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Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLight™ Assay?

No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.

When preparing to run a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit how is the potency of CSE determined?

The RSE/CSE procedure used by Lonza to determine CSE potency is found on the Certificate of Analysis for each matched kit. In general, dilutions of endotoxin from multiple vials of CSE are tested unknowns compared to a series of standards produced...

What temperature should the ToxiLight™, ViaLight™ and MycoAlert™ Assays be run at?

The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I handle the LAL reagent?

Lonza LAL reagent should be handled gently. After reconstitution, the contents of the vial will easily go into solution by either gently inverting or swirling the vial. Vortexing or vigorously shaking the reconstituted LAL reagent will cause it to...

What types of gels do you have for the FlashGel™System?

We offer FlashGel™ Cassettes for DNA, DNA recovery and RNA separation. FlashGel™ DNA Cassettes are available at either 1.2% or 2.2% agarose, and in 12+1 single-tier (13 wells) or 16+1 double-tier formats (34 wells). FlashGel™ DNA Recovery Cassettes...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I prepare my sample for testing?

There are a few ways in which your sample can be prepared before testing it. Most samples only need to be diluted before they are tested with one of our endotoxin detection kits. In order to determine how far out you should dilute your sample, you...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay what pH should my sample be? What should I adjust it with?

Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by...

What is the difference between the EBM™ and EBM™-2 Basal Media? Can the EBM™-2 Basal Media be substituted with the EBM™ phenol red free Basal Media?

The EBM™ and EBM™-2 Basal Media are essentially identical. The major difference between the EBM™ and EBM™-2 Basal Media is the concentration of phenol red (the EBM™-2 Basal Media has about 1/10th the amount of phenol red as the EBM™ Basal Media). The...

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...

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