Q:

Following Nucleofection®, I cannot obtain stable clones from single cells. What could be the reason?

A:

Some cell lines need certain cell densities to proliferate.

For those cells you can try using conditioned medium for setting up limiting dilution or culturing single cells together with feeder cells.

Related Literature:
Lonza_BenchGuides_Guideline_for_Generation_of_Stable_Cell_Lines__Technical_Reference_Guide.pdf
Categories:
Transfection
Research Areas:
Gene Expression

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