Viability and Functional Assessment of Murine Pancreatic Islets After Transportation Between Korea and Japan
Lee S, Takahashi Y, Lee KM, Mizuno M, Nemeno JG, Takebe T, Lee JI.
Cells used in publication:
Pancreatic Islets, mouse
Endothelial Cell Growth Medium
Murine pancreatic islets cultured in EGM.
BACKGROUND: Organ donor scarcity remains a restricting factor for pancreatic islet transplantation. To date, limited information is available on the impact of long-distance transportation on transplantable pancreatic islets. The objective of this study was to assess the effects of transportation on the viability and function of murine pancreatic islet cells. METHODS: The isolated murine pancreatic islets were transported from Japan to Korea with the use of commercial modes of transportation: subway and commercial airplane. After transportation, the islets were assessed by performing a viability assay and by evaluating the islets' insulin secretion in response to glucose stimulation. A comparative study was performed for evaluating the insulin secretory responses of transported and control islets (not transported). RESULTS: There was no evidence of contamination in the transported pancreatic islets. No significant differences were observed in the viability and functionality of the transported and control islet cells. CONCLUSIONS: These findings show the feasibility of pancreatic islet transportation from Japan to Korea. Our data could be used not only for the inter-Asian but also for global advancement of animal and human islet transportation methods and transplantation research.
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