Q:

What temperature should the MycoAlert® Assay be run at?
A:

Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use a water bath set to optimal temperature.
Categories:
Mycoplasma Detection and Prevention

You may also want to know:

Is it possible to quantify the mycoplasma with the MycoAlert® Mycoplasma Detection Assay?
What are mycoplasmas?
Do the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits detect dead mycoplasma?
Can mycoplasma grow outside of a cell?
Can mycoplasma grow in the absence of cells?
How can cells be cured of mycoplasma contamination?
How can cells be cured of mycoplasma contamination?
I tested my cell culture supernatant negative for mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit or the MycoAlert® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?
How can I detect mycoplasma contamination of my cells?
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