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How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.

Do I need to purchase a special chamber to use Reliant™ and Latitude™ Precast Gels?

Reliant™ and Latitude™ Precast Agarose Gels are designed to run in standard horizontal electrophoresis chambers.As long as there is room on the chamber platform for the gel, the chamber should be suitable. Measure the chamber platform and check...

What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.

Are there any substitutions that can be used in place of the HSA in the freezing media for hMSC's (Human Mesenchymal Stem Cells)?

Cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend...

Are the materials in the FlashGel™ Cassette hazardous?

The stain in the FlashGel™ Cassette is present at such low levels that it is not considered hazardous according to OSHA and EU hazard criteria. A copy of the MSDS is available online. However, as the stain in the cassette is a potential mutagen, wear...

Should any type of extracellular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem Cells)?

That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...

I stained my gel with SYBR Green and recovered my band of interest. However, my DNA is still green. Can I remove the SYBR stain?

Yes. The SYBR stains can easily be removed from nucleic acids by ethanol precipitation. Isopropyl alcohol precipitation is somewhat less effective at removing the dye. Butyl alcohol extraction, chloroform extraction and phenol extraction do not...

What is the difference between the different cell sources (bone marrow, cord blood, or fetal liver) for CD34+ cells?

Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive cells useful in transplant and stem cell research.Fetal Liver: best for developmental studies and they are highly proliferative....

What are the benefits of using agarose for protein gel electrophoresis?

Protein electrophoresis in agarose gels is an alternative approach to using polyacrylamide gels and provides several benefits: - Separate high molecular weight proteins (>600 kDa)- Easy to prepare and handle- Efficient recovery of proteins-...

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