That will depend on the type of medium used.
If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel.
If you are culturing the cells in our TheraPEAKTM MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (BEBP18-936), for peak performance, a 5ug/mL fibronectin coating could be used.