Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature.

Salmanzadeh A, Romero L, Shafiee H, Gallo-Villanueva RC, Stremler MA, Cramer SD, Davalos RV.
Source: Royal Soc of Chem
Publication Date: (2012)
Issue: 12: 182-189
Research Area:
Basic Research
Cells used in publication:
Pancreatic Islets, mouse
Species: mouse
Tissue Origin:
Prostate tumor initiating cells cultured in EGM.
In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.