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Are there any contaminating glial cells in your Clonetics rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...

How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...

Do your Clonetics Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.

Do you have data about the in vivo implantation of hepatocytes transfected by Nucleofection? For how long could protein expression be detected?

There is a publication reporting on the successful reinjection of modified hepatocytes into mice.Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the...

Do you have any instructions available for using a hemocytometer for cell counting?

Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.

Are rat and mouse hepatocytes transfected by Nucleofection® still functional?

Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected cells. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection®.

What age are the mice and rats that your Clonetics Mouse and Rat Astrocytes are isolated from?

They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.


When I transfected my cells, error 8 appeared on the display of the Nucleofector® IIN, IIS or 2b. What is the reason?

Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...

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