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Yes. Please see our Techincal Reference Guide which contains many helpful tips.You can find additional information in the Optimized Nucleofection Protocol for your cell type of interest.
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The ViaLight™ Plus Cell Lysis Reagent inhibits ATPase activity - mainly by using a low pH and denaturing the cellular proteins. This is why the ViaLight™ Plus Assay has such a good half life. So cell expressing high ATPase will probably not...
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ToxiLight does not need a standard curve. You can just plot the RLUs produced including an untreated control. If you want you can create a 100% lysis control by using the ToxiLight™ 100% Lysis Control Set LT07-517 or by heating a...
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The assay has been developed in the presence of phenol red so all the example data has been conducted in complete media. Phenol red does quench the light signal so if you compared assays in phenol red free media they would have higher RLUs. The...
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Yes, all of our reagents include in ToxiLight™ Non-Destructive Cytotoxicity BioAssay, the ViaLight™ Plus BioAssay, the MycoAlert™ and MycoAlert™ Plus Assay, the PPiLight™ Inorganic Pyrophosphate Assay and the PDELight™ HTS cAMP Phosphodiesterase...
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It is possible that the PCR is picking up dead mycoplasma whereas the MycoAlert® and MycoAlert® Plus Assay will pick up only viable mycoplasma. It is possible of course that there is contamination in the PCR giving a false positive.
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For supernatants there will be exogenous ATPases present that will degrade ATP so it should really be treated as if it were cells. Freeze as quickly as possible and store at -80C, for no more than 4 weeks. The ATP will be OK with the freezing.
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The MycoAlert® and MycoAlert® PLUS Assay are not quantitative so you cannot relate the ratio to an actual value. Different mycoplasma species will give different ratios due to different levels of expression of the enzymes we measure -...
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The main inhibitors of the MycoAlert® Reaction would be things that affect the luciferase detection. Agents which potentially quench the light signal include high levels of salt, EDTA and other chelating agents, very high levels of sera,...
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The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
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