Natural killer Cells (NK), human

Specialized periphal blood lymphocytes that recognize and destroy foreign cells or infected host cells in a nonspecific manner.

Natural killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation. Since they are part of innate immunity, they do not require sensitization for the expression of their activity. NK cells play significant roles in viral infections, autoimmunity, pregnancy, cancer, bone marrow transplantation, and more recently adaptive immunity. NK cells are most traditionally characterized by the presence of surface marker CD56.

Cell Type:
Natural Killer (NK)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
NK cell, human U-001 2e6 16% ±4 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
P3 EK-100 5e5 30-32% 76% Plasmid (general) 400 ng 20 µl 4D X-Unit
P3 FA-100 5e5 53-54% 44-45% Plasmid (general) 400 ng 20 µl 4D X-Unit
P3 EN-138 3e6 35.5% 77.5% Plasmid (general) 20 µl 4D X-Unit

Citations (15)

Categories:
Transfection 
Authors:
Mélanie Lambert , Caroline Leijonhufvud , Filip Segerberg , J Joseph Melenhorst , Mattias Carlsten  
In:
Methods Mol Biol (2020) 2121: 213-219 
Categories:
Transfection 
Authors:
Prager I, Liesche C, van Ooijen H, Urlaub D, Verron Q, Sandström N, Fasbender F, Claus M, Eils R, Beaudouin J, Önfelt B, Watzl C. 
In:
J Exp Med (2019) 216(9): 2113-2127 
Categories:
Primary Cells and Media, Transfection 
Authors:
Naeimi Kararoudi, M., Dolatshad, H., Trikha, P., Hussain, S. R., Elmas, E., Foltz, J. A., Moseman, J. E., Thakkar, A., Nakkula, R. J., Lamb, M., Chakravarti, N., McLaughlin, K. J., Lee, D. A. 
In:
Journal of Visualized Experiments, JoVE (2018) 136: e58237 
Categories:
Transfection 
Authors:
Zhao Y1, Hu J2, Li R1, Song J1, Kang Y1, Liu S1, Zhang D1. 
In:
OncoTarget (2015) 8: 1553-9 
Categories:
Transfection 
Authors:
Chu Y, Hochberg J, Yahr A, Ayello J, van de Ven C, Barth M, Czuczman M, Cairo MS. 
In:
Cancer Immunol Immunother (2015) 3(4): 333-44 
Categories:
Primary Cells and Media 
Authors:
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Imai K, Hayashi T, Nakachi K, Young LF, Moore MA, van den Brink MR, Kusunoki Y. 
In:
J Immunol (2013) 190(12): 6164-72 
Categories:
Primary Cells and Media 
Authors:
Deuse T, Seifert M, Phillips N, Fire A, Tyan D, Kay M, Tsao PS, Hua X, Velden J, Eiermann T, Volk HD, Reichenspurner H, Robbins RC, Schrepfer S. 
In:
Circulation (2011) 124(11): ePub 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A. 
In:
Blood (2008) 111(3): 1428-36 
Categories:
Transfection 
Authors:
Marusina AI, Burgess SJ, Pathmanathan I, Borrego F, Coligan JE 
In:
J Immunol (2008) 180(1): 409-17 
Categories:
Transfection 
Authors:
Khurana D, Arneson LN, Schoon RA, Dick CJ, Leibson PJ 
In:
J Immunol (2007) 178(6): 3575-82 
Categories:
Transfection 
Authors:
Radons J, Gross C, Stangl S and Multhoff G 
In:
J Immunol Methods (2005) 303(1-2): 135-141 
Categories:
Transfection 
Authors:
Trompeter HI, Gomez-Lozano N, Santourlidis S, Eisermann B, Wernet P, Vilches C and Uhrberg M 
In:
J Immunol (2005) 174(7): 4135-4143 
Categories:
Transfection 
Authors:
Mirandola P, Ponti C, Gobbi G, Sponzilli I, Vaccarezza M, Cocco L, Zauli G, Secchiero P, Manzoli FA and Vitale M 
In:
Blood (2004) 104(8): 2418-2424 
Categories:
Transfection 
Authors:
Roda-Navarro P, Mittelbrunn M, Ortega M, Howie D, Terhorst C, Sanchez-Madrid F and Fernandez-Ruiz E 
In:
J Immunol (2004) 173(6): 3640-3646 
Categories:
Transfection 
Authors:
Trompeter HI, Weinhold S, Thiel C, Wernet P and Uhrberg M 
In:
J Immunol Methods (2003) 274: 245-256 
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