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514 results sorted by
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Lonza’s Renal Proximal Tubule Epithelial Cells (RPTEC) are isolated from the cortex of the kidney. A characteristic of kidney cortex epithelial isolation results in a population consisting of approximately 50%- 70% RPTEC. Lonza uses a proprietary...
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Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...
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The human prostate can generally be described as being glandular epithelium (parenchymal) surrounded by a fibromuscular stroma.The parenchymal portion of the prostate, or of any organ in the body, is epithelial like in nature and contains the “worker...
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Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...
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Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...
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While Lonza has never attempted to co-culture Prostate Epithelial Cells (PrEC) and Prostate Stromal Cells (PrSC) together in-house, the recommendation of Lonza Research & Development would be to plate and culture the PrEC and PrSC cells together...
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A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...
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MSCs are prone to differentiate down an adipogenic lineage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...
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Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
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Lonza’s Prostate Stromal Cells (PrSC) are isolated from the stroma of the prostate. Lonza’s Prostate Stromal Cells (PrSC) are a mixed population of cells, however, the majority of the cells in this population are fibroblastic in origin. It is not...
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