Adipose stem cell, human normal

Multipotent stem cells isolated from stroma-vascular fraction of infant fat

Cell Type:
Misc. Somatic SC
Tissue Origin:
adipose
Species:
human
Research Area:
Dermatology/Tissue Engineering
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

Media for differentiating MSC into osteoblasts

Media for the differentiation of hMSC cells into adipocytes.

This kit consists of two media:
hMSC Adipogenic Induction Medium (consisting of  basal medium (PT-3102B) and Singlequots (PT-4135)
hMSC Adipogenic Maintenance Medium (consisting of basal medium (PT-3102A) and SingleQuots (PT-4122)

TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth Medium, Serum-free (with L-glutamine, without phenol red and antibiotics).

TheraPEAK™MSCGM-CD™ is a serum free medium for the growth of human Mesenchymal Stem Cells. MSCGM-CD™ is optimized for the multiple passage expansion of all types of hMSCs while still allowing for differentiation into the several desired lineages. Cells can be directly transitioned from serum-containing medium to MSCGM-CD™ with little to no adaptation time. For peak performance, it is recommended to use MSCGM-CD™ with Fibronectin coated plates



Optimised for growth and expansion of Human Adipose-Derived Stem Cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
MSC, human U-023 6e5 76% Plasmid (general) 3 µg 100 µl I/II/2b
P1 DO-101 2e5 91% 73-75% Plasmid (general) 1 µg 20 µl 4D X-Unit
P1 DO-101 2e6 94% 72% Plasmid (general) 5 µg 100 µl 4D X-Unit

Citations (25)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Deng M, Gu Y, Liu Z, Qi Y, Ma GE, Kang N 
In:
Stem Cells Intl (2015) 2015: 350718 
Categories:
 
Authors:
Gandhimathi C, Edwin NXH, Jayaraman P, Venugopal JR, Ramakrishna S, Kuma SD 
In:
Other (2015) 6(2): 1-7 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rohringer S, Hofbauer P, Schneider KH, Husa AM, Feichtinger G, Peterbauer-Scherb A, Redl H, Holnthoner W. 
In:
Angiogenesis (2014) 17(4): 921-33 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Griffeth RJ, García-Párraga D, Mellado-López M, Crespo-Picazo JL, Soriano-Navarro M4, Martinez-Romero A, Moreno-Manzano V 
In:
PLoS ONE (2014) 9(9): ePub 
Categories:
Primary Cells and Media 
Authors:
Falcon BL, Swearingen M, Gough WH, Lee L, Foreman R, Uhlik M, Hanson JC, Lee JA, McClure DB, Chintharlapalli S 
In:
PLoS ONE (2014) 9(9): ePub 
Categories:
Primary Cells and Media 
Authors:
Rauch C, Wechselberger J, Feifel E, Gstraunthaler G 
In:
Other (2014) 2(1): 1-11 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lopatina T, Bruno S, Tetta C, Kalinina N, Porta M, Camussi G 
In:
Cell Commun Signal (2014) 12: ePub 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rojas-Rodriguez R, Gealekman O, Kruse ME, Rosenthal B, Rao K, Min S, Bellve KD, Lifshitz LM, Corvera S 
In:
Methods Enzymol (2014) 537: 75-91 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Navarro A, Marín S, Riol N, Carbonell-Uberos F, Miñana MD 
In:
Stem Cell Res Ther (2014) 5(2): 50 
Categories:
Primary Cells and Media 
Authors:
Kimmel J, Sugaya K 
In:
Other (2014) 4(1): 2210-2973 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Farb MG, Tiwari S, Karki S, Ngo DT, Carmine B, Hess DT, Zuriaga MA, Walsh K, Fetterman JL, Hamburg NM, Vita JA, Apovian CM, Gokce N. 
In:
Obesity (2014) 22(2): 349-55 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shi Z, Neoh KG, Kang ET, Poh CK, Wang W. 
In:
Other (2014) 8(1): 50-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mihaila SM, Frias AM, Pirraco RP, Rada T, Reis RL, Gomes ME, Marques AP 
In:
Tissue Eng (2013) 19(1-2): 235-46 
Categories:
Primary Cells and Media 
Authors:
Li X, Pham HT, Janesick AS, Blumberg B. 
In:
Other (2012) 120(12): 1720-6 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chandler EM, Seo BR, Califano JP, Andresen Eguiluz RC, Lee JS, Yoon CJ, Tims DT, Wang JX, Cheng L, Mohanan S, Buckley MR, Cohen I, Nikitin AY, Williams RM, Gourdon D, Reinhart-King CA, Fischbach C. 
In:
Proc Natl Acad Sci USA (2012) 109(25): 9786-91 
Categories:
Primary Cells and Media 
Authors:
Willard MD, Lajiness ME, Wulur IH, Feng B, Swearingen ML, Uhlik MT, Kinzler KW, Velculescu VE, Sjöblom T, Markowitz SD, Powell SM, Vogelstein B, Barber TD. 
In:
Mol Cancer Res (2012) 10(6): 739-49 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Meier T, Uhlik M, Chintharlapalli S, Dowless M, Van Horn R, Stewart J, Blosser W, Cook J, Young D, Ye X, Evans G, Credille K, Ballard D, Huber L, Capen A, Chedid M, Ilaria R Jr, Smith MC, Stancato L. 
In:
Mol Cancer Ther (2011) 10(11): 2168-78 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chandler EM, Saunders MP, Yoon CJ, Gourdon D, Fischbach C. 
In:
Other (2011) 8(1): 015008 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gealekman O, Guseva N, Hartigan C, Apotheker S, Gorgoglione M, Gurav K, Tran KV, Straubhaar J, Nicoloro S, Czech MP, Thompson M, Perugini RA, Corvera S 
In:
Circulation (2011) 123(2): 186-94 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rich JT, Rosová I, Nolta JA, Myckatyn TM, Sandell LJ, McAlinden A. 
In:
Biochem Biophys Res Commun (2008) 372(1): 230-5 
Categories:
Transfection 
Authors:
Ugland H, Boquest AC, Naderi S, Collas P, Blomhoff HK 
In:
Mol Biol Cell (2008) epub: online 
Categories:
Transfection 
Authors:
Zaragosi LE, Wdziekonski B, Fontaine C, Villageois P, Peraldi P, Dani C 
In:
BMC Cell Biol (2008) 9(1): 11 
Categories:
Transfection 
Authors:
Zaragosi LE, Billon N, Ailhaud G, Dani C 
In:
Stem Cells (2007) 25(3): 790-7 
Categories:
Primary Cells and Media 
Authors:
Kuss MA1,2, Wu S1,2, Wang Y1,2, Untrauer JB3, Li W4, Lim JY1,4, Duan B1,2,5. 
In:
J Biomed Mater Res A () 106: 1788-1798 
+ Show All