Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature.
Authors:
Salmanzadeh A, Romero L, Shafiee H, Gallo-Villanueva RC, Stremler MA, Cramer SD, Davalos RV.
In:
Source:
Royal Soc of Chem
Publication Date:
(
2012
)
Issue:
12
:
182-189
Research Area:
Basic Research
Cells used in publication:
Pancreatic Islets, mouse
Species: mouse
Tissue Origin:
Culture Media:
Endothelial Cell Growth Medium
Experiment
Prostate tumor initiating cells cultured in EGM.
Abstract
In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.
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