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Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

No. SDS has a negative influence on Adenylate Kinase (AK) activity. SDS is a cationic detergent, it is a very powerful protein denaturing agent, you will be aware of its use in SDS-PAGE for those reasons. ToxiLight™ works by measuring the activity of...

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

The DNA bands on my agarose gel are sometimes wavy, but usually only in one or two lanes. What causes this?

Dried agarose on the comb teeth is a frequent cause of this problem. Prior to casting your gel, check the comb teeth for residual dried agarose.If not removed, this will attach to the newly cast agarose and fracture the well upon comb removal. This...

What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?

The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...

What are mycoplasmas?

Mycoplasmas belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "Mycoplasma" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct Mycoplasma...

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...

How often do mycoplasma contaminations occur in cell cultures?

A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

How many samples can I transfect in parallel when using the 4D-Nucleofector® X Unit?

The 4D-Nucleofector® X Unit offers flexible throughput from 1 to 16 samples. When working with 100 µl single Nucleocuvettes® you can process two vessels in parallel. However subsequent handling of more than 2 vessels is eneased via the option to...

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