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When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay?

Each endotoxin detection assay requires the use of different equipment. If running one of our PYROGENT™ Gel-Clot assays, a non-circulating waterbath or a dry heat block able to heat at 37°C is required. If running one of our kinetic assays,...

Can I get a Material Data Safety Sheet (MSDS) for my Lonza endotoxin detection Bacterial Endotoxin Testing (BET) kit, LAL or PyroGene™ Recombinant Factor C (rFC), and/or my accessory products?

All of our endotoxin detection products have been evaluated and deemed non-hazardous in accordance with 29 CFR 1910.1200, the Hazard Communication Standard, based on the percentage quantities of its constituents. Therefore, none of our endotoxin...

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay why should I use glass accesory products instead of plastic?

Endotoxin adheres to plastic surfaces more strongly than to glass surfaces. Therefore, we recommend that you use only borosilicate glass dilution tubes when preparing your Control Standard Endotoxin (CSE) dilutions. In some cases, certain plastics...

Where can I view a list of upcoming Lonza webinars? How can I access archived Lonza webinars?

To see our upcoming webinars go to Free Webinars | Lonza . This page also contains a link for viewing archived webinars. To receive upfront information on upcoming Webinars of Lonza Bioscience, just subscribe at Lonza...

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed in...

Is it possible to automate the PyroTec Pro standard dilutions?

The standard endotoxin dilutions are created by the system based on what is specified in the WinKQCL™ Software Template specific for that assay. The PyroTec™ PRO System can dynamically create the dilution scheme in the WinKQCL™ Software.The system...

Can you provide more information on the PyroTec Pro validation of endotoxin standards made using pipetting vs. vortexing?

The only manual step required is for the analyst to perform the initial reconstitution and vortex the initial stock CSE (or RSE) solution prior to running the assay. The robot prepares the standard curve. As we do not place a vortex mixer on the...

How does PyroTec Pro liquid level detection work if different sample containers are used (conductive or pressure based)?

Conductive liquid detection is used by the PyroTec PRO™ System. The system currently supports sample handling in 13 x 100 mm tubes. An option to allow use of additional 5 ml tubes with caps is planned for a future release.

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