagi Y2, Sheng Z3, Nagata K2, Nakayama K4, Taguchi T2 
In:
Biomaterials (2018) 167: 1-14 
Switch in Laminin ß2 to Laminin ß1 Isoforms During Aging Controls Endothelial Cell Functions-Brief Report
Categories:
Primary Cells and Media 
Authors:
Wagner JUG1,2, Chavakis E3, Rogg EM1, Muhly-Reinholz M1, Glaser SF1,2, Günther S4, John D1, Bonini F1, Zeiher AM3,2, Schaefer L5, Hannocks MJ2, Boon RA1,2, Dimmeler S6,2. 
In:
Arterioscler Thromb Vasc Biol (2018) 38: 1170-1177 
In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells.
Categories:
Primary Cells and Media 
Authors:
Hagiwara M1, Maruta N2,3, Marumoto M2 
In:
Tissue Eng (2017) 23: 323-332 
Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells.
Categories:
Primary Cells and Media 
Authors:
Ceserani V, Ferri A, Berenzi A, Benetti A, Ciusani E, Pascucci L, Bazzucchi C, Coccè V, Bonomi A, Pessina A, Ghezzi E, Zeira O, Ceccarelli P, Versari S, Tremolada C, Alessandri G 
In:
Other (2016) 8: 3 
Piperine Congeners as Inhibitors of Vascular Smooth Muscle Cell Proliferation.
Categories:
Primary Cells and Media 
Authors:
Mair CE, Liu R, Atanasov AG, Wimmer L, Nemetz-Fiedler D, Sider N, Heiss EH1, Mihovilovic MD, Dirsch VM1, Rollinger JM 
In:
Other (2015) ePub: ePub 
Viability and Functional Assessment of Murine Pancreatic Islets After Transportation Between Korea and Japan
Categories:
Primary Cells and Media 
Authors:
Lee S, Takahashi Y, Lee KM, Mizuno M, Nemeno JG, Takebe T, Lee JI. 
In:
Transplant Proc (2015) : 738-741 
Epidermal growth factor receptor inhibition reduces angiogenesis via hypoxia-inducible factor-1a and Notch1 in head neck squamous cell carcinoma.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang WM, Zhao ZL, Ma SR, Yu GT, Liu B, Zhang L, Zhang WF, Kulkarni AB, Sun ZJ, Zhao YF 
In:
PLoS ONE (2015) 10(2): e0119723 
Melanoma cells homing to the brain: an in vitro model
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizzo A, Vasco C, Girgenti V, Fugnanesi V, Calatozzolo C2, Canazza A, Salmaggi A, Rivoltini L, Morbin M, Ciusani E. 
In:
Biomed Res Int. (2015) 2015: 476069 
In vitro anti-proliferative and anti-angiogenic activities of thalidomide dithiocarbamate analogs
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
El-Aarag BY, Kasai T, Zahran MA, Zakhary NI, Shigehiro T, Sekhar SC, Agwa HS, Mizutani A, Murakami H, Kakuta H, Seno M 
In:
Int Immunol (2014) 21(2): 283-92 
Protective effect and localization by optical imaging of human renal CD133+ progenitor cells in an acute kidney injury model
Categories:
Primary Cells and Media 
Authors:
Grange C1, Moggio A, Tapparo M, Porta S, Camussi G, Bussolati B 
In:
Physiol Rep (2014) 2(5): e12009 
Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. 
In:
Circ Res (2014) 114(9): 1389-97 
Advanced glycation end products potentiate citrated plasma-evoked oxidative and inflammatory reactions in endothelial cells by up-regulating protease-activated receptor-1 expression
Categories:
Primary Cells and Media 
Authors:
Ishibashi Y, Matsui T, Ueda S, Fukami K, Yamagishi S 
In:
Cardiovasc Diabetol (2014) 13(1): 60 
Does the cis/trans configuration of peptide bonds in bioactive tripeptides play a role in ACE-1 enzyme inhibition?
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Siltari A, Viitanen R, Kukkurainen S, Vapaatalo H, Valjakka J 
In:
Other (2014) 8: 59-65 
Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant
Categories:
Primary Cells and Media 
Authors:
Takebe T, Zhang RR, Koike H, Kimura M, Yoshizawa E, Enomura M, Koike N, Sekine K3, Taniguchi H. 
In:
Nat Protocols (2014) 9(2): 396-409 
Therapeutic Benefit of Bone Marrow–Derived Endothelial Progenitor Cell Transplantation after Experimental Aneurysm Embolization with Coil in Rats
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang S, An Q, Li Q, Huang J, Chen X, Chen X, Zhang J, Wang Y, Yang GY, Zhu W. 
In:
PLoS ONE (2014) 9: 1-8 
Adenosine A2B receptors induce proliferation, invasion and activation of cAMP response element binding protein (CREB) in trophoblast cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Darashchonak N, Koepsell B, Bogdanova N, von Versen-Höynck F. 
In:
Biochem Biophys Res Commun (2014) 3: 14:2 
Biliverdins regulation of reactive oxygen species signalling leads to potent inhibition of proliferative and angiogenic pathways in head and neck cancer.
Categories:
Primary Cells and Media 
Authors:
Zheng J, Nagda DA, Lajud SA, Kumar S, Mouchli A, Bezpalko O 'Malley BW Jr, Li D. 
In:
Br J Cancer (2014) 110: 2116-2122 
CARDIOMYOGENIC DIFFERENTIATION OF FETAL DERIVED MESENCHYMAL STEM CELLS: A TRANSFORMATIVE APPROACH FOR CARDIAC TREATMENT
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shraddha Singh Gautam; Jaianand Kannaiyan 
In:
Int J Parma Med Bio Sci (2014) 3: 85-98 
Acute exposure to diesel exhaust impairs nitric oxide-mediated endothelial vasomotor function by increasing endothelial oxidative stress
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wauters A, Dreyfuss C, Pochet S, Hendrick P, Berkenboom G, van de Borne P, Argacha JF 
In:
Hypertension (2013) 62(2): 352-8 
AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takeuchi K, Morizane Y, Kamami-Levy C, Suzuki J, Kayama M, Cai W, Miller JW, Vavvas DG 
In:
J Biol Chem (2013) 288(28): 20581-91 
Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sawane M, Kajiya K, Kidoya H, Takagi M, Muramatsu F, Takakura N. 
In:
Diabetes (2013) 62(6): 1970-1980 
Celiac Disease-Specific TG2-Targeted Autoantibodies Inhibit Angiogenesis Ex Vivo and In Vivo in Mice by Interfering with Endothelial Cell Dynamics
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kalliokoski S, Sulic AM, Korponay-Szabó IR, Szondy Z, Frias R, Perez MA, Martucciello S, Roivainen A, Pelliniemi LJ, Esposito C, Griffin M, Sblattero D, Mäki M, Kaukinen K, Lindfors K, Caja S 
In:
PLoS ONE (2013) 8(6): e65887 
PRISM offers a comprehensive genomic approach to transcription factor function prediction
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wenger AM, Clarke SL, Guturu H, Chen J, Schaar BT, McLean CY, Bejerano G. 
In:
Genome Res (2013) 23(5): 889-904 
Exosome uptake depends on ERK1/2-heat shock protein 27 signaling and lipid Raft-mediated endocytosis negatively regulated by caveolin-1
Categories:
 
Authors:
Svensson KJ, Christianson HC, Wittrup A, Bourseau-Guilmain E, Lindqvist E, Svensson LM, Mörgelin M, Belting M 
In:
J Biol Chem (2013) 288(24): 17713-24 
Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92 cluster in endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kaluza D, Kroll J, Gesierich S, Manavski Y, Boeckel JN, Doebele C, Zelent A, Rössig L, Zeiher AM, Augustin HG, Urbich C, Dimmeler S 
In:
Arterioscler Thromb Vasc Biol (2013) 33(3): 533-43 
Noninvasive metabolic imaging of engineered 3D human adipose tissue in a perfusion bioreactor
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ward A, Quinn KP, Bellas E, Georgakoudi I, Kaplan DL 
In:
PLoS ONE (2013) 8(2): e55696 
Dual suppression of hemangiogenesis and lymphangiogenesis by splice-shifting morpholinos targeting vascular endothelial growth factor receptor 2 (KDR)
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uehara H, Cho Y, Simonis J, Cahoon J, Archer B, Luo L, Das SK, Singh N, Ambati J, Ambati BK 
In:
FASEB J (2013) 27(1): 76-85 
Nicotine-mediated induction of E-selectin in aortic endothelial cells requires Src kinase and E2F1 transcriptional activity
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alamanda V, Singh S, Lawrence NJ, Chellappan SP. 
In:
Biochem Biophys Res Commun (2012) 418(1): 56-61 
Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Salmanzadeh A, Romero L, Shafiee H, Gallo-Villanueva RC, Stremler MA, Cramer SD, Davalos RV. 
In:
Royal Soc of Chem (2012) 12: 182-189 
COMP-Ang1 stimulates HIF-1a-mediated SDF-1 overexpression and recovers ischemic injury through BM-derived progenitor cell recruitment.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Youn SW, Lee SW, Lee J, Jeong HK, Suh JW, Yoon CH, Kang HJ, Kim HZ, Koh GY, Oh BH, Park YB, Kim HS. 
In:
Blood (2011) 117(16): 4376-86 
Microtubule-disrupting chemotherapeutics result in enhanced proteasome-mediated degradation and disappearance of tubulin in neural cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Huff LM, Sackett DL, Poruchynsky MS, Fojo T. 
In:
Cancer Res (2010) 70(14): 5870-9 
A nitric oxide releasing, self assembled peptide amphiphile matrix that mimics native endothelium for coating implantable cardiovascular devices
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kushwaha M, Anderson JM, Bosworth CA, Andukuri A, Minor WP, Lancaster JR Jr, Anderson PG, Brott BC, Jun HW 
In:
Biomaterials (2010) 31(7): 1502-8 
Cellular pharmacokinetics and intracellular activity of torezolid (TR-700): studies with human macrophage (THP-1) and endothelial (HUVEC) cell lines.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lemaire S, Van Bambeke F, Appelbaum PC, Tulkens PM. 
In:
Other (2009) 64(5):: 1035-43 
First siRNA library screening in hard-to-transfect HUVEC cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Zumbansen M, Altrogge LM, Spottke NU, Spicker S, Offizier SM, Domzalski SB, St Amand AL, Toell A, Leake D, Mueller-Hartmann HA. 
In:
J RNAi Gene Silencing (2009) 6(1): 354-60 
Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cao W1, Bover L, Cho M, Wen X, Hanabuchi S, Bao M, Rosen DB, Wang YH, Shaw JL, Du Q, Li C, Arai N, Yao Z, Lanier LL, Liu YJ. 
In:
J Exp Med (2009) 206(7): 1603-14 
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