Neuron, hippocampal, rat

Neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley or Fischer 344 rat brain.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, rat C-013 1e6 50% good 2 µg 100 µl I/II/2b

Citations (36)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Asuthkar S, Demirkhanyan L, Sun X, Elustondo PA, Krishnan V, Baskaran P, Velpula KK, Thyagarajan B, Pavlov EV, Zakharian E. 
In:
J Biol Chem (2015) 290(5): 2670-88 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizzo A, Vasco C, Girgenti V, Fugnanesi V, Calatozzolo C2, Canazza A, Salmaggi A, Rivoltini L, Morbin M, Ciusani E. 
In:
Biomed Res Int. (2015) 2015: 476069 
Categories:
Transfection 
Authors:
Sia GM, Clem RL, Huganir RL. 
In:
Science (2013) 342: 987-991 
Categories:
Primary Cells and Media 
Authors:
Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. 
In:
J Biol Chem (2008) 283(27): 18478-82 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Dubin RA, Callegari G, Kohn J, Neimark A. 
In:
IEEE Transactions on NanoBioscience (2008) 7(1): 11-14 
Categories:
Primary Cells and Media 
Authors:
Narayanan U, Nalavadi V, Nakamoto M, Pallas DC, Ceman S, Bassell GJ, Warren ST. 
In:
J Neurosci (2007) 27(52): 14349-57 
Categories:
Primary Cells and Media 
Authors:
Manganas LN, Zhang X, Li Y, Hazel RD, Smith SD, Wagshul ME, Henn F, Benveniste H, Djuric PM, Enikolopov G, Maletic-Savatic M. 
In:
Science (2007) 318(5852): 980-5 
Categories:
Transfection 
Authors:
Hisata S, Sakisaka T, Baba T, Yamada T, Aoki K, Matsuda M, Takai Y 
In:
J Cell Biol (2007) 178(5): 843-60 
Categories:
Transfection 
Authors:
King ME, Kan HM, Baas PW, Erisir A, Glabe CG, Bloom GS 
In:
J Cell Biol (2006) 175(4): 541-6 
Categories:
Transfection 
Authors:
Park CS, Gong R, Stuart J, Tang SJ 
In:
J Biol Chem (2006) 281(40): 30195-211 
Categories:
Primary Cells and Media 
Authors:
Fernø J, Skrede S, Vik-Mo AO, Håvik B, Steen VM. 
In:
BMC Neurosci (2006) 7: 69 
Categories:
Transfection 
Authors:
Kakimoto T, Katoh H, Negishi M 
In:
J Biol Chem (2006) 281(39): 29042-53 
Categories:
Transfection 
Authors:
Fan HP, Fan FJ, Bao L, Pei G 
In:
J Biol Chem (2006) 281(38): 28174-84 
Categories:
Transfection 
Authors:
Oliva AA Jr, Atkins CM, Copenagle L, Banker GA 
In:
J Neurosci (2006) 26(37): 9462-70 
Categories:
Transfection 
Authors:
Watabe-Uchida M, John KA, Janas JA, Newey SE, Van Aelst L 
In:
Neuron (2006) 51(6): 727-39 
Categories:
Transfection 
Authors:
Mourali J, Benard A, Lourenco FC, Monnet C, Greenland C, Moog-Lutz C, Racaud-Sultan C, Gonzalez-Dunia D, Vigny M, Mehlen P, Delsol G, Allouche M 
In:
Mol Cell Biol (2006) 26(16): 6209-22 
Categories:
Transfection 
Authors:
Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M 
In:
J Cell Biol (2006) 174(1): 141-51 
Categories:
Transfection 
Authors:
Ito Y, Oinuma I, Katoh H, Kaibuchi K, Negishi M 
In:
EMBO Rep (2006) 7(7): 704-9 
Categories:
Transfection 
Authors:
Yanai A, Huang K, Kang R, Singaraja RR, Arstikaitis P, Gan L, Orban PC, Mullard A, Cowan CM, Raymond LA, Drisdel RC, Green WN, Ravikumar B, Rubinsztein DC, El-Husseini A, Hayden MR 
In:
Nat Neurosci (2006) 9(6): 824-831 
Categories:
Transfection 
Authors:
Braithwaite SP, Adkisson M, Leung J, Nava A, Masterson B, Urfer R, Oksenberg D, Nikolich K 
In:
Eur J Neurosci (2006) 23(11): 2847-56 
Categories:
Transfection 
Authors:
Ahmad FJ, He Y, Myers KA, Hasaka TP, Francis F, Black MM, Baas PW 
In:
Traffic (2006) 7(5): 524-37 
Categories:
Transfection 
Authors:
Chen YM, Wang QJ, Hu HS, Yu PC, Zhu J, Drewes G, Piwnica-Worms H, Luo ZG 
In:
Proc Natl Acad Sci USA (2006) 103(22): 8534-9 
Categories:
Transfection 
Authors:
Arevalo JC, Waite J, Rajagopal R, Beyna M, Chen ZY, Lee FS, Chao MV 
In:
Neuron (2006) 50(4): 549-59 
Categories:
Transfection 
Authors:
Zhu PP, Soderblom C, Tao-Cheng JH, Stadler J, Blackstone C 
In:
Hum Mol Genet (2006) 15(8): 1343-53 
Categories:
Transfection 
Authors:
Loomis PA, Kelly AE, Zheng L, Changyaleket B, Sekerkova G, Mugnaini E, Ferreira A, Mullins RD, Bartles JR 
In:
J Cell Sci (2006) 119(Pt 8): 1655-65 
Categories:
Transfection 
Authors:
Kenchappa RS, Zampieri N, Chao MV, Barker PA, Teng HK, Hempstead BL, Carter BD 
In:
Neuron (2006) 50(2): 219-32 
Categories:
Transfection 
Authors:
Smith KE, Gibson ES, Dell'Acqua ML 
In:
J Neurosci (2006) 26(9): 2391-402 
Categories:
Transfection 
Authors:
Qiang L, Yu W, Andreadis A, Luo M, Baas PW 
In:
J Neurosci (2006) 26(12): 3120-9 
Categories:
Transfection 
Authors:
Volmer R, Monnet C, Gonzalez-Dunia D 
In:
PLoS Pathog (2006) 2(3): e19 
Categories:
Transfection 
Authors:
Sartorius LJ, Nagappan G, Lipska BK, Lu B, Sei Y, Ren-Patterson R, Li Z, Weinberger DR, Harrison PJ 
In:
J Neurochem (2006) 96(4): 1139-48 
Categories:
Transfection 
Authors:
Wang XX and Pfenninger KH 
In:
J Cell Sci (2006) 119(Pt 1): 172-183 
Categories:
Transfection 
Authors:
Gartner A, Collin L, Lalli G 
In:
Methods Enzymol (2006) 406: 374-88 
Categories:
Transfection 
Authors:
Kim TA, Jiang S, Seng S, Cha K, Avraham HK and Avraham S 
In:
J Cell Sci (2005) 118(Pt 23): 5537-5548 
Categories:
Transfection 
Authors:
Hoshi N, Langeberg LK and Scott JD 
In:
Nat Cell Biol (2005) 7(11): 1066-1073 
Categories:
Transfection 
Authors:
Park SK, Nguyen MD, Fischer A, Luke MP, Affar el B, Dieffenbach PB, Tseng HC, Shi Y and Tsai LH 
In:
Cell (2005) 122(2): 275-287 
Categories:
Primary Cells and Media 
Authors:
Tremper-Wells B, Vallano ML. 
In:
J Biol Chem (2005) 280(3: 2165-75 
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