Q:

Is it possible to convert the B/A value of positive control to CFU of Mycoplasma?

A:

The assay is not quantitative so you cannot relate the ratio to an actual value , different species will give

different ratios due to levels of expression of the enzymes we measure - the assay just gives a Yes or No

answer. The positive control is designed to just give a positive ratio.

Categories:
Bioassay