Search Results

Which Nucleofector® Protocol should I use for genome editing and transfection of CRISPR/Cas9 into my cells?

Please use always the ready-to-use protocol for the specific cell type. The optimized programs are cell type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins with...

Where are Lonza’s Ventricular Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Ventricular Normal Human Cardiac Fibroblast (NHCF-V) from left ventricle.

Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...

Where are Lonza’s Atrial Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Atrial Normal Human Cardiac Fibroblast (NHCF-A) from right atrium.

Does the basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) contain proteins?

The basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (MSCGM™), catalog no BEBP12-934Q, contains recombinant and human serum derived proteins.

Do you have a Nucleofection® Protocol for bacterial transformation for the Nucleofection® I, IIN, IIS or 2b Systems?

Yes, we do have guidelines for bacteria transformation on the Nucleofector® Systems. However, not all models of the Nucleofector® System series are capable of bacteria transformation and have bacteria pulses implemented in the program list,...

Will Lonza’s HSMM cells de-differentiate after they have been differentiated to form myotubes?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Additionally, differentiation is not...

What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem cells?

Lonza currently validates trilineage differentiation of human mesenchymal stem cells using the following quantitative assays:Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009)Osteogenic Differentiation: inorganic...

Do you have a Nucleofection® Protocol for transfection of yeast (Saccharomyces cerevisiae)?

Unfortunately we do not have any records about Nucleofection® of yeast. It is also impossible to search for current publications, because you get only false positive hits. A lot of yeast genes have been nucleofected and many reportergene systems have...

Where are Lonza's human Mesenchymal Stem Cells isolated from?

Lonza's human Mesenchymal Stem Cells (hMSCs) PT-2501 are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers between the ages of 18 and 45 years old. Please see the linked document for more...

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