While, cells may be able to be cultured in TheraPEAK® Mesenchymal Stem Cell Growth Medium (TheraPeak® MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate coatings may also be acceptable. An extracellular matrix/plate coating is especially recommended for cells in the first passage out of cryopreservation and when transitioning cells from serum containing media to TheraPEAK® Mesenchymal Stem Cell Growth Medium (TheraPeak® MSCGM™).