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What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using the...

When would be the optimal time to perform experiments post osteogenic differentiation for human mesenchymal stem cells?

Once the cells have differentiated and started laying down bone matrix, we recommend assaying the cells immediately. If cells begin to delaminate (peel of the culture plate in layers), this would indicate that the cells are embedded in the calcium...

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...

Why do we recommend that MycoZap™ Reagent 1 treatment must be conducted in media containing a maximum of 5% (v/v) serum? What happens if by mistake a higher serum percentage was used?

As serum is complexing the MycoZap™ Reagent 1 you would decrease the effective concentration and thus inhibit the elimination reaction. If by mistake the elimination was started with higher serum concentration, no elimination took place. The process...

Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?

Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...

Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...

How much data can be stored on the Lucetta® 2 Luminometer AAL-1002

The Lucetta 2 Luminometer has a high data storage capacity with maximum storage of up to 2000 single sample readings.

How old are the donors for Lonza’s dermal cells from neonatal donors?

The neonatal foreskin tissue used to isolate Lonza’s dermal cells from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed before...

Are the human mesenchymal stem cells (PT-2501) more prone to become differentiated down one lineage over the other?

MSCs are prone to differentiate down an adipogenic lineage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...

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