Endothelial, aortic, human (HAEC)

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Endothelial cells isolated from human aorta

Cell Type:
Aortic Endo.
Tissue Origin:
aortic
Species:
human
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Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial Cell Growth Medium 2

Endothelial cell media are low serum media optimized for the proliferation of endothelial cells.

EGM-2 is a refined version of EGM.
 - Does not contain BBE
 - Final serum concentration is 2%
 - Improved cell proliferation over EGM

EGM-2 can be used to grow all of Clonetics Endothelial Cells except microvascular, coronary artery, umbilical artery, and iliac artery

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P5

EH-100

 1e5 73% 70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P5 EH-100 1e5 73% ±8 70% ±8 Plasmid (general) 0.4 µg 20 µl 96-well
AD1 EG-142 1e5 85% 85% Plasmid (general) 17 µg 350 µl 4D Y-Unit

Citations (51)

Altered COVID-19 Receptor ACE2 Expression in a Higher Risk Group for Cerebrovascular Disease and Ischemic Stroke
Categories:
Primary Cells and Media 
Authors:
Choi JY, Lee HK, Park JH, Cho SJ, Kwon M, Jo C, Koh YH 
In:
Biochem Biophys Res Commun (2020) 528(3): 413-9 
Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs
Categories:
Primary Cells and Media 
Authors:
Alfonso-Garcia A1, Shklover J1, Sherlock BE1, Panitch A1, Griffiths LG2, Marcu L1 
In:
Other (2018) 9: e201700391 
Blueberry Metabolites Attenuate Lipotoxicity-Induced Endothelial Dysfunction.
Categories:
Primary Cells and Media 
Authors:
Bharat D1, Cavalcanti RRM1, Petersen C1, Begaye N1, Cutler BR1, Costa MMA1, Ramos RKLG1, Ferreira MR1, Li Y1, Bharath LP1, Toolson E1, Sebahar P2, Looper RE2, Jalili T1, Rajasekaran NS3, Jia Z4, Symons JD1,5, Anandh Babu PV1 
In:
Mol Nutr Food Res (2018) 62:  
A biomimetic microfluidic model to study signalling between endothelial and vascular smooth muscle cells under hemodynamic conditions
Categories:
Primary Cells and Media, Cell Lines and Primary Cancer Cells 
Authors:
van Engeland NCA1, Pollet AMAO, den Toonder JMJ, Bouten CVC, Stassen OMJA, Sahlgren CM 
In:
Lab Chip (2018) 11: 1607-1620 
The chemokine receptor CX3CR1 coordinates monocyte recruitment and endothelial regeneration after arterial injury.
Categories:
Primary Cells and Media 
Authors:
Getzin T1, Krishnasamy K1,2, Gamrekelashvili J1,2, Kapanadze T1,2, Limbourg A1, Häger C1, Napp LC1,3, Bauersachs J3, Haller H2, Limbourg FP4,2 
In:
EMBO Mol Med (2017) 10(2): 151-159 
Donepezil can improve ischemic muscle atrophy by activating angiomyogenic properties of satellite cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Noguchi T, Kakinuma Y, Arikawa M, Okazaki K, Hoshino E, Iiyama T, Kubo T, Kitaoka H, Doi Y, Sato T 
In:
Circ J. (2014) 78(9): 2317-24 
Toll-like receptor 4 mediates endothelial cell activation through NF-?B but is not associated with endothelial dysfunction in patients with rheumatoid arthritis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Menghini R, Campia U, Tesauro M, Marino A, Rovella V, Rodia G, Schinzari F, Tolusso B, di Daniele N, Federici M, Zoli A, Ferraccioli G, Cardillo C 
In:
PLoS ONE (2014) 9(6): e99053 
Dimethylfumarate attenuates restenosis after acute vascular injury by cell-specific and Nrf2-dependent mechanisms
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Oh CJ, Park S, Kim JY, Kim HJ, Jeoung NH, Choi YK1, Go Y, Park KG, Lee IK 
In:
Redox Biol. (2014) 2: 855-64 
Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. 
In:
Circ Res (2014) 114(9): 1389-97 
Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jung CH, Lee MJ, Kang YM, Lee YL, Yoon HK, Kang SW, Lee WJ, Park JY 
In:
Cardiovasc Diabetol (2014) 13: 41 
MiR-216a: a link between endothelial dysfunction and autophagy.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Menghini R1, Casagrande V1, Marino A1, Marchetti V1, Cardellini M1, Stoehr R1, Rizza S1, Martelli E2, Greco S3, Mauriello A2, Ippoliti A2, Martelli F3, Lauro R1, Federici M1. 
In:
Cell Death Dis. (2014) 5: e1029 
Mycophenolic acid attenuates the tumour necrosis factor-a-mediated proinflammatory response in endothelial cells by blocking the MAPK/NF-?B and ROS pathways
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Olejarz W, Bryk D, Zapolska-Downar D, Malecki M, Stachurska A, Sitkiewicz D 
In:
Eur J Clin Invest (2014) 44(1): 54-64 
SLIT3-ROBO4 activation promotes vascular network formation in human engineered tissue and angiogenesis in vivo
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Paul JD, Coulombe KL, Toth PT, Zhang Y, Marsboom G, Bindokas VP, Smith DW, Murry CE, Rehman J 
In:
J Mol Cell Cardiol (2013) 64: 124-31 
Electronegative low-density lipoprotein increases C-reactive protein expression in vascular endothelial cells through the LOX-1 receptor
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chu CS, Wang YC, Lu LS, Walton B, Yilmaz HR, Huang RY, Sawamura T, Dixon RA, Lai WT, Chen CH, Lu J 
In:
PLoS ONE (2013) 8 (8): e70533 
NADPH Oxidase 1 Plays a Key Role in Diabetes Mellitus-Accelerated Atherosclerosis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gray SP, Di Marco E, Okabe J, Szyndralewiez C, Heitz F, Montezano AC, de Haan JB, Koulis C, El-Osta A, Andrews KL, Chin-Dusting JP, Touyz RM, Wingler K, Cooper ME, Schmidt HH, Jandeleit-Dahm KA. 
In:
Circulation (2013) 127(18): 1888-902 
Toll-like receptor 2 mediates high-fat diet-induced impairment of vasodilator actions of insulin
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jang HJ, Kim HS, Hwang DH, Quon MJ, Kim JA 
In:
Am J Physiol Endocrinol Metab (2013) 304(10): e1077-88 
SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and senescence
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cardus A, Uryga AK, Walters G, Erusalimsky JD. 
In:
Cardiovasc Res (2013) 97(3): 571-9 
Insulin-like growth factor-1 regulates glutathione peroxidase expression and activity in vascular endothelial cells: Implications for atheroprotective actions of insulin-like growth factor-1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Higashi Y, Pandey A, Goodwin B, Delafontaine P 
In:
Biochim Biophys Acta (2013) 1832(3): 391-9 
Estradiol inhibits vascular endothelial cells pro-inflammatory activation induced by C-reactive protein
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cossette É, Cloutier I, Tardif K, DonPierre G, Tanguay JF. 
In:
Mol Cell Biochem (2013) 373(1-2):: 137-47 
Differential effects of estrogen receptor ligands on regulation of dihydrotestosterone-induced cell proliferation in endothelial and prostate cancer cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Weng C, Cai J, Wen J, Yuan H, Yang K, Imperato-McGinley J, Zhu YS. 
In:
Int J Oncol. (2013) 42(1):: 327-37 
Differential effects of estrogen receptor ligands on regulation of dihydrotestosterone-induced cell proliferation in endothelial and prostate cancer cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Weng C, Cai J, Wen J, Yuan H, Yang K, Imperato-McGinley J, Zhu YS 
In:
Int J Oncol. (2013) 42(1): 327-37 
Production and Assessment of Decellularized Pig and Human Lung Scaffolds
Categories:
 
Authors:
Joan E. Nichols, PhD, Jean Niles, MA, Michael Riddle, MD, Gracie Vargas, PhD, Tuya Schilagard, PhD, Liang Ma, PhD, Kert Edward, PhD, Saverio La Francesca, Jason Sakamoto, PhD, Stephanie Vega, BS, Marie Ogadegbe, BS, Ronald Mlcak, PhD, Donald Deyo, DVM, Lee Woodson, MD, Christopher McQuitty, MD, Scott Lick, MD, Daniel Beckles, MD, Esther Melo, MA, and Joaquin Cortiella MD, MPH 
In:
Tissue Eng (2013) 19: 2045-2062 
Differential susceptibility of human primary aortic and coronary artery vascular cells to RNA interference.
Categories:
Primary Cells and Media 
Authors:
Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW 
In:
Biochim Biophys Acta (2012) 425(2): 261-5 
Integration of basal topographic cues and apical shear stress in vascular endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morgan JT, Wood JA, Shah NM, Hughbanks ML, Russell P, Barakat AI, Murphy CJ. 
In:
Biochem Biophys Res Commun (2012) 33(16): 4126-35 
Aronia melanocarpa fruit extract exhibits anti-inflammatory activity in human aortic endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zapolska-Downar D, Bryk D, Malecki M, Hajdukiewicz K, Sitkiewicz D. 
In:
Eur J Nutr (2012) 51(5): 563-72 
Nicotine-mediated induction of E-selectin in aortic endothelial cells requires Src kinase and E2F1 transcriptional activity
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alamanda V, Singh S, Lawrence NJ, Chellappan SP. 
In:
Biochem Biophys Res Commun (2012) 418(1): 56-61 
Vaspin increases nitric oxide bioavailability through the reduction of asymmetric dimethylarginine in vascular endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jung CH, Lee WJ, Hwang JY, Lee MJ, Seol SM, Kim YM, Lee YL, Kim HS, Kim MS, Park JY. 
In:
PLoS ONE (2012) 7(12): e52346 
MicroRNA changes in human arterial endothelial cells with senescence: relation to apoptosis, eNOS and inflammation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rippe C, Blimline M, Magerko KA, Lawson BR, LaRocca TJ, Donato AJ, Seals DR. 
In:
Exp Gerontol. (2012) 47(1): 45-51 
A key role for the endothelium in NOD1 mediated vascular inflammation: comparison to TLR4 responses
Categories:
Primary Cells and Media 
Authors:
Gatheral T, Reed DM, Moreno L, Gough PJ, Votta BJ, Sehon CA, Rickard DJ, Bertin J, Lim E, Nicholson AG, Mitchell JA. 
In:
PLoS ONE (2012) 7(8): e42386 
Pathogen-mediated proteolysis of the cell death regulator RIPK1 and the host defense modulator RIPK2 in human aortic endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Madrigal AG, Barth K, Papadopoulos G, Genco CA. 
In:
PLoS Pathog (2012) 8(6): e1002723 
TNF-a response of vascular endothelial and vascular smooth muscle cells involve differential utilization of ASK1 kinase and p73
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rastogi S, Rizwani W, Joshi B, Kunigal S, Chellappan SP 
In:
Cell Death Differ (2011) 19(2): 274-83 
Systemic activation of the transient receptor potential vanilloid subtype 4 channel causes endothelial failure and circulatory collapse: Part 2
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Willette RN, Bao W, Nerurkar S, Yue TL, Doe CP, Stankus G, Turner GH, Ju H, Thomas H, Fishman CE, Sulpizio A, Behm DJ, Hoffman S, Lin Z, Lozinskaya I, Casillas LN, Lin M, Trout RE, Votta BJ, Thorneloe K, Lashinger ES, Figueroa DJ, Marquis R, Xu X. 
In:
J Pharmacol Exp Ther (2011) 338(1): 408-9 
FGF-dependent regulation of VEGF receptor 2 expression in mice
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
MurakamiMurakami M, Nguyen LT, Hatanaka K, Schachterle W, Chen PY, Zhuang ZW, Black BL, Simons M M1, Nguyen LT, Hatanaka K, Schachterle W, Chen PY, Zhuang ZW, Black BL, Simons M 
In:
J Clin Invest (2011) 121(7): 2668-78 
Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mendonça MC, Koles N, Doi SQ, Sellitti DF. 
In:
Am J Physiol Heart Circ Physiol (2010) 299(6): H2018-27 
Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool
Categories:
Primary Cells and Media 
Authors:
Shahani T, Lavend\'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. 
In:
Blood (2010) 115(23): 4902-9 
Buthionine sulfoximine promotes methylglyoxal-induced apoptotic cell death and oxidative stress in endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Takahashi K1, Tatsunami R, Oba T, Tampo Y. 
In:
Biol Pharm Bull (2010) 33(4): 556-60 
MicroRNA 217 modulates endothelial cell senescence via silent information regulator 1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Menghini R, Casagrande V, Cardellini M, Martelli E, Terrinoni A, Amati F, Vasa-Nicotera M, Ippoliti A, Novelli G, Melino G, Lauro R, Federici M. 
In:
Circulation (2009) 120(15): 1524-32 
Release of proinflammatory mediators and expression of proinflammatory adhesion molecules by endothelial progenitor cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang Y, Ingram DA, Murphy MP, Saadatzadeh MR, Mead LE, Prater DN, Rehman J. 
In:
Am J Physiol Heart Circ Physiol (2009) 296(5): H1675-82 
Premature senescence of highly proliferative endothelial progenitor cells is induced by tumor necrosis factor-alpha via the p38 mitogen-activated protein kinase pathway.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang Y, Herbert BS, Rajashekhar G, Ingram DA, Yoder MC, Clauss M, Rehman J. 
In:
FASEB J (2009) 23(5): 1358-65 
Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hao Q, Wang L, Zhao ZJ, Tang H. 
In:
J Biol Chem (2009) 284(2): 799-806 
Oxygen Deprivation Triggers Upregulation of Early Growth Response-1 by the Receptor for Advanced Glycation End Products
Categories:
Transfection 
Authors:
Chang JS, Wendt T, Qu W, Kong L, Zou YS, Schmidt AM, Yan SF 
In:
Circ Res (2008) 102(8): 905-13 
Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells
Categories:
Transfection 
Authors:
Iversen N, Birkenes B, Torsdalen K and Djurovic S 
In:
Genet Vaccines Ther (2005) 3(1): 2 
Nod1-Mediated Endothelial Cell Activation by Chlamydophila Pneumoniae
Categories:
Transfection 
Authors:
Opitz B, Forster S, Hocke AC, Maass M, Schmeck B, Hippenstiel S, Suttorp N and Krull M 
In:
Circ Res (2005) 96(3): 319-326 
A novel in vitro system, the integrated discrete multiple organ cell culture (IdMOC) system, for the evaluation of human drug toxicity: comparative cytotoxicity of tamoxifen towards normal human cells from five major organs and MCF-7 adenocarcinoma breast cancer cells.
Categories:
Primary Cells and Media 
Authors:
Li AP1, Bode C, Sakai Y 
In:
Chem Biol (2004) 150(1): 129-36 
Endothelial cell diversity revealed by global expression profiling
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
PHACTR1 splicing isoforms and eQTLs in atherosclerosis-relevant human cells.
Categories:
Primary Cells and Media 
Authors:
Codina-Fauteux VA1,2, Beaudoin M1, Lalonde S1, Lo KS1, Lettre G3,4. 
In:
BMC Genet () 1: 97 
Flavorings in Tobacco Products Induce Endothelial Cell Dysfunction.
Categories:
Primary Cells and Media 
Authors:
Fetterman JL1, Weisbrod RM2, Feng B2, Bastin R2, Tuttle ST2, Holbrook M2, Baker G2, Robertson RM3, Conklin DJ4, Bhatnagar A3, Hamburg NM2 
In:
Arterioscler Thromb Vasc Biol () 7: 1607-1615 
CRP Stimulates GDF15 Expression in Endothelial Cells through p53.
Categories:
Primary Cells and Media 
Authors:
Kim Y1, Noren Hooten N1, Evans MK1. 
In:
Mediators Inflamm () 2018: 8278039 
Down-regulation of vascular GLP-1 receptor expression in human subjects with obesity.
Categories:
Primary Cells and Media 
Authors:
Kimura T1, Obata A2, Shimoda M2, Shimizu I3, da Silva Xavier G4, Okauchi S2, Hirukawa H2, Kohara K2, Mune T2, Moriuchi S2, Hiraoka A5, Tamura K5, Chikazawa G5, Ishida A5, Yoshitaka H5, Rutter GA4, Kaku K2,6, Kaneto H2 
In:
Scientific Reports () 1: 10644 
Ganglioside GM1 contributes to extracellular/intracellular regulation of insulin resistance, impairment of insulin signaling and down-stream eNOS activation, in human aortic endothelial cells after short- or long-term exposure to TNFa.
Categories:
Primary Cells and Media 
Authors:
Sasaki N1, Itakura Y1, Toyoda M1 
In:
OncoTarget () 5: 5562-5577 
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Categories:
Primary Cells and Media 
Authors:
Hitzel J1,2, Lee E3,4, Zhang Y3,5, Bibli SI2,6, Li X7, Zukunft S2,6, Pflüger B1,2, Hu J2,6, Schürmann C1,2, Vasconez AE1,2, Oo JA1,2, Kratzer A8,9, Kumar S10, Rezende F1,2, Josipovic I1,2, Thomas D11, Giral H8,9, Schreiber Y12, Geisslinger G11,12, Fork C1,2, Yang X13, Sigala F14, Romanoski CE15, Kroll J7, Jo H10, Landmesser U8,9,16, Lusis AJ17, Namgaladze D18, Fleming I2,6, Leisegang MS1,2, Zhu J19,20, Brandes RP21,22 
In:
Nat Commun. () 9: 2292 
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