Fresh supernatant and testing the same day is still the recommended procedure as mycoalert was
designed as a fast preliminary screen for mycoplasma. For this the supernatant can be kept at room
temperature or 4oC until the end of the day before testing.
If sample storage is required:
< 1 week recommend 4oC.
> 1 week recommend freezing at -20oC.
Supernatant Only! - cells MUST be removed at 1,500rpm (200xg) for 5 mins prior to storage.