Q:

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit should I use?

A:
There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only, then you may want to consider using our Endotoxin Testing Service. If you plan to test on a regular basis, then you first need to evaluate the equipment that you currently have. (See below: What equipment do I need to run my assay?) If you have multiple types of equipment available for your use, consider the type of results you wish to obtain. If you must have a quantitative result, then realize that gel clot kits will not suit your needs. Gel clot tests are capable of giving only a less than or more than result. By running multiple dilutions of a sample, you may be able to narrow the EU/ml result down to a range, but gel clot kits are, at best, semi-quantitative. Assay sensitivity is another important consideration. Our kinetic chromogenic LAL assay is our most sensitive: Kinetic-QCL™ can read down to 0.005 EU/ml. PYROGENT™-5000 and PyroGene™ assays can both read to 0.01 EU/ml, and QCL-1000™ can read to 0.1 EU/ml. PYROGENT™ gel clot assays can be ordered in various sensitivities: 0.03, 0.06, 0.125, and 0.25 EU/ml. Many customers have the option to use either QCL-1000™ or PYROGENT™. If this is the case, decide if sensitivity or a quantitative result is more important to you. If you will be testing many samples on a frequent basis, then you may want to consider purchasing equipment and software that you do not already have. Our kinetic assays and PyroGene™ rFC require less hands-on time than PYROGENT™ Gel Clot or QCL-1000™, with the benefit of increased sensitivity. Lonza sells both kinetic and fluorescent microplate readers, as well as our WinKQCL™ software specifically written for endotoxin-detection tests.
Related URLs:
Categories:
Endotoxin Detection
Research Areas:
Endotoxin Testing