Neuron, hippocampal, rat

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Neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley or Fischer 344 rat brain.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, rat C-013 1e6 50% good 2 µg 100 µl I/II/2b

Citations (37)

Pharmacological or genetic depletion of senescent astrocytes prevents whole brain irradiation-induced impairment of neurovascular coupling responses protecting cognitive function in mice
Categories:
Primary Cells and Media 
Authors:
Yabluchanskiy A, Tarantini S, Balasubramanian P, Kiss T, Csipo T, Fülöp GA, Lipecz A, Ahire C, DelFavero J, Nyul-Toth A, Sonntag WE, Schwartzman ML, Campisi J, Csiszar A, Ungvari Z 
In:
Geroscience (2020) :  
The TRPM8 Protein Is a Testosterone Receptor: II. FUNCTIONAL EVIDENCE FOR AN IONOTROPIC EFFECT OF TESTOSTERONE ON TRPM8
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Asuthkar S, Demirkhanyan L, Sun X, Elustondo PA, Krishnan V, Baskaran P, Velpula KK, Thyagarajan B, Pavlov EV, Zakharian E. 
In:
J Biol Chem (2015) 290(5): 2670-88 
Melanoma cells homing to the brain: an in vitro model
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizzo A, Vasco C, Girgenti V, Fugnanesi V, Calatozzolo C2, Canazza A, Salmaggi A, Rivoltini L, Morbin M, Ciusani E. 
In:
Biomed Res Int. (2015) 2015: 476069 
The human language and epilepsy associated gene SRPX2 regulates synapse formation and vocalization in mice
Categories:
Transfection 
Authors:
Sia GM, Clem RL, Huganir RL. 
In:
Science (2013) 342: 987-991 
S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade.
Categories:
Primary Cells and Media 
Authors:
Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. 
In:
J Biol Chem (2008) 283(27): 18478-82 
Carbon nanotube fibers are compatible with Mammalian cells and neurons.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Dubin RA, Callegari G, Kohn J, Neimark A. 
In:
IEEE Transactions on NanoBioscience (2008) 7(1): 11-14 
FMRP phosphorylation reveals an immediate-early signaling pathway triggered by group I mGluR and mediated by PP2A.
Categories:
Primary Cells and Media 
Authors:
Narayanan U, Nalavadi V, Nakamoto M, Pallas DC, Ceman S, Bassell GJ, Warren ST. 
In:
J Neurosci (2007) 27(52): 14349-57 
Magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain
Categories:
Primary Cells and Media 
Authors:
Manganas LN, Zhang X, Li Y, Hazel RD, Smith SD, Wagshul ME, Henn F, Benveniste H, Djuric PM, Enikolopov G, Maletic-Savatic M. 
In:
Science (2007) 318(5852): 980-5 
Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth
Categories:
Transfection 
Authors:
Hisata S, Sakisaka T, Baba T, Yamada T, Aoki K, Matsuda M, Takai Y 
In:
J Cell Biol (2007) 178(5): 843-60 
Tau-dependent microtubule disassembly initiated by prefibrillar -amyloid
Categories:
Transfection 
Authors:
King ME, Kan HM, Baas PW, Erisir A, Glabe CG, Bloom GS 
In:
J Cell Biol (2006) 175(4): 541-6 
Molecular network and chromosomal clustering of genes involved in synaptic plasticity in the hippocampus
Categories:
Transfection 
Authors:
Park CS, Gong R, Stuart J, Tang SJ 
In:
J Biol Chem (2006) 281(40): 30195-211 
Drug-induced activation of SREBP-controlled lipogenic gene expression in CNS-related cell lines: marked differences between various antipsychotic drugs
Categories:
Primary Cells and Media 
Authors:
Fernø J, Skrede S, Vik-Mo AO, Håvik B, Steen VM. 
In:
BMC Neurosci (2006) 7: 69 
Regulation of neuronal morphology by toca-1, an F-BAR/EFC protein that induces plasma membrane invagination
Categories:
Transfection 
Authors:
Kakimoto T, Katoh H, Negishi M 
In:
J Biol Chem (2006) 281(39): 29042-53 
SNAP-25/syntaxin 1A complex functionally modulates neurotransmitter GABA reuptake
Categories:
Transfection 
Authors:
Fan HP, Fan FJ, Bao L, Pei G 
In:
J Biol Chem (2006) 281(38): 28174-84 
Activated c-Jun N-terminal kinase is required for axon formation
Categories:
Transfection 
Authors:
Oliva AA Jr, Atkins CM, Copenagle L, Banker GA 
In:
J Neurosci (2006) 26(37): 9462-70 
The Rac Activator DOCK7 Regulates Neuronal Polarity through Local Phosphorylation of Stathmin/Op18
Categories:
Transfection 
Authors:
Watabe-Uchida M, John KA, Janas JA, Newey SE, Van Aelst L 
In:
Neuron (2006) 51(6): 727-39 
Anaplastic lymphoma kinase is a dependence receptor whose proapoptotic functions are activated by caspase cleavage
Categories:
Transfection 
Authors:
Mourali J, Benard A, Lourenco FC, Monnet C, Greenland C, Moog-Lutz C, Racaud-Sultan C, Gonzalez-Dunia D, Vigny M, Mehlen P, Delsol G, Allouche M 
In:
Mol Cell Biol (2006) 26(16): 6209-22 
Interneurite affinity is regulated by heterophilic nectin interactions in concert with the cadherin machinery
Categories:
Transfection 
Authors:
Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M 
In:
J Cell Biol (2006) 174(1): 141-51 
Sema4D/plexin-B1 activates GSK-3beta through R-Ras GAP activity, inducing growth cone collapse
Categories:
Transfection 
Authors:
Ito Y, Oinuma I, Katoh H, Kaibuchi K, Negishi M 
In:
EMBO Rep (2006) 7(7): 704-9 
Palmitoylation of huntingtin by HIP14is essential for its trafficking and function
Categories:
Transfection 
Authors:
Yanai A, Huang K, Kang R, Singaraja RR, Arstikaitis P, Gan L, Orban PC, Mullard A, Cowan CM, Raymond LA, Drisdel RC, Green WN, Ravikumar B, Rubinsztein DC, El-Husseini A, Hayden MR 
In:
Nat Neurosci (2006) 9(6): 824-831 
Regulation of NMDA receptor trafficking and function by striatal-enriched tyrosine phosphatase (STEP)
Categories:
Transfection 
Authors:
Braithwaite SP, Adkisson M, Leung J, Nava A, Masterson B, Urfer R, Oksenberg D, Nikolich K 
In:
Eur J Neurosci (2006) 23(11): 2847-56 
Effects of dynactin disruption and Dynein depletion on axonal microtubules
Categories:
Transfection 
Authors:
Ahmad FJ, He Y, Myers KA, Hasaka TP, Francis F, Black MM, Baas PW 
In:
Traffic (2006) 7(5): 524-37 
Microtubule affinity-regulating kinase 2 functions downstream of the PAR-3/PAR-6/atypical PKC complex in regulating hippocampal neuronal polarity
Categories:
Transfection 
Authors:
Chen YM, Wang QJ, Hu HS, Yu PC, Zhu J, Drewes G, Piwnica-Worms H, Luo ZG 
In:
Proc Natl Acad Sci USA (2006) 103(22): 8534-9 
Cell Survival through Trk Neurotrophin Receptors Is Differentially Regulated by Ubiquitination
Categories:
Transfection 
Authors:
Arevalo JC, Waite J, Rajagopal R, Beyna M, Chen ZY, Lee FS, Chao MV 
In:
Neuron (2006) 50(4): 549-59 
SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development
Categories:
Transfection 
Authors:
Zhu PP, Soderblom C, Tao-Cheng JH, Stadler J, Blackstone C 
In:
Hum Mol Genet (2006) 15(8): 1343-53 
Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
Categories:
Transfection 
Authors:
Loomis PA, Kelly AE, Zheng L, Changyaleket B, Sekerkova G, Mugnaini E, Ferreira A, Mullins RD, Bartles JR 
In:
J Cell Sci (2006) 119(Pt 8): 1655-65 
Ligand-Dependent Cleavage of the P75 Neurotrophin Receptor Is Necessary for NRIF Nuclear Translocation and Apoptosis in Sympathetic Neurons
Categories:
Transfection 
Authors:
Kenchappa RS, Zampieri N, Chao MV, Barker PA, Teng HK, Hempstead BL, Carter BD 
In:
Neuron (2006) 50(2): 219-32 
cAMP-dependent protein kinase postsynaptic localization regulated by NMDA receptor activation through translocation of an A-kinase anchoring protein scaffold protein
Categories:
Transfection 
Authors:
Smith KE, Gibson ES, Dell'Acqua ML 
In:
J Neurosci (2006) 26(9): 2391-402 
Tau protects microtubules in the axon from severing by katanin
Categories:
Transfection 
Authors:
Qiang L, Yu W, Andreadis A, Luo M, Baas PW 
In:
J Neurosci (2006) 26(12): 3120-9 
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling
Categories:
Transfection 
Authors:
Volmer R, Monnet C, Gonzalez-Dunia D 
In:
PLoS Pathog (2006) 2(3): e19 
Alternative splicing of human metabotropic glutamate receptor 3
Categories:
Transfection 
Authors:
Sartorius LJ, Nagappan G, Lipska BK, Lu B, Sei Y, Ren-Patterson R, Li Z, Weinberger DR, Harrison PJ 
In:
J Neurochem (2006) 96(4): 1139-48 
Functional analysis of SIRP in the growth cone
Categories:
Transfection 
Authors:
Wang XX and Pfenninger KH 
In:
J Cell Sci (2006) 119(Pt 1): 172-183 
Nucleofection of primary neurons
Categories:
Transfection 
Authors:
Gartner A, Collin L, Lalli G 
In:
Methods Enzymol (2006) 406: 374-88 
The BTB domain of the nuclear matrix protein NRP/B is required for neurite outgrowth
Categories:
Transfection 
Authors:
Kim TA, Jiang S, Seng S, Cha K, Avraham HK and Avraham S 
In:
J Cell Sci (2005) 118(Pt 23): 5537-5548 
Distinct enzyme combinations in AKAP signalling complexes permit functional diversity
Categories:
Transfection 
Authors:
Hoshi N, Langeberg LK and Scott JD 
In:
Nat Cell Biol (2005) 7(11): 1066-1073 
Par-4 links dopamine signaling and depression
Categories:
Transfection 
Authors:
Park SK, Nguyen MD, Fischer A, Luke MP, Affar el B, Dieffenbach PB, Tseng HC, Shi Y and Tsai LH 
In:
Cell (2005) 122(2): 275-287 
Nuclear calpain regulates Ca2+-dependent signaling via proteolysis of nuclear Ca2+/calmodulin-dependent protein kinase type IV in cultured neurons.
Categories:
Primary Cells and Media 
Authors:
Tremper-Wells B, Vallano ML. 
In:
J Biol Chem (2005) 280(3: 2165-75 
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