Endothelial, aortic, human (HAEC)

Endothelial cells isolated from human aorta

Cell Type:
Aortic Endo.
Tissue Origin:
aortic
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial cell media are low serum media optimized for the proliferation of endothelial cells.

EGM-2 is a refined version of EGM.
 - Does not contain BBE
 - Final serum concentration is 2%
 - Improved cell proliferation over EGM

EGM-2 can be used to grow all of Clonetics Endothelial Cells except microvascular, coronary artery, umbilical artery, and iliac artery

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P5

EH-100

1e5 73% 70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P5 EH-100 1e5 73% ±8 70% ±8 Plasmid (general) 0.4 µg 20 µl Shuttle
AD1 EG-142 1e5 85% 85% Plasmid (general) 17 µg 350 µl 4D Y-Unit

Citations (50)

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Primary Cells and Media 
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Primary Cells and Media 
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