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Preparation of coverslips: At Lonza we used coverslips from Menzel (part of Thermo Scientific). We think that the coverslips have such a good quality that they don’t have to be cleaned prior to use.If you want to use other coverslips or prefer...
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Lonza’s rat cardiac myocytes can also be cultured on fibronectin or laminin. Nitrocellulose was selected as the recommended substrate for its convenience and ease of use.
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There is no specification on whether the tissue for Lonza’s PASMCs comes from the area closest to the heart or the area closest to the lung.Generally, the tissue is pulled from the area branching off the heart so long as Lonza receives a long enough...
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Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.
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Lonza has observed these cells up to 59 days in culture and even at that time, the cells displayed beating in syncytium. It is, however, possible that unseen physiological changes may occur to the cells during this time. As these cells are a...
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Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...
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No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
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Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
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Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
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The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.
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