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I am using your ProSieve™ Color Protein Markers and the molecular weights (MW) of my protein of interest is not what I expected when compared to the ProSieve™ Markers. Why is this?

The proteins in our ProSieve™ Color Markers are made of proteins that are individually prestained using colored dyes.The level of dye bound can affect the migration of the protein and this can vary slightly lot to lot. Therefore, it is important to...

What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.

Are there any substitutions that can be used in place of the HSA in the freezing media for hMSC's (Human Mesenchymal Stem Cells)?

Cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend...

Should any type of extracellular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem Cells)?

That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...

What types of gels do you have for the FlashGel™System?

We offer FlashGel™ Cassettes for DNA, DNA recovery and RNA separation. FlashGel™ DNA Cassettes are available at either 1.2% or 2.2% agarose, and in 12+1 single-tier (13 wells) or 16+1 double-tier formats (34 wells). FlashGel™ DNA Recovery Cassettes...

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...

What is the difference between the different cell sources (bone marrow, cord blood, or fetal liver) for CD34+ cells?

Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive cells useful in transplant and stem cell research.Fetal Liver: best for developmental studies and they are highly proliferative....

What are Lonza's human Mesenchymal Stem Cells (PT-2501) cryopreserved in?

The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.
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