faq

Q:	Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?
A:	The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins. For optimal assay performance, supernatant should be tested as soon as possible after collection. Ideally you should sample your cell cultures and test immediately. If needed you can store the cell free supernatant in the following way: cell free supernatant can be kept at room temperature or 4°C for testing same day cell free supernatant can be frozen and stored at -80°C for 6 months. For this purpose, freeze supernatant immediately after spinning out cells. Thaw the sample without the aid of artificial heat and equilibrate to room temperature for at least 15 minutes before testing.

Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.

For optimal assay performance, supernatant should be tested as soon as possible after collection. Ideally you should sample your cell cultures and test immediately. If needed you can store the cell free supernatant in the following way:

cell free supernatant can be kept at room temperature or 4°C for testing same day
cell free supernatant can be frozen and stored at -80°C for 6 months. For this purpose, freeze supernatant immediately after spinning out cells. Thaw the sample without the aid of artificial heat and equilibrate to room temperature for at least 15 minutes before testing.

Categories:

Bioassay