Adipose stem cell, human diabetes Tp.1

Diabetes mellitus type 1 (Type 1 diabetes, IDDM, or, formerly, juvenile diabetes) is a form of diabetes mellitus that results from autoimmune destruction of insulin-producing beta cells of the pancreas. It can be treated with Insulin injections.

Cell Type:
Misc. Somatic SC
Tissue Origin:
adipose
Species:
human
Research Area:
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

Media for differentiating MSC into osteoblasts

Media for the differentiation of hMSC cells into adipocytes.

This kit consists of two media:
hMSC Adipogenic Induction Medium (consisting of  basal medium (PT-3102B) and Singlequots (PT-4135)
hMSC Adipogenic Maintenance Medium (consisting of basal medium (PT-3102A) and SingleQuots (PT-4122)

TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth Medium, Serum-free (with L-glutamine, without phenol red and antibiotics).

TheraPEAK™MSCGM-CD™ is a serum free medium for the growth of human Mesenchymal Stem Cells. MSCGM-CD™ is optimized for the multiple passage expansion of all types of hMSCs while still allowing for differentiation into the several desired lineages. Cells can be directly transitioned from serum-containing medium to MSCGM-CD™ with little to no adaptation time. For peak performance, it is recommended to use MSCGM-CD™ with Fibronectin coated plates



Optimised for growth and expansion of Human Adipose-Derived Stem Cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1 DO-101 2e5 90% 66% Plasmid (general) 1 µg 20 µl 4D X-Unit
P1 DO-101 2e6 92% 58% Plasmid (general) 5 µg 100 µl 4D X-Unit